MAOA μ-VNTR多态性与云南汉族、彝族躯体攻击行为相关性研究

发布时间：2018-05-26 12:17

本文选题：MAOA + μ-VNTR　；参考：《昆明医科大学》2017年硕士论文

【摘要】：研究目的建立MAOA μ-VNTR态简便、快速、准确的分型方法;探讨单胺氧化酶A基因(Monoamine oxidase-A,MAO-A)启动子区域30bp-VNTR(MAOA μ-VNTR)多态位点与云南汉族、彝族人群躯体攻击行为的相关性,揭示攻击行为遗传机制,为预防控制攻击行为提供理论依据。研究方法应用荧光标记引物扩增结合毛细管电泳建立M4OAμ-VNTR多态位点自动分型技术,并与传统的非标记引物扩增结合琼脂糖凝胶电泳检测方法比较;应用荧光标记毛细管电泳技术,对495例男性躯体攻击行为者(云南汉族315例、彝族180例)和527例男性健康对照(云南汉族366例、彝族161例)进行MAOA μ-VNTR基因分型。运用SPSS19.0软件进行数据统计。研究结果1.AAOA μ-NTR自动分型方法建立了基于荧光标记引物毛细管电泳检测的自动分型方法,简便快速,准确高效,分型结果与传统的琼脂糖凝胶检测法一致。2.流行病学分析结果具有躯体攻击行为者的平均年龄为29.16±9.41岁,其中18～40岁的患者最多,在云南汉族和彝族群体中初中及以下低文化程度群体(P=O.001,OR1)躯体攻击行为风险增加;未婚或离婚群体、吸烟群体、饮酒群体发生躯体攻击行为的风险高于在婚群体、不吸烟群体、不饮酒群体(OR1),但均无统计学意义(P0.05)。3.M40A μ-VNTR多态性分布在云南汉族、彝族群体中只观察到3R、4R等位基因。在云南汉族群体,3R频率为0.548、4R频率为0.452;在云南彝族群体中,3R频率为0.478、4R频率为0.522;云南汉族、彝族MAOA μ-VNTR分布具有显著性差异。4.MAOA μ-VNTR多态性与躯体攻击行为的相关性在云南汉族群体中MAOA μ-VNTR多态在攻击组和非攻击组中的分布无显著性差异(P=0.703)。在云南彝族群体中M4OA μ-VNTR多态在攻击组和非攻击组中的分布无显著性差异(P=0.835)。5.MAOA μ-VNTR多态性与潜在风险因素交互作用分析结果在云南汉族人群中将文化程度、婚姻状况、饮酒和吸烟四个风险因素分别与MAOAμ-VNTR进行交互分析,均未发现四个风险因素与MAOA μ-VNTR存在交互作用(P0.05),但是Logistic回归分析发现低文化程度和吸烟史是躯体攻击行为的风险因素。在云南彝族人群中将文化程度、婚姻状况、饮酒和吸烟四个风险因素分别与MAOAμ-VNTR进行交互分析,均未发现四个风险因素与MAOA μ-VNTR存在交互作用(P0.05),但是Logistic回归分析发现低文化程度和吸烟史是躯体攻击行为的风险因素。研究结论1、本课题建立了MAOA μ-VNTR多态性自动分型方法,具有高效、简便、快速、准确的优点。2、云南汉族、彝族的MAOA μ-VNTR多态位点与躯体攻击行为无显著关联。3、在云南汉族、彝族群体中低文化程度和吸烟史是躯体攻击行为的风险因素。
[Abstract]:Objective to establish a simple, rapid and accurate method for the typing of MAOA 渭 -VNTR, and to investigate the relationship between 30bp-VNTR(MAOA 渭 -VNTRR polymorphism in Monoamine oxidase-A (MAO-A) promoter region and somatic aggression behavior in Yunnan Han and Yi people. To reveal the genetic mechanism of attack behavior and to provide theoretical basis for preventing and controlling attack behavior. Methods M4OA 渭 -VNTR polymorphic locus typing technique was established by fluorescence labeled primer amplification combined with capillary electrophoresis, and compared with the traditional non-labeled primer amplification method combined with agarose gel electrophoresis, and the fluorescence labeled capillary electrophoresis technique was used to detect the polymorphic loci of M4OA 渭 -VNTR. The MAOA 渭 -VNTR genotyping was performed in 495 male somatic aggression behaviors (315 Han, 180 Yi) and 527 healthy controls (366 Han and 161 Yi). Use SPSS19.0 software to carry on data statistics. Results 1.AAOA 渭 -NTR automatic typing method based on fluorescence labeled primer capillary electrophoresis was established. The method was simple, rapid, accurate and efficient. The typing results were consistent with the traditional agarose gel detection method. The results of epidemiological analysis showed that the average age of the subjects with physical aggression was 29.16 卤9.41 years old, the most of them were 1840 years old, and the risk of somatic aggression was increased in the lower education groups of Han and Yi nationality in Yunnan province. The risk of somatic aggression in unmarried or divorced population, smoking group and drinking group was higher than that in married group, non-smoking group and non-drinking group, but there was no statistically significant distribution of P0.05A .3.M40A 渭 -VNTR polymorphism in Yunnan Han nationality. Only 3R 4R allele was observed in Yi population. In Yunnan Han population, the frequency of 3R is 0.548R, the frequency of 4R is 0.452, and the frequency of 3R is 0.522 in Yunnan Yi population, the frequency of Han nationality in Yunnan is 0.522, the frequency of 3R is 0.522, the frequency of 4R is 0.522. There was significant difference in the distribution of MAOA 渭 -VNTR in Yi nationality. 4. There was no significant difference in the distribution of MAOA 渭 -VNTR polymorphism between attack group and non-attack group in Yunnan Han population. 4. There was no significant difference between MAOA 渭 -VNTR polymorphism and somatic aggression behavior in Yunnan Han population. There was no significant difference in the distribution of M4OA 渭 -VNTR polymorphism between the attack group and the non-aggressive group in the Yi ethnic group of Yunnan. The results of interaction analysis between the polymorphism of M4OA 渭 -VNTR and the potential risk factors in Yunnan Han population were as follows: the educational level and marital status of the Han nationality in Yunnan were analyzed. Four risk factors of alcohol consumption and smoking were interacted with MAOA 渭 -VNTR. There was no interaction between four risk factors and MAOA 渭 -VNTR. However, Logistic regression analysis showed that low education level and smoking history were risk factors of somatic aggression behavior. Four risk factors, including education, marital status, alcohol consumption and smoking, were interacted with MAOA 渭 -VNTR in Yunnan Yi population. There was no interaction between four risk factors and MAOA 渭 -VNTR, but Logistic regression analysis showed that low education level and smoking history were risk factors of somatic aggression behavior. Conclusion 1. The MAOA 渭 -VNTR polymorphism automatic typing method was established in this study. It has the advantages of high efficiency, simplicity, rapidity and accuracy. There is no significant correlation between MAOA 渭 -VNTR polymorphism locus and somatic aggression behavior in Yunnan Han and Yi nationality, but there is no significant correlation between the polymorphism of MAOA 渭 -VNTR polymorphism and somatic aggression behavior in Yunnan Han nationality. Low education level and smoking history are risk factors of physical aggression in Yi nationality.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：D919.4

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