大鼠运动性肌损伤特征microRNA表达及其膜损伤调控靶点的预测

发布时间：2018-05-12 20:42

本文选题：运动 + 骨骼肌　；参考：《生理学报》2017年03期

【摘要】：本文旨在筛选大鼠运动性肌损伤(exercise-induced muscle damage,EIMD)的特征microRNA(miRNA),并预测分析特征miRNA调控膜损伤的靶点。将24只健康雄性Sprague-Dawley(SD)大鼠平均分为安静对照(C)组、运动后24 h(E24)组、运动后48 h(E48)组,采取一次间歇性离心运动(-16°下坡跑台跑)复制EIMD模型,用伊文氏蓝荧光染色法鉴定EIMD特征,用基因芯片进行差异性miRNA筛选,用RT-q PCR验证芯片检测结果后获得EIMD差异性miRNA表达谱,RT-qPCR检测EIMD膜损伤相关特征蛋白和相关通路核心分子的m RNA表达水平并预测特征miRNA的靶基因。本研究筛选出并验证了2条EIMD特征miRNA:miR-206-3p和miR-139-3p。骨架蛋白dystrophin(r=-0.68)、utrophin(r=-0.64)和MAPK信号通路核心分子JNK(r=-0.62)、ERK1(r=-0.68)均与miR206-3p呈中度负相关(P0.001),而均与miR-139-3p无显著相关。以上研究结果提示,EIMD可诱导成年大鼠腓肠肌miRNA表达谱明显改变,miR-206-3p和miR-139-3p是EIMD特征miRNA,miR-206-3p可能通过调控骨架蛋白dystrophin、utrophin和MAPK信号通路分子JNK、ERK1调控膜损伤。
[Abstract]:The aim of this study was to screen the characteristic microRNAs of exercise-induced muscle damage (EIMD) in rats and to predict the targets of miRNA regulatory membrane damage. Twenty-four healthy male Sprague-Dawley (SD) rats were divided into two groups on average: control group (C), control group (24 h after exercise), and group E48 (48 h after exercise). The EIMD model was established by an intermittent centrifugal exercise (-16 掳downhill treadmill run). The characteristics of EIMD were identified by Yi Wen blue fluorescence staining. Gene chip was used to screen differential miRNA, and RT-q PCR was used to verify the results of EIMD differential miRNA expression profile. RT-PCR was used to detect the expression level of EIMD membrane damage related characteristic protein and the core molecule of related pathway, and to predict the target gene of characteristic miRNA. In this study, two EIMD features, miRNA:miR-206-3p and miR-139-3p. Both the skeleton protein dystrophin r-0.68 and the core molecule of MAPK signaling pathway, MAPK, were negatively correlated with miR206-3p (P 0.001), but had no significant correlation with miR-139-3p. These results suggest that EIMD can induce significant changes in the expression profile of miRNA in gastrocnemius muscle of adult rats. The changes of miR-206-3p and miR-139-3p are characteristic of EIMD. MiR-206-3p may regulate membrane damage by regulating the skeleton protein dystrophinutrophin and MAPK signaling pathway molecule JNK-ERK1.
【作者单位】：杭州师范大学体育与健康学院;北京体育大学运动人体科学学院;
【基金】：supported by the National Natural Science Foundation of China(No.31271276)
【分类号】：R873

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