力竭运动对大鼠前额叶皮层小清蛋白阳性神经元及NMDAR2B表达的影响

发布时间：2018-05-26 22:24

本文选题：运动疲劳 + NMDARB　；参考：《中国运动医学杂志》2017年03期

【摘要】：目的:通过观察力竭运动后大鼠前额叶皮层小清蛋白(parvalbumin,PV)阳性神经元的表达变化,探讨运动疲劳对前额叶皮层神经微环路可塑性的影响,同时观察前额叶皮层N-甲基-D-天门冬氨酸受体NR2B亚型(N-methyl-D-aspartatereceptor NR2B subunit,NMDAR2B)表达的变化,进一步探讨运动疲劳中枢调节的可能机制。方法:雄性Wistar大鼠36只随机分为一次力竭运动组、重复力竭运动组和对照组。力竭运动方案采用本实验室改进的Bedford渐增负荷动物运动方案:I级:8.2 m/min,15 min;II级:15 m/min,15 min;III级:20 m/min,至力竭,重复力竭运动组连续进行7天力竭性跑台运动,在运动力竭后采用免疫荧光染色技术观察大鼠前额叶皮层PV阳性神经元的表达情况,并记录阳性细胞个数。采用免疫荧光染色技术和免疫印迹法检测前额叶皮层NMDAR2B的表达情况。结果:一次力竭运动组及重复力竭运动组大鼠前额叶皮层PV阳性神经元个数较对照组显著增加(P0.01)。免疫荧光染色结果显示,前额叶皮层NMDAR2B阳性神经元在对照组和重复力竭运动组未见明显表达,一次力竭运动组可见阳性神经元表达;Western blot结果显示,与对照组相比,一次力竭运动组NMDAR2B表达相对较高,而重复力竭运动组表达相对较低,但均无显著性差异(P0.05);NMDAR2B表达与运动距离呈显著负相关(P0.01,r=-0.936)。结论:力竭运动通过PV阳性神经元的局部环路的调节影响大鼠前额叶皮层神经网络的可塑性,其参与了运动疲劳的中枢调节,为运动疲劳中枢机制研究提供了形态学基础。
[Abstract]:Objective: to investigate the effect of exercise fatigue on the plasticity of prefrontal cortex microloop by observing the expression of PV-positive neurons in prefrontal cortex of rats after exhaustive exercise. The expression of N-methyl-Daspartate receptor NR2B subtype (N-methyl-D-aspartate receptor NR2B subunit B) in prefrontal cortex was observed and the possible mechanism of central regulation of exercise fatigue was explored. Methods: 36 male Wistar rats were randomly divided into one exhaustive exercise group, repeated exhaustive exercise group and control group. The exhaustive exercise program was developed in our laboratory using the Bedford incremental animal exercise program: 1: I grade 8.2 m / min and 15 min class II: 15 m / min = 15 m / min = 20 m / min. The exhaustive exercise group performed exhaustive treadmill exercise for 7 consecutive days, and the exhaustive exercise group continued to perform exhaustive treadmill exercise for 7 days. The expression of PV-positive neurons in prefrontal cortex of rats was observed by immunofluorescence staining after exercise exhaustion, and the number of positive neurons was recorded. The expression of NMDAR2B in prefrontal cortex was detected by immunofluorescence staining and Western blotting. Results: compared with the control group, the number of PV positive neurons in the prefrontal cortex of the rats in the single exhaustive exercise group and the repeated exhaustive exercise group was significantly increased (P 0.01). The results of immunofluorescence staining showed that there was no significant expression of NMDAR2B positive neurons in prefrontal cortex in control group and repeated exhaustive exercise group. The expression of NMDAR2B was higher in one exhaustive exercise group than that in repeated exhaustive exercise group, but there was no significant difference in the expression of NMDAR2B between P0.05 and NMDAR2B. There was a significant negative correlation between the expression of NMDAR2B and the exercise distance. Conclusion: exhausted exercise affects the plasticity of prefrontal cortex neural network through the regulation of local loop of PV-positive neurons, which participates in the central regulation of exercise fatigue and provides a morphological basis for the study of the mechanism of exercise fatigue.
【作者单位】：北京师范大学体育与运动学院;北京市体育科学研究所;广州中医药大学;
【基金】：国家自然科学基金资助项目(31571221) 北京市自然科学基金资助项目(5142012)
【分类号】：R87

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