电离辐射对小鼠骨髓c-kit阳性细胞损伤效应体外研究

发布时间：2018-05-25 18:45

本文选题：骨髓 + 辐射　；参考：《重庆医学》2015年24期

【摘要】：目的观察电离辐射引起小鼠骨髓c-kit+细胞损伤变化。方法磁珠法分选小鼠c-kit+细胞,生物发光法检测细胞活力,活性氧探针(DCFH-DA)检测细胞内活性氧水平,集落形成数目检测克隆形成能力,流式细胞术检测细胞增殖与凋亡,基因芯片分析细胞内信号通路变化。结果与0Gy组(对照组)比较,c-kit+细胞受到1Gy和4Gy照射后,细胞活力下降,克隆形成能力下降,早期凋亡和晚期凋亡细胞比例增加,细胞内活性氧水平升高;与对照组比较,c-kit+细胞受到1Gy照射后,处于增殖期(S/G2/M期)细胞比例增加;4Gy照射后处于增殖期(S/G2/M期)细胞比例下降;c-kit+细胞受到4Gy照射后Srxn1、Psmb5、Cdkn1a、Smc1b、Bcl2l1、Lrdd等基因表达上调;Mpo、Mtf1、Chek1、Rcc1Ebag9、Ciapin1等基因表达下调。结论电离辐射能够引起骨髓c-kit+细胞损伤,导致一系列信号通路表达变化。
[Abstract]:Objective to observe the damage of bone marrow c-kit cells induced by ionizing radiation. Methods Mouse c-kit cells were selected by magnetic bead method, cell viability was detected by bioluminescence assay, reactive oxygen species (Ros) was detected by DCFH-DAA, colony formation was detected by colony forming number, cell proliferation and apoptosis were detected by flow cytometry. The changes of intracellular signaling pathway were analyzed by microarray. Results compared with 0Gy group (control group), c-kit cells were exposed to 1Gy and 4Gy, the cell viability decreased, clone formation ability decreased, the proportion of early and late apoptotic cells increased, and the level of reactive oxygen species increased. Compared with the control group, the proportion of c-kit cells in the proliferative phase of S / G _ 2 / M) increased after 4 Gy irradiation. The percentage of c-kit cells in the proliferative phase of S / G _ 2 / M) decreased. After 4Gy irradiation, the expression of genes such as Srxn1psmb5, Cdknn1psmbc1bBcl2l1and Lrdd up-regulated the expression of genes such as MpoMtf1, Chek1Rcc1Ebag9, Ciapin1 and so on. Conclusion Ionizing radiation can damage bone marrow c-kit cells and change the expression of a series of signal pathways.
【作者单位】：中国医学科学院放射医学研究所/天津市放射医学与分子核医学重点实验室;
【基金】：国家自然科学基金青年项目(81402633) 协和青年基金资助和中央高校基本科研业务费专项资金资助项目(33320140033)
【分类号】：R818

【共引文献】