大鼠放射性脑损伤磁共振成像的初步研究

发布时间：2018-05-11 19:44

本文选题：磁共振成像 + 伽马刀　；参考：《安徽医科大学》2013年硕士论文

【摘要】：目的：研究伽玛刀照射大鼠尾状核后放射性损伤的影像学表现、一般情况改变及细胞学变化。方法：10只成年SD大鼠，5只大鼠作为对照组，5只大鼠接受MASEP-SRRS型伽玛刀8mm准直器以50Gy（50%等剂量线）照射大鼠右侧尾状核。在大鼠照射后第4周及第12周时分别接受1.5T MRI轴位平扫和增强扫描，，后取脑组织行HE染色观察细胞学变化。结果： 1.照射组大鼠食欲减低，活动稍减少，毛色差及不同程度的脱毛。照射组接受伽玛刀治疗前体重370.6±14.5g，接受伽玛刀治疗12周后体重313.8±17.5g，t=0.2767，P0.01，差异有统计学意义，体重明显下降。对照组大鼠体重放疗前为372.5±12.5g，放疗后为396.9±15.6g。与照射组接受伽玛刀相比，体重差异显著（P0.01）。 2.大鼠在第4周时头颅MRI检查提示受照射区域呈轻度增高信号灶，周围组织轻度水肿。第12周行头颅MRI检查，T2WI可见受照靶区呈片状稍高信号，边界不清，临近侧脑室受压，增强TIWI受照靶区呈明显强化，强化不均匀。对照组大鼠MRI图像均未见明显异常。 3．大鼠在放疗后脑组织切片及HE染色光镜下显示大脑皮层神经细胞固缩，核仁不明显，伴有胶质细胞增生，水肿及少量淋巴细胞浸润。结论： MRI能够很好且较早的显示放射性脑损伤后的改变，对于伽马刀所致的放射性脑损伤诊断有重要临床意义。
[Abstract]:Aim: to study the radiologic features, general changes and cytological changes of radiation injury in caudate nucleus of rats after gamma knife irradiation. Methods five adult Sprague-Dawley rats were treated with MASEP-SRRS type gamma knife 8mm collimator (50 Gy / 50% isodose line) to radiate the right caudate nucleus. At the 4th week and 12th week after irradiation, the rats were scanned with 1.5T MRI axial plain scan and enhanced scan respectively. The brain tissues were taken for HE staining to observe the cytological changes. Results: 1. In the irradiation group, the appetite decreased, the activity slightly decreased, the hair color aberration and different degrees of depilation. The weight of radiation group was 370.6 卤14.5 g before gamma knife therapy and 313.8 卤17.5 g / 12 weeks after gamma knife treatment. The difference was statistically significant and the weight decreased significantly. The body weight of control group was 372.5 卤12.5 g before radiotherapy and 396.9 卤15.6 g after radiotherapy. There was a significant difference in body weight between radiation group and gamma knife group (P 0.01). 2. At the 4th week, MRI examination showed that the irradiated area was slightly increased in signal intensity, and the surrounding tissue was slightly edema. At the 12th week, MRI showed that the target area was slightly high signal intensity, the boundary was not clear, the adjacent ventricle was compressed, and the enhancement of TIWI was obvious and uneven on T2WI. There was no obvious abnormality in MRI images in the control group. 3. Brain tissue sections of rats after radiotherapy and light microscopy with HE staining showed that the neurons in the cerebral cortex were pyknosis, nucleolus was not obvious, glial cell proliferation, edema and a small amount of lymphocyte infiltration. Conclusion: MRI can show the changes after radiation brain injury very well and earlier, and has important clinical significance for the diagnosis of radiation brain injury caused by gamma knife.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R445.2;R818

【共引文献】