不同运动方式对大鼠血清与肝脏中hepcidin水平及相关调节因素的影响

发布时间：2018-05-25 22:24

本文选题：铁调素 + 铁代谢　；参考：《中国运动医学杂志》2017年07期

【摘要】：目的:比较耐力运动及力竭运动对大鼠血液及肝脏中铁调素(hepcidin)表达的影响,并测定分析与之相关的铁代谢指标、低氧应答因子、炎性因子、抗菌肽活性相关指标的变化趋势。方法:雌性SD大鼠30只,随机分为对照组(n=10)、耐力运动组(n=10)、力竭运动组(n=10),对照组不运动,耐力运动组和力竭运动组分别采用为期4周的中等运动强度跑台训练和力竭运动强度跑台训练对大鼠进行干预,而后分别采集血样,进行血常规指标及血清中铁转运因子、低氧应答因子、炎性因子及抗菌肽活性相关指标测定;取肝脏标本,固定、包埋、切片后,利用免疫组织化学染色分别测定不同组别动物肝脏中hepcidin、低氧诱导因子(HIF-1α)、核转录因子kappa b(NF-κB)含量。结果:与对照组相比,4周耐力性运动后大鼠血清中hepcidin水平降低(P0.05),促红细胞生成素(EPO)浓度升高(P0.05),白细胞介素6(IL-6)浓度下降(P0.01)。与对照组相比,4周力竭性运动后大鼠血清及肝脏中hepcidin水平升高(P0.01),EPO浓度降低(P0.01),同时伴有血清及肝脏中HIF-1α表达增加(P0.01),血清转铁蛋白(TF)、可溶性转铁蛋白受体(s TFR)水平降低(P0.01),血清中白细胞介素1(IL-1)、IL-6、肿瘤坏死因子α(TNF-α)、C反应蛋白(CRP)水平显著升高(P0.01,P0.001,P0.001,P0.001);同时血清中与hepcidin抗菌肽活性相关的toll样受体4(TLR4)、NF-κB水平均显著升高(P0.001)。结论:4周耐力性运动可下调大鼠血清中hepcidin水平;而4周力竭性运动可使大鼠血清及肝脏中hepcidin水平显著上调,提示不同运动方式对hepcidin的影响存在明显差异。
[Abstract]:Aim: to compare the effects of endurance exercise and exhaustive exercise on the expression of heparin hepcidin in blood and liver of rats, and to determine the related indexes of iron metabolism, hypoxia response factor, inflammatory factor and antibacterial peptide activity. Methods: thirty female Sprague-Dawley rats were randomly divided into control group (n = 10), endurance exercise group (n = 10), exhaustive exercise group (n = 10) and control group (n = 10). Rats in endurance exercise group and exhaustive exercise group were treated with moderate intensity treadmill training and exhaustive exercise intensity treadmill training for 4 weeks respectively. Blood samples were collected and blood routine indexes and iron transport factors in serum were collected. The activity of hypoxia response factor, inflammatory factor and antimicrobial peptide were measured. The contents of hepcidin, hypoxia-inducible factor (HIF-1 伪) and nuclear transcription factor (kappa) NF- 魏 B in the liver of different groups of animals were determined by immunohistochemical staining. Results: compared with the control group, the levels of serum hepcidin, erythropoietin (EPO), interleukin-6 (IL-6) and interleukin-6 (IL-6) decreased in rats after 4 weeks of endurance exercise. Compared with the control group, the level of hepcidin in serum and liver of rats after 4 weeks exhaustive exercise was higher than that of the control group. The concentration of P0.01a was decreased, the expression of HIF-1 伪 in serum and liver was increased, and the levels of serum transferrin, transferrin receptor and soluble transferrin receptor were decreased. The levels of IL-6, TNF- 伪 -TNF- 伪 -C-reactive protein (CRP-) in serum were significantly higher than those in control group, and the levels of toll receptor 4TLR4NF-魏 B related to the activity of hepcidin antimicrobial peptide in serum were significantly higher than that of P0.001, and the levels of toll receptor 4TLR4NF-魏 B in serum were significantly higher than those in the control group (P < 0.05), while the levels of TNF- 魏 B in serum were significantly higher than those in the control group (P < 0.05). Conclusion the level of hepcidin in serum can be down-regulated by endurance exercise for 4 weeks, and the level of hepcidin in serum and liver can be upregulated by exhaustive exercise at 4 weeks, indicating that there are significant differences in the effect of different exercise modes on hepcidin.
【作者单位】：山东体育学院运动与健康学院;军事医学科学院放射与辐射医学研究所;
【基金】：山东省自然科学基金(ZR2014JL024) 山东省高等学校科技计划项目(J13LE04) 国家重点基础研究发展计划资助项目子课题(2012CB518206)
【分类号】：R87

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