髌骨髌腱结合部跳跃损伤动物模型建立及运动后冷疗对损伤的影响

发布时间：2018-05-17 21:46

  本文选题：髌骨髌腱结合部 + 跳跃运动　； 参考：《北京体育大学》2015年博士论文

【摘要】：目的：建立髌骨髌腱结合部跳跃损伤动物模型和离心运动动物模型,分别用于研究长期和急性跳跃运动对髌骨髌腱结合部(PPTJ)组织结构、细胞外基质和细胞因子的影响,并探讨运动后冷疗的作用。方法：实验一：18周龄新西兰大白兔分为2周组、4周组和对照组,采用兔跳跃装置进行跳跃训练,每天跳跃150次,每周连续5天。HE、Safranin o染色显示组织学变化,免疫组化染色显示血管生成、炎性反应、损伤修复等相关因子的表达。实验二：分组为运动组、冷疗组和对照组,跳跃运动方案同实验一,观察运动后冷疗对PPTJ组织损伤及肌肉物理特性的影响。实验三：分组为急性运动组和运动后冷疗组和对照组,采用兔离心运动模型进行200次急性落地运动,观察运动后及运动后冷疗2h、24h、48h组织学变化及促炎性因子和生长因子的表达,并观察运动后冷疗对肌肉物理特性的影响。结果：实验一：与对照组相比,4周组局部出现胶原纤维排列紊乱、细胞和粘多糖蛋白分布不均等改变,细胞密度显著增加(p0.05)、纤维软骨带增厚(p0.01),ColⅢ显著增加(p0.01),ColⅢ/ColⅠ比值显著增加。IL-6、COX-2、 HIF-1α、VEGF、TGF-β2、BMP、Caspase3阳性染色细胞密度显著增高(P0.01),SP表达增高(P0.05)。实验二：运动组和冷疗组组织形态学指标及胶原蛋白表达无明显差异,但冷疗组IL-6和Cox-2表达低于运动组,运动后冷疗在第1周可以显著降低肌肉硬度和肌肉张力。实验三：急性运动组组织学指标未见显著改变,IL-6、COX-2在运动后2h达到峰值,Col Ⅰ、ColⅢ/TGF-β2阳性表达在24h时达到峰值,bFGF在48h时达到峰值,均显著高于对照组(P0.01)。运动后冷疗组在运动后2h时COX-2和工L-6表达显著低于急性运动组,各个时间点上肌肉硬度显著低于急性运动组(P0.05)。结论；4周跳跃运动可建立PPTJ跳跃损伤动物模型,缺氧及血管生成、炎性反应、Ⅲ胶原表达增高、细胞凋亡增加是PPTJ过度使用性损伤的重要病理特点。运动后冷疗对PPTJ损伤发生未显示干预作用。急性或长期运动后冷疗可降低炎性反应,缓解疼痛,并具有一定的降低肌肉硬度作用。
[Abstract]:Objective: to establish the animal models of patellar tendon joint injury and eccentric exercise, and to study the effects of long-term and acute jumping exercise on the tissue structure, extracellular matrix and cytokines of patella patellar tendon. The effect of cold therapy after exercise was discussed. Methods: new Zealand white rabbits at the age of 18 weeks were divided into 2 week group and 4 week group and control group. The jumping training was carried out with rabbit jumping device. The jumping time was 150 times a day. The histopathological changes were observed by Safranin o staining for 5 days a week. Immunohistochemical staining showed the expression of angiogenesis, inflammatory response, injury repair and other related factors. Experiment 2: divided into exercise group, cold therapy group and control group. The jumping exercise scheme was the same as experiment 1. The effects of cold therapy after exercise on PPTJ tissue injury and muscle physical characteristics were observed. Experiment 3: acute exercise group, cold therapy group and control group were divided into acute exercise group, cold therapy group and control group. Two hundred times of acute landing exercise were performed with rabbit centrifugal exercise model. The histological changes and the expression of pro-inflammatory and growth factors were observed at 24 h after exercise and 2 h after cold therapy. The effects of cold therapy after exercise on the physical properties of muscles were observed. Results: experiment 1: compared with the control group, the collagenous fibers were disordered and the distribution of cell and mucopolysaccharide protein was not equal in the 4th week group. The cell density was significantly increased, and the ratio of p0.01Col 鈪，

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