有氧运动对大鼠脑衰老和学习记忆能力及小脑BDNF表达的影响

发布时间：2018-06-02 15:50

本文选题：有氧运动 + 脑衰老　；参考：《成都体育学院》2017年硕士论文

【摘要】：目的:SD雄性大鼠腹腔注射D-半乳糖制作衰老模型,在大鼠造模前、造模过程中、造模后进行有氧运动(游泳,60min/d)干预,检测大鼠脑衰老指标、空间学习记忆能力、小脑脑源性神经营养因子BDNF的表达,探讨有氧运动对大鼠脑衰老、学习记忆能力及小脑BDNF的影响。方法:将SD大鼠(72只,3月龄,雄性)按照SD(造模前有氧运动预处理组)、ND(造模组)、NNa(注射生理盐水对照组)、NDs(造模过程中有氧运动组)、NDS(造模后有氧运动组)及NDN(造模后自然喂养组)随机分为6个大组。在造模结束后每个大组随机分为2小组:M组(SDM组、NDM组、NNa M组、NDs M组、NDSM组、NDNM组),M组进行7天的Morris行为学实验;N组(SDN组、NDN组、NNa N组、NDs N组、NDSN组、NDNN组),N组进行7天的自然喂养;总共12组(n=6)。分组后对SD大鼠进行衰老造模(D-半乳糖,100mg/kg/d);在大鼠造模不同时期进行有氧运动,使用Morris水迷宫行为学测试检测空间学习记忆能力;结束后检测各组大鼠大脑中自由基相关指标(超氧化物歧化酶SOD、谷胱甘肽过氧化酶GSH-PX、丙二醛MDA)的含量;使用real-time PCR、Western-blot等实验技术检测各组大鼠小脑中BDNF的表达情况。结果:(1)SD大鼠NNa组大鼠状态很好、行动快捷、生活饮食正常、毛发白亮;ND组的大鼠出现了明显的自然衰老特征,毛发枯黄、行动迟缓、食欲欠佳、嗜睡。(2)自由基检测结果:SDN组MDA的含量和SOD、GSH-Px的活性较NDN组差异无显著性(P0.05);NDs N组MDA的含量显著低于NDN组(P0.05),SOD(P0.05)、GSH-Px(P0.05)的活性显著高于NDN组;NDSN组MDA含量显著低于NDNN组(P0.05),SOD、GSH-Px活性显著性高于NDNN组(P0.05)。(3)水迷宫定位航行实验结果:各组大鼠的平均逃避潜伏期随着训练次数的增加逐渐减少且趋于稳定,不同组别之间的平均逃避潜伏期具有一定的差异;各组在第2天的平均逃避潜伏期的时间比第1天少(P0.05);各组(除NDSM组)在第3天的平均逃避潜伏期的时间较第2天少(P0.05);NNa M组、NDs M组的平均逃避潜伏期的时间从第3天开始趋于稳定(P0.05);但SDM组、NDM组、NDSM组、NDNM组的平均逃避潜伏期的时间在第4天才慢慢趋于稳定(P0.05),经过第1天的定位航行实验,NNa M组的平均潜逃避伏期的时间较NDM组少(P0.05),NDSM组和NDNM组平均潜逃避伏期的时间慢慢增高(P0.05),在第6天的定位航行实验中,SDM组和NDM组平均潜逃避伏期的时间差异无显著性(P0.05),与NDNM组相比,NDSM组的大鼠平均潜逃避伏期的时间显著性降低(P0.05)。空间(第2象限入水)探索实验表明:SDM组穿越原站台次数与NDM组差异无显著性(P0.05);NDs M组穿越原站台次数显著高于NDM组(P0.05);NNa M组穿越原站台次数显著高于NDM组(P0.05);NDSM组穿越原站台次数显著高于NDNM组(P0.05)。(4)小脑BDNF real-time PCR和Western-blot检测结果:NNa N组小脑BDNF m RNA(P0.05)和蛋白的表达量(P0.05)显著高于NDN组;SDN组小脑BDNF m RNA较NDN组差异无显著性(P0.05),SDN组小脑BDNF蛋白质的表达量较NDN组差异无显著性(P0.05);NDs N组小脑BDNF m RNA(P0.05)和蛋白的表达量(P0.05)显著性高于NDN组,NDSN组小脑BDNF m RNA表达显著性高于NDNN组(P0.05),蛋白质量的表达显著性高于NDNN组(P0.05),M组与其相对应N组变化趋势相同,组间无显著差异(P0.05)。结论:(1)造模后SD大鼠的形态学特征和各自由基相关指标证明衰老模型造模成功。(2)造模前对SD大鼠进行有氧运动干预,大鼠小脑BDNF的表达没有上调,大鼠空间学习记忆能力没有明显的变化。(3)在造模过程中和造模后对SD大鼠进行有氧运动干预,能上调大鼠小脑BDNF的表达,提高大鼠空间学习记忆能力。(4)实验证明有氧运动上调大鼠小脑BDNF的表达,可能是延缓大鼠空间学习记忆能力衰退的机制之一。
[Abstract]:Objective: SD male rats were intraperitoneally injected with D- galactose to make aging model. In the process of model building, aerobic exercise (swimming, 60min/d) was used to intervene in the rat model before model building, and the index of brain senescence, spatial learning and memory, and the expression of cerebellar neurotrophic factor BDNF were detected in rats, and the brain senescence and learning and memory of aerobic exercise on rat brain were discussed. Methods: the effects of ability and cerebellar BDNF. Methods: the SD rats (72, 3 month old, male) were randomly divided into 6 large groups by SD (pre model aerobic exercise preconditioning group), ND (model group), NNa (injection physiological saline control group), NDs (aerobic exercise group during molding process), NDS (after model oxygenation group) and NDN (after model natural feeding group). Each group was randomly divided into 2 groups: group M (group SDM, group NDM, NNa M group, NDs M group, NDSM group, NDNM group), M group for Morris behavioral experiment for 7 days; N groups were fed with 7 days of natural feeding. Aerobic exercise was carried out at different stages and the Morris water maze behavior test was used to test the spatial learning and memory ability. After the end, the content of free radical related indexes (superoxide dismutase SOD, glutathione peroxidase GSH-PX, malondialdehyde MDA) in the brain of each group was detected, and real-time PCR, Western-blot and other experimental techniques were used to detect each group. The expression of BDNF in the cerebellum of rats. Results: (1) the rats in group NNa of SD were in good state, quick action, normal diet and white hair; the rats in group ND had obvious natural aging characteristics, hair yellow, slow action, poor appetite, lethargy. (2) the content of MDA in group SDN