急性运动对小鼠骨骼肌核蛋白Nrf2-ARE结合活性的影响

发布时间：2018-05-16 11:23

本文选题：Nrf-ARE结合活性 + 核蛋白　；参考：《中国运动医学杂志》2015年07期

【摘要】：目的:通过一次性不同时间的急性运动,探讨小鼠骨骼肌中核因子E2相关因子2(Nrf2)与抗氧化反应元件(ARE)结合活性随运动时间的变化。方法:将C57BL/6J野生小鼠,根据运动时间随机分为三组,分别是安静对照组(0h组)、1小时运动组(1h组)和6小时运动组(6h组);采取坡度为5%,速度为20m/min的跑台运动,运动结束后3小时取两侧骨骼肌。酶联免疫法(ELISA)测定骨骼肌细胞核中的Nrf2-ARE结合活性,Western Blot法测定骨骼肌核蛋白中Nrf2含量,实时荧光定量PCR测超氧化物歧化酶1(SOD1)、超氧化物歧化酶2(SOD2)、谷胱甘肽还原酶(GR)、γ谷氨酰半胱氨酸连接酶(GCLm)、谷胱甘肽过氧化物酶(Gpx1)和血红素加氧酶(HO-1)等基因的m RNA表达水平。结果:(1)Nrf2的结合活性1 h组与0 h组相比基本无变化,6 h组与0 h组相比显著性增加(P0.05);(2)核蛋白Nrf2的相对含量1 h组与0 h组相比基本无变化,6 h组与0 h组相比有增加的趋势但无显著性;(3)随着运动时间的增加,小鼠骨骼肌中GCLm、SOD1、SOD2、HO-1 m RNA相对表达量逐渐增高;Gpx1 m RNA相对表达量有增加的趋势但是无显著性;GR m RNA相对表达量无明显变化;与安静对照组相比,6 h组GCLm(P0.01)、SOD1(P0.01)、SOD2(P0.05)、HO-1(P0.01)m RNA相对表达量非常显著性或显著性增加。结论:(1)骨骼肌核蛋白Nrf2-ARE结合活性与运动时间呈正相关;(2)一次6小时长时间急性运动促进骨骼肌中Nrf2蛋白的核转位,显著增加核蛋白Nrf2-ARE的结合活性以及抗氧化酶GCLm、SOD1、SOD2、HO-1、Gpx1基因的m RNA水平。
[Abstract]:Aim: to investigate the effect of exercise time on the binding activity of nuclear factor E2 related factor 2nrf2 to antioxidation response element (ARE) in skeletal muscle of mice by acute exercise at different time. Methods: the wild C57BL/6J mice were randomly divided into three groups according to their exercise time. They were divided into three groups: the quiet control group (0 h) and the 6-hour exercise group (1 h) and the 6-hour exercise group (6 h). The skeletal muscles of both sides were taken 3 hours after exercise. Enzyme linked immunosorbent assay (Elisa) for the determination of Nrf2-ARE binding activity in skeletal muscle nuclei. Western Blot assay was used to determine the content of Nrf2 in skeletal muscle nuclear protein. The expression levels of m RNA of superoxide dismutase (1), superoxide dismutase (2), glutathione reductase (GRN), 纬 glutamyl cysteine ligase (GCLm1), glutathione peroxidase (Gpx1) and heme oxygenase (HO-1) were measured by real-time fluorescence quantitative PCR. Results compared with 0 h group, the binding activity of Nrf2 in the 1 h group was significantly higher than that in 0 h group. The relative content of nuclear protein Nrf2 in the 1 h group was significantly higher than that in the 0 h group (P 0. 05) the relative content of nucleoprotein Nrf2 in the 1 h group was almost unchanged compared with the 0 h group and the 0 h group was significantly higher than that in the 0 h group and the 6 h group was significantly higher than the 0 h group. The increasing trend is not significant with the increase of exercise time, The relative expression of HO-1 m RNA in skeletal muscle of mice was gradually increased, but there was no significant change in the relative expression of gr m RNA. Compared with the quiet control group, the relative expression of RNA in the 6 h group was significantly or significantly higher than that in the control group. Conclusion (1) Nrf2-ARE binding activity of skeletal muscle nuclear protein is positively correlated with exercise time. (2) Acute exercise for 6 hours can promote the nuclear translocation of Nrf2 protein in skeletal muscle. The binding activity of nucleoprotein Nrf2-ARE and the level of m RNA of antioxidation enzyme GCLm1 / SOD2 / HO-1Gpx1 gene were significantly increased.
【作者单位】：北京体育大学运动人体科学学院;
【基金】：国家自然科学基金(31471134) 国家体育总局科研课题(2014B010)
【分类号】：R87

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