黄精多糖对一次大强度运动后人体外周血T淋巴细胞免疫功能的影响

发布时间：2018-05-29 23:39

本文选题：黄精多糖 + 大强度运动　；参考：《成都体育学院》2012年硕士论文

【摘要】：目的：观察黄精多糖对大强度运动所致人体T淋巴细胞免疫功能低下的影响，分析其产生影响的可能机制，探讨减轻运动性免疫功能低下的新手段。方法：体育学院无系统专业运动训练经历男生14名，随机分为安慰剂组（A组）和黄精多糖组（B组），每组7人。A、B两组受试者按1g/d剂量分别口服安慰剂和黄精多糖4周后，进行一次大强度递增负荷跑台运动，于运动前、运动后即刻、运动后24h取受试者肘正中静脉血各10ml，MTT法检测外周血T淋巴细胞转化指数（T-LTI），流式细胞术检测T淋巴细胞亚群（CD4~+、CD8~+）百分比，双抗体夹心ABC—ELISA法检测Th1、Th2型细胞因子（IFN-γ、IL-4）、放射免疫法检测血浆皮质醇（Cor）。结果：1.A、B两组受试者运动前各指标无显著性差异。2.两组受试者T-LTI运动后即刻均低于运动前，其中A组有非常显著性差异；运动后24h，两组T-LTI均有回升，A组接近运动前水平，B组稍高于运动前水平；B组运动后两时相T-LTI均高于A组对应时相，运动后即刻有显著性差异。3.A组运动后即刻CD8~+明显高于运动前，CD4~+、CD4~+/CD8~+明显低于运动前，运动后24h各指标有所恢复，但未达到运动前水平，CD4~+/CD8~+仍明显低于运动前；B组运动后两时相各指标变化趋势与A组相同，但变化幅度均明显小于A组，与运动前比较均无显著性差异，，CD4~+、CD4~+/CD8~+在运动后24h恢复到略高于运动前水平。4.两组受试者运动后即刻IFN-γ均低于运动前，其中A组有显著性差异，B组运动后即刻IFN-γ明显高于A组，运动后24h，两组IFN-γ均回升到接近运动前水平；两组IL-4运动后即刻均非常显著高于运动前，运动后24h有下降，但仍高于运动前水平，其中A组有显著性差异；两组IFN-γ/IL-4运动后即刻非常显著低于运动前，运动后24h有回升，但仍低于运动前，其中A组有显著性差异。5.A组Cor运动后即刻明显高于运动前，运动后24h仍高于运动前，但无显著性差异；B组Cor运动后即刻和运动后24h都低于运动前水平，但无显著性差异。结论：一次大强度跑台运动导致人体T淋巴细胞免疫功能受到抑制,预先补充黄精多糖能减轻运动导致的T淋巴细胞增殖能力的下降、CD4~+细胞含量和IFN-γ水平的降低以及CD8~+细胞含量和IL-4水平的升高，进而预防CD4~+/CD8~+比值、IFN-γ/IL-4比值的下降，表明黄精多糖能有效降低运动导致的机体免疫平衡趋向抑制的程度，减轻免疫稳态向体液免疫偏移的程度。其作用机制可能与黄精多糖能通过抑制运动后血浆皮质醇水平的升高，从而达到减轻其对机体免疫功能的负调节作用有关。
[Abstract]:Aim: to observe the effect of Polygonatum flavescentis polysaccharides on the immune function of human T lymphocytes induced by high intensity exercise, analyze the possible mechanism of its influence, and explore a new method to alleviate the low immune function caused by exercise. Methods: fourteen male students undergoing sports training without systematic major in physical education college were randomly divided into placebo group (group A) and flavin polysaccharide group (group B). The subjects of group A and group B were given placebo and flavin polysaccharides for 4 weeks according to the dose of 1g/d, respectively. A large intensity incremental load treadmill movement, before the exercise, immediately after the exercise, 24 hours after exercise, T lymphocyte transformation index (T-LTI) and T lymphocyte subsets (CD4 ~ + CD8 ~) percentage were measured by MTT assay and flow cytometry respectively. Double antibody sandwich ABC-ELISA assay was used to detect Th1Th 2 type cytokine, and radioimmunoassay was used to detect plasma cortisol. Results 1. There was no significant difference between the two groups before exercise. The T-LTI of the two groups was lower immediately after exercise than that before exercise, and there was a very significant difference between group A and group A. At 24 hours after exercise, the T-LTI of group A was higher than that of group B at 02:00 after exercise, and the T-LTI of group B was higher than that of group A. There was significant difference immediately after exercise. 3. In group A, immediately after exercise, CD8- ~ was significantly higher than that before exercise, CD4- / -CD8- was significantly lower than that before exercise, and the indexes recovered 24 hours after exercise. However, the change trend of each index in group B after exercise was the same as that in group A, but the change range was obviously smaller than that in group A. There was no significant difference between before exercise and before exercise. CD4 ~ / CD4 ~ / CD8 ~ was slightly higher than that of pre-exercise at 24 h after exercise, and the level of CD4 ~ / CD8 ~ was slightly higher than that before exercise. IFN- 纬 in group A was significantly higher than that in group A immediately after exercise, and IFN- 纬 in group B was significantly higher than that in group A immediately after exercise. At 24 hours after exercise, IFN- 纬 in both groups increased to close to the pre-exercise level. Immediately after exercise, IL-4 in both groups was significantly higher than that before exercise, decreased 24 hours after exercise, but still higher than that before exercise, and there was a significant difference in group A, IFN- 纬 / IL-4 immediately after exercise was significantly lower than that before exercise, and there was a rise 24 hours after exercise. The Cor of group A was significantly higher than that of before exercise and 24 hours after exercise. However, there was no significant difference between group B and group B in Cor immediately after exercise and 24 hours after exercise, but the level of Cor in group B was lower than that in group B immediately after exercise and 24 hours after exercise. But there was no significant difference. Conclusion: the immune function of human T lymphocytes was inhibited by a strong treadmill exercise. Supplementation of flavin polysaccharides in advance could attenuate the decrease of proliferation ability of T lymphocytes induced by exercise and the decrease of CD4- 纬 and IFN- 纬 levels, as well as the increase of CD8- cells and IL-4 levels, thereby preventing the decrease of CD4 ~ / CD8- 纬 / IL-4 ratio. The results showed that the polysaccharide could effectively reduce the degree of inhibition of immune balance and the shift of immune homeostasis to humoral immunity. The mechanism may be related to the inhibition of the increase of plasma cortisol after exercise, which can alleviate the negative regulation of the immune function of the body.
【学位授予单位】：成都体育学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：G804.2;R285.5

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