不同运动负荷大鼠骨骼肌损伤及修复过程中血液肌酸激酶和乳酸脱氢酶变化的研究

发布时间：2018-05-13 04:11

本文选题：运动性骨骼肌损伤 + 损伤后修复　；参考：《吉林大学》2012年硕士论文

【摘要】：研究目的：探索大鼠经不同负荷（无负荷、中等强度、大强度）运动训练后，诱导其骨骼肌损伤，在自然修复期和药物（云南白药）治疗期血液中肌酸激酶（CK）和乳酸脱氢酶（LDH）的变化，为骨骼肌损伤的预防、损伤后的准确诊断及其治疗提供理论依据，为科学的体育锻炼提供理论基础。研究方法：对经过为期8周的训练的中等强度(Z)组、大强度(D)组和无负荷组的大鼠，建立大鼠运动损伤模型（参照田野运动训练损伤模型的建立方法）诱导其骨骼肌损伤。将每组大鼠随机为治疗组及非治疗组，治疗组从24小时至第10天给予喷洒云南白药气雾剂，非治疗组给予喷洒等剂量的生理盐水。上述三组大鼠治疗组和非治疗组损伤后按即刻、1天、4天、7天、10天，5个时间段取血测定血清肌酸激酶（CK）及乳酸脱氢酶（LDH）活性的变化。研究结果： 1、大负荷非治疗训练组大鼠的在损伤后即刻和修复期的1、4、7、10天CK值和LDH值较安静对照组高（P0.05）。与中等负荷非治疗组相比，大负荷非治疗组CK值和LDH值在损伤后即刻、损伤后第1天和第4天均较高（P0.05）；而在损伤后的第7天至10天大负荷组CK值和LDH值较低，但二者无显著性差异（P0.05）；与无负荷非治疗组相比，大负荷非治疗训练组的大鼠CK、LDH值在损伤后即刻较高，但他们之间无显著性差异（P0.05）；CK值和LDH值在损伤修复期的第4天较高（P0.05）。 2、大负荷治疗组大鼠在骨骼肌损伤后的第4天至10天CK值低于大负荷非治疗组(p0.05)；而LDH值则在第4天低于大负荷非治疗组(p0.05)。与中等负荷治疗组和无负荷治疗组相比，大负荷治疗组CK值和LDH值在损伤后4天时较高(p0.05)。 3、中等负荷非治疗组在骨骼肌损伤后即刻、损伤后的第1天、第4天血清CK值和LDH值高于安静对照组（P0.05）。损伤后的第7天至10天血清CK值和LDH值已接近安静对照组CK值，二者之间无显著性差异（P0.05）。 4、无负荷非治疗组骨骼肌损伤后即刻和损伤后的恢复期的第1天、4天、7天血清CK值高于安静对照组（P0.05），在损伤后的第10天CK酶值接近于安静对照组；而血清LDH则在骨骼肌损伤后即刻和损伤后的第1天、4天高于安静对照组（P0.05）。与中等负荷非治疗训练组相比，无负荷组大鼠非治疗组CK值和LDH值在损伤后即刻较高(p0.05)；损伤后的第1天至第10天该值虽有升高但无显著性差异（P0.05）。 5、无负荷治疗组在骨骼肌损伤后的第4天至10天CK值和LDH值均低于大负荷组大鼠非治疗组(p0.05)；高于损伤后的第4天的中等强度治疗训练组，但无显著性差异（P0.05）；7天至10天无负荷组CK值和LDH值接近于安静对照组。结论： 1．根据中等负荷非治疗组大鼠CK和LDH值较大负荷和无负荷训练非治疗组大鼠在损伤后即刻和修复期的第1天、4天低（P0.05），第7天和第10天无显著性差异的特征，表明中等负荷组大鼠损伤程度比大负荷和无负荷训练组轻，并且自然修复快。说明中等负荷组大鼠骨骼肌的抗损伤能力高于大负荷组和无负荷组。 2．根据大负荷训练的大鼠CK、LDH值在损伤后即刻较无负荷训练的大鼠高，但他们之间无显著性差异；在损伤后的4天至10天较无负荷训练的大鼠CK、LDH值要高（P0.01）的特征，我们可以认为大负荷训练组与无负荷组在损伤即刻其损伤程度没有统计学差异，但大负荷训练组在损伤4天以后自然修复速度较无负荷训练组要慢。表明大负荷组在8周的大负荷训练和诱导骨骼肌损伤过程中可能有重复性损伤，说明骨骼肌损伤具有积累效应。 3．根据大负荷组、中等负荷组和无负荷运动组大鼠骨骼肌损伤后经过云南白药治疗后的第4天、第7天、第10天CK酶和LDH酶均低于非治疗组(P0.05)的特征。说明云南白药能加速大鼠运动性骨骼肌损伤的修复。
[Abstract]:Objective: To explore the injury of skeletal muscle in rats after exercise training with different loads (no load, medium intensity and intensity). The changes of creatine kinase (CK) and lactate dehydrogenase (LDH) in the natural repair period and drug (Yunnan Baiyao) treatment period are the prevention of skeletal muscle injury, the accurate diagnosis and treatment of the injury after injury. On the basis of this, it provides a theoretical basis for scientific physical training.
Methods: the rats in the medium intensity (Z) group, the high intensity (D) group and the non load group were trained to induce the injury of the skeletal muscle in the rats (reference field training damage model). The rats in each group were randomly assigned to the treatment group and the non treatment group. The treatment group was given from 24 to tenth days. The non treatment group was sprayed with the spray of Yunnan Baiyao aerosol, and the non treatment group was sprinkled with equal dose of saline. The changes of serum creatine kinase (CK) and lactate dehydrogenase (LDH) activity were measured in the three groups of rats and non treatment groups at immediate, 1, 4, 7, 10 and 5 time periods.
The results of the study:
1, the CK value and LDH value of 1,4,7,10 days in the immediate and repair stage of the large load non treatment training group were higher than those in the quiet control group (P0.05). Compared with the medium load non treatment group, the CK value and LDH value of the non treatment group of large load were higher immediately after the injury and the first and fourth days after injury (P0.05), while the seventh to 10 days after the injury were large. The CK value and LDH value of the group were low, but there was no significant difference between the two (P0.05). Compared with the non load non treatment group, the LDH value of the rats in the non treatment training group was higher, but there was no significant difference between them (P0.05), and the CK value and LDH value were higher at the fourth day of the injury repair period (P0.05).
