从血压调节系列蛋白基因筛选有氧运动能力分子标记及功能研究

发布时间：2018-05-31 13:22

  本文选题：肾素-血管紧张素-醛固酮系统 + 有氧运动能力　； 参考：《北京体育大学》2014年博士论文

【摘要】：目的：通过分析血压调节系列蛋白基因在运动员和普通人中的分布特征及基因多态性与有氧功能表型指标的关联性,筛选有氧运动能力分子标记；通过阳性标记功能分析,探寻分子调控机制。 方法： 应用MALDI-TOF解析我国优秀耐力运动员和普通对照人群AGT、ACE等12个基因22个SNP位点。应用SPSS、SHEsis及haplo. stats软件分析SNP及单体型在各组分布特征,及其与有氧功能表型指标关联性。构建阳性标记和pcDNA3.1重组质粒,转染293T细胞,检测mRNA表达。 结果： (1) AGTR1基因rs5182位点基因型分布频率在运动员组和对照组间、国际健将组和对照组间、5km运动员组和对照组间以及lOkm运动员组和对照组间均具有显著性差异,但转染细胞培养48小时后,各基因型间mRNA表达量不具有显著性差异。(2)rs4961、rs16843523等位基因及TT单体型分布频率在lOkm运动员组和对照组间具有显著性差异；CYP11B1、CYP11B2基因单体型分布频率在国际健将组和对照组间、马拉松组和对照组间以及女运动员组和女对照组间具有显著性差异。由于样本量较小,上述位点能否作为选材用分子标记有待确认。(3) rs2148582、rs5707、rs11240688、rs4344、rs4343、rs5182、rs1403543,及REN、CYP11B1和CYP11B2(1-3)、ADD1单体型与肺功能指标显著关联。rs2148582、rs11240688、rs4344、rs4363、rs4343、rs6810951、rs11737660、rs7121,及REN、ACE、CYP11B1-CYP11B2单体型与心血管功能指标显著关联。 结论： AGTR1基因rs5182位点可以作为我国北方汉族有氧耐力运动员选材用分子标记,但该位点并非通过AT1R蛋白及mRNA表达量调控运动能力。rs2148582、rs5707、 rs11240688、rs4344、rs4343、rs5182、rs4363、rs6810951、rs11737660、 rs7121、rs1403543,以及REN、ACE、CYP11B1-CYP11B2、ADD1单体型可以作为预测我国北方汉族优秀有氧耐力运动员有氧功能表型指标的候选分子指标。
[Abstract]:Objective: to screen molecular markers of aerobic exercise ability by analyzing the distribution characteristics of blood pressure regulation series protein genes in athletes and ordinary people, and the association between gene polymorphism and aerobic functional surface index, and to analyze the function of positive markers. To explore the mechanism of molecular regulation. Methods: MALDI-TOF was used to analyze 22 SNP loci of 12 genes such as AGTN ACE in Chinese elite endurance athletes and general control population. The distribution characteristics of SNP and haplotype in each group were analyzed by SPSS SHEsis and haplo. stats software. The positive labeled and pcDNA3.1 recombinant plasmids were constructed and transfected into 293T cells to detect the expression of mRNA. Results: 1) there were significant differences in the distribution frequency of AGTR1 gene rs5182 genotype between athletes and control groups, between international athletes and control groups, and between lOkm athletes and control groups. But after 48 hours of culture, There was no significant difference in mRNA expression among different genotypes. The allele frequency of rs4961rs16843523 and the frequency of TT haplotype distribution were significantly different between the lOkm athletes and the control group. The distribution frequency of CYP11B1CY CYP11B2 haplotype was found between the international athletes and the control group. There were significant differences between the marathon group and the control group and between the female athletes group and the female control group. 鐢变簬鏍锋湰閲忚緝灏，

本文编号：1959853