肿节风配方颗粒对小型猪腮腺放射防护作用的研究

发布时间：2018-05-15 17:44

  本文选题：肿节风 + 小型猪　； 参考：《广西医科大学》2013年硕士论文

【摘要】：目的：观察分割照射条件下,肿节风对腮腺照射后活性氧簇(ROS)的清除作用及对腮腺细胞凋亡的影响,探讨分割照射下肿节风对腮腺的防护作用。 方法：(1)实验对象为实验用巴马小型猪,建立起肿节风干预下的腮腺放射性损伤实验动物模型。60头小型猪随机分成空白对照组(对照组)、单纯照射组(单照组)和肿节风加照射组(药照组)3组,每组平行分成a、b、c、d四个亚组,分别于照射结束后1d、10d、40d和90d取腮腺组织,药照组于照射前一周开始给予肿节风颗粒,直至腮腺组织取出,对照组与单照组给予等量生理盐水。在全麻状态下药照组跟单照组给予总量为30Gy/(5f·5w)60C0γ射线双侧腮腺照射,对照组不予照射。(2)用猪活性氧(ROS)酶联免疫分析试剂盒检测其照射后1d、10d、40d、90dROS的含量及RT-PCR法检测照射后1d、10d、40d、90d腮腺细胞中Bcl-2、Bax、P53和Caspase-3mRNA表达量的变化：结果：(1)照射后1d,对照组、单照组、药照组ROS含量分别为62.58,136.75,62.83U/ml,对照组与药照组差异无统计学意义(P0.01),单照组与药照组、对照组之间差异有统计学意义(P0.01)；照射后10d,对照组、单照组、药照组ROS含量分别为64.50,338.5,281.67U/m1,照射后40d,对照组、单照组、药照组ROS含量分别为62.92,392.83,324.42U/m1,照射后90d,对照组、单照组、药照组ROS含量分别为62.75,438.92,323.75U/ml,每个时间段三组之间差异均有统计学意义(P0.01),药照组的ROS含量均低于单照组；(2)在同一时点(同一平行组),bc1-2mRNA表达量为空白组(0.84±0.05,0.89±0.09,0.85±0.06,0.92±0.04)药照组(0.52±0.09,0.58±0.06,0.63±0.02,0.69±0.07)单照组(0.47±0.07,0.23±0.05,0.31±0.06,0.39±0.09):baxmRNA表达量为单照组(0.92±0.09,1.57±0.05,1.41±0.08,1.33±0.08)药照组(0.78±0.08,0.69±0.08,0.62±0.07,0.59±0.04)空白组(0.56±0.04,0.48±0.08,0.55±0.09,0.49±0.06)；p53mRNA表达量为单照组(1.55±0.21,2.34±0.36,3.8±0.39,4.0±0.48)药照组(1.05±0.19,1.86±0.31,2.67±0.36,3.14±0.27)空白组(0.67±0.15,O.73±0.12,0.62±0.37,0.55±0.29)；Caspase-3mRNA表达量为单照组(0.54±0.14,0.62±0.09,0.96±0.17,1.17±0.21)药照组(0.45±0.06,0.57±0.09,0.84±0.12,0.99±0.15)空白组(0.40±0.06,0.37±0.09,0.29±0.04,0.30±0.05)；bcl-2mRNA表达量在照射结束后10d最低、90d最高；bax蛋白在10d最高、90d最低；药照组中,bcl-2mRNA表达量逐渐递增；baxmRNA表达量逐渐递减(P0.05)。单照组中p53和Caspase-3mRNA的表达量均呈逐渐增高趋势,药照组中,p53及Caspase-3mRNA的表达量也呈现逐步升高趋势,但各时间段表达量均低于单照组(P0.05) 结论：(1)分割照射下,成功建立起肿节风干预下的腮腺放射性损伤实验动物模型。(2)肿节风通过清除小型猪腮腺放射后的ROS活性,能有效减缓腮腺放射损伤。(3)肿节风抑制腮腺细胞放疗后细胞凋亡的作用,可能是通过抑制Bax、P53和Caspase3mRNA的表达,同时上调Bcl-2mRNA的表达来实现。
[Abstract]:Aim: to observe the scavenging effect of Jiefeng on rosy after parotid gland irradiation and its effect on apoptosis of parotid gland cells, and to explore the protective effect of Tetrandra on parotid gland under fractionated irradiation. Methods Bama miniature pig was used as experimental object. 60 miniature pigs were randomly divided into three groups: control group (control group), simple irradiation group (single irradiation group), and swelling section plus irradiation group (drug irradiation group), and 60 miniature pigs were randomly divided into three groups: control group (control group), single irradiation group (single irradiation group) and combined irradiation group (drug irradiation group). Each group was divided into four subgroups in parallel. Parotid tissues were taken at 10 days after irradiation for 40 days and 90 days after irradiation. The control group was given the same amount of normal saline one week before irradiation until the parotid gland tissue was taken out, and the control group and the single irradiation group were given the same amount of normal saline. Under general anesthesia, the total amount of 30Gy/(5f 5w)60C0 纬 -ray irradiation on both sides of parotid gland was given to the drug irradiation group and the single irradiation group. In the control group, the Ros content was detected by enzyme linked immunosorbent assay (Elisa) kit of porcine reactive oxygen species (Ros) and the changes of Bcl-2OBX BaxP53 and Caspase-3mRNA expression in parotid gland cells were detected by RT-PCR assay at 1 day, 10 days after irradiation, 40 days after irradiation and 90 days after irradiation: results one day after irradiation, control group, single irradiation group, and the control group, single irradiation group, the expression of Bcl-2P BaxP53 and BaxP53 in parotid gland cells were determined. The content of ROS was 62.58136.75U / ml in the control group and 62.83U / ml in the control group, respectively. There was no significant difference between the control group and the drug exposure group (P 0.01), but the difference between the single irradiation group and the medicine irradiation group was statistically significant (P 0.01), 10 days after irradiation, the control group, the single irradiation group, the control group and the control group. The ROS content of the drug irradiation group was 64.50338.5n 281.67 U / m 1. The ROS content of the control group, the single irradiation group and the drug irradiation group was 62.92392.83 ~ 324.42 U / m ~ (-1) at 40 days after irradiation, respectively. 90 days after irradiation, the control group, the single irradiation group, the control group, the single irradiation group, The ROS content of the drug exposure group was 62.75438.92 ~ 323.75 U / ml, the difference between the three groups was statistically significant in each time period, and the ROS content in the drug irradiation group was lower than that in the single irradiation group (the same parallel group was 0.84 卤0.050.89 卤0.069 卤0.92 卤0.92 卤0.92) at the same time point (0.52 卤0.09 卤0.58 卤0.06 卤0.63 卤0.020.69 卤0.07) at the same time point (0.84 卤0.050.89 卤0.069 卤0.92 卤0.92 卤0.92 卤0.92 卤0.06 卤0.06 卤0.06 卤0.06 卤0.69 卤0.07 in the same parallel group, respectively) in the same time point (0. 84 卤0. 05 卤0. 05 卤0. 05 卤0. 92 卤0. 92 卤0. 92 卤0. 92). (0.47卤0.07,0.23卤0.05,0.31卤0.06,0.39卤0.09):baxmRNA琛ㄨ揪閲忎负鍗曠収缁，

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