放射性脑损伤的MRS、PWI动物实验研究

发布时间：2018-05-12 14:15

本文选题：动物模型 + 放射性脑损伤　；参考：《青岛大学》2013年硕士论文

【摘要】：目的：建立放射性脑损伤的大鼠模型,研究MRS、PWI在放射性脑损伤不同时期的改变,为放射性脑损伤的早期诊断提供依据,并初步探讨放射性脑病可能的发病机制。材料与方法：将成年雌性Wistar大鼠30只随机分为空白对照组和单纯照射组,均进行MRI、MRS及MR-PWI检查。单纯照射组采用6Mev的电子线对大鼠进行20Gy单次全脑垂直照射,分别于照射后1、3、6、9及12个月行MRI、MRS及MR-PWI检查,分析N-乙酰天门冬氨酸(NAA)、胆碱(Cho)、肌酸(Cr)等含量的变化,采用积分面积进行比较,计算NAA/Cr和Cho/Cr (?)匕值,同时在MR后处理工作站获得PWI伪彩图,测量灌注后大鼠双侧侧脑室周围的rCBV值。结果：①所有被检查大鼠的大脑常规MRI均未见异常表现。②各照射组大鼠的NAA/Cr比值与对照组相比随时间延长逐渐下降,且均有统计学意义(P0.05)；照射后Cho/Cr比值先上升,3个月时达峰值,随后逐渐下降,6个月内各组的Cho/Cr与对照组相比有统计学意义(P0.05)。③照射后1个月组大鼠的rCBV与对照组相比无明显统计学意义。照射1个月后各组的rCBV值均明显低于对照组,差异有统计学意义(P0.05) 结论：①MRS能够在放射性脑损伤发生形态学改变之前,检测出脑组织代谢的异常 ②PWI能够在放射性脑损伤发生形态学改变之前,检测出脑组织微循环的异常。
[Abstract]:Objective: to establish a rat model of radiation brain injury and to study the changes of MRS PWI in different stages of radiation brain injury, to provide evidence for the early diagnosis of radiation brain injury, and to explore the possible pathogenesis of radiation encephalopathy. Materials and methods: thirty adult female Wistar rats were randomly divided into blank control group and irradiation group. MRI Mrs and MR-PWI were performed. Rats in the single irradiation group were exposed to 6Mev single whole brain vertical irradiation. MRIMRS and MR-PWI were performed at 1 and 12 months after irradiation to analyze the changes of the contents of N-acetylaspartate, choline chorion and creatine. The NAA/Cr and Cho/Cr are calculated by comparing the integral area. At the same time, the pseudochromatic PWI images were obtained on Mr postprocessing workstation, and the rCBV values around the bilateral ventricles of rats were measured after perfusion. Results no abnormal MRI was found in the brain of all the examined rats. 2 the NAA/Cr ratio of the rats in each irradiation group decreased with time, compared with that of the control group. The ratio of Cho/Cr increased firstly and reached the peak at 3 months after irradiation. The Cho/Cr of each group was significantly lower than that of control group within 6 months. The rCBV of rats in the group 1 month after P0.05.3 irradiation had no statistical significance compared with that of the control group. The rCBV value of each group was significantly lower than that of the control group one month after irradiation, and the difference was statistically significant (P 0.05). Conclusion 1 Mrs can detect the abnormal metabolism of brain tissue before the morphological changes of radiation brain injury. 2PWI can detect the abnormal microcirculation of brain tissue before the morphological changes of radiation brain injury.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R445.2;R818.74

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