miR-21对运动性右室心肌纤维化发生的影响

发布时间：2018-05-29 12:51

本文选题：运动 + 右心室　；参考：《中国运动医学杂志》2017年07期

【摘要】：目的:研究在长期大强度运动引起的右室心肌纤维化中miR-21表达水平的变化,探讨miR-21在运动性心肌纤维化中的作用。方法:72只雄性SD大鼠,随机分为安静对照组(Sed,n=24)、中强度运动组(ME,n=24)和大强度运动组(IE,n=24),每组按照训练周期又分为8周、12周和16周,各8只。安静对照组自由活动,中强度组和大强度组分别以速度15.2 m/min、坡度5°和速度28 m/min、坡度10°的条件每天运动1 h,每周运动5天。各组最后一次运动结束24小时后,下腔静脉采血并处死,迅速分离心脏取右心室。天狼星红染色测定右心室的胶原容积分数(CVF);免疫荧光检测Ⅰ胶原蛋白(ColⅠ)的含量;RT-PCR检测miR-21的表达水平。结果:12周(P0.05,P0.05)和16周(P0.01,P0.01)大强度运动后大鼠右室心肌胶原容积分数显著高于安静对照组与中强度运动组;与安静对照组和中强度运动组比,12周(P0.01,P0.01)和16周(P0.01,P0.01)大强度运动使右室Ⅰ胶原蛋白的含量显著增多。与安静对照组相比,8周、12周和16周大强度运动都可使大鼠miR-21表达水平显著升高(P0.01、P0.05和P0.05),且在16周时miR-21的表达水平与Ⅰ胶原蛋白的含量正相关(r=0.443,P=0.03)。结论:长期大强度运动后右室心肌纤维化与miR-21表达水平增高有关,miR-21是潜在的运动性心肌纤维化的新生物标志物和干预靶点。
[Abstract]:Objective: To study the change of miR-21 expression level in right ventricular fibrosis induced by long term intense exercise and explore the role of miR-21 in motor myocardial fibrosis. Methods: 72 male SD rats were randomly divided into a quiet control group (Sed, n=24), a moderate intensity exercise group (ME, n=24) and a large intensity exercise group (IE, n=24). Each group was trained according to the training cycle. It was divided into 8 weeks, 12 weeks and 16 weeks, each 8. The control group was free, the medium strength group and the large strength group were 15.2 m/min, the slope 5 and 28 m/min, the gradient 10 degrees were 1 h every day and 5 days a week. After the final movement ended 24 hours, the inferior vena cava was collected and executed, and the right ventricle was quickly separated from the heart. Sirius red staining was used to determine the collagen volume fraction (CVF) of the right ventricle, the content of collagen (Col I) by immunofluorescence, and the expression level of miR-21 by RT-PCR. Results: the myocardial collagen volume fraction of right ventricular myocardium in 12 weeks (P0.05, P0.05) and 16 weeks (P0.01, P0.01) was significantly higher than that in the quiet control group and the middle strength exercise group. In the quiet control group and the moderate intensity exercise group, the 12 weeks (P0.01, P0.01) and the 16 weeks (P0.01, P0.01) increased the right ventricular I collagen content significantly. Compared with the quiet control group, the high intensity exercise of 8 weeks, 12 weeks and 16 weeks could significantly increase the level of miR-21 expression in rats (P0.01, P0.05 and P0.05), and the expression of water at miR-21 at the time of 16 weeks. There is a positive correlation between level and content of collagen I (r=0.443, P=0.03). Conclusion: the right ventricular myocardial fibrosis is associated with the increase of miR-21 expression after long term high intensity exercise. MiR-21 is a new biomarker and target target for potential exercise-induced myocardial fibrosis.
【作者单位】：国家体育总局体育科学研究所;上海体育学院运动科学学院;湖南工业大学体育学院;
【基金】：基金项目:国家体育总局体育科学研究所基本科研业务经费(基本15-37和16-21)
【分类号】：R87

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