长期运动应激对大鼠肠道免疫机能的影响和益生菌的干预效果

发布时间：2018-05-14 10:37

本文选题：运动应激 + 肠道免疫　；参考：《中国运动医学杂志》2016年09期

【摘要】：目的:探索长期运动应激引起的肠道免疫机能变化机制以及益生菌的干预效果。方法:实验大鼠随机分为安静对照组、运动对照组、安静营养组、运动营养组,运动对照组和运动营养组大鼠进行每周6次、共9周的力竭性跑台训练。安静营养组和运动营养组每日灌胃新鲜配制的浓度为107CFU/m L鼠李糖乳杆菌溶液2 ml,9周后观察血清睾酮(T)、皮质醇(CORT)、促肾上腺皮质激素(ACTH)、脂多糖(LPS),以及小肠组织中白细胞介素-8(IL-8)、分泌型免疫球蛋白(s Ig A)含量。结果:与安静对照组和安静营养组相比,运动对照组和运动营养组血清ACTH均显著升高(P0.01),小肠组织中s Ig A与IL-8显著升高(P0.01;P0.05);血液中LPS水平运动对照组显著高于运动营养组(P0.05),但安静营养组显著高于安静对照组(P0.01)和运动营养组(P0.05)。结论:长期大强度运动引起大鼠应激激素的分泌增加,进而诱发小肠组织免疫应答被激活,炎症反应增强;单纯补充鼠李糖乳杆菌未能有效改善长期运动应激造成的内分泌及肠道机能下降,益生菌营养需要从菌群平衡的角度来辩证考虑。
[Abstract]:Objective: to explore the mechanism of intestinal immune function change induced by long-term exercise stress and the intervention effect of probiotics. Methods: the experimental rats were randomly divided into the quiet control group, the exercise control group, the quiet nutrition group, the exercise control group and the exercise nutrition group. 107CFU/m L Lactobacillus rhamnoides solution 2 ml / 9 weeks later, serum testosterone, cortisone, adrenocorticotropic hormone (ACTH), lipopolysaccharide (LPS), and small intestinal white tissue were observed. The content of interleukin-8 (IL-8) and secretory immunoglobulin (Ig A). Results: compared with quiet control group and quiet nutrition group, The levels of serum ACTH in exercise control group and exercise nutrition group were significantly higher than that in exercise nutrition group, while the levels of s Ig A and IL-8 in small intestine tissue were significantly higher than that in exercise nutrition group, while the level of LPS in blood in exercise control group was significantly higher than that in exercise nutrition group, but it was significantly higher in quiet nutrition group than that in quiet nutrition group. P0.01) and exercise nutrition group (P0.05). Conclusion: Long-term high intensity exercise can induce the increase of stress hormone secretion in rats, and then induce the activation of immune response in small intestine and the enhancement of inflammatory reaction. Lactobacillus rhamnoides supplementation alone can not effectively improve the endocrine and intestinal dysfunction caused by long-term exercise stress. The nutrition of probiotics should be considered dialectically from the point of view of flora balance.
【作者单位】：北京体育大学运动人体科学学院;
【基金】：北京体育大学校自主课题(2016BS003)
【分类号】：R87

【相似文献】