2, the CK value of the large load treatment group was lower than the non treatment group (P0.05) in the fourth day to 10 day after the skeletal muscle injury, while the LDH value was lower than the non treatment group (P0.05) at fourth days. Compared with the medium load treatment group and the non load treatment group, the CK value and the LDH value were higher at 4 days after the injury (P0.05).
3, the serum CK and LDH values were higher than those in the quiet control group (P0.05) on the first day after injury, and the serum CK and LDH values after the injury were close to the CK value of the quiet control group after the injury, and there was no significant difference between the two groups (P0.05).
4, the serum CK value was higher than that in the quiet control group (P0.05) on the 4 day and 7 days after the skeletal muscle injury in the non load non treatment group, and the CK enzyme value was close to the quiet control group on the tenth day after the injury, while the serum LDH was higher than that in the quiet control group (P0.05) after the skeletal muscle injury immediately and after the injury, and 4 days after the injury. The serum LDH was higher than the medium negative group (P0.05). The CK and LDH values in the non treatment group of the non load group were higher than those in the non load group (P0.05), and there was no significant difference between the first days and the tenth days after the injury (P0.05).
5, the CK value and LDH value of the fourth day to 10 day after skeletal muscle injury in the non load treatment group were lower than the non treatment group (P0.05) in the large load group, and higher than the medium intensity treatment training group for fourth days after the injury, but there was no significant difference (P0.05), and the CK value and LDH value of the non load group from 7 to 10 days were close to the quiet control group.
Conclusion:
1. according to the medium load non treatment group, the rats in the non treatment group had CK and LDH load and no load training in the non treatment group. There was no significant difference between the 4 days (P0.05), seventh days and tenth days after injury. It showed that the injury degree of the rats in the medium load group was lighter than the heavy load and the non load training group, and the natural repair was faster. It indicates that the injury resistance of skeletal muscle in middle load group is higher than that in high load group and no load group.
2. the LDH value of rat CK based on heavy load training was higher than that of the non load training rats immediately after injury, but there was no significant difference between them. In the 4 to 10 days after injury, the CK of the rats without load training was higher (P0.01), and we could think that the damage degree of the heavy load training group and the non load group was not immediately damaged. There were statistical differences, but the rate of natural repair in the large load training group was slower than that in the non load training group after 4 days of injury. It showed that the heavy load group could have repeated injury during the 8 week heavy load training and the induction of skeletal muscle injury, indicating the accumulation effect of skeletal muscle injury.
3. according to the large load group, the CK enzyme and LDH enzyme were lower than the non treatment group (P0.05) on the fourth day after the treatment of the skeletal muscle injury of the rats in the medium load group and the non load exercise group after the treatment of the Yunnan Baiyao, and the tenth day was lower than that of the non treatment group. It showed that the white medicine can accelerate the repair of the motor skeletal muscle injury in the rats.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R87

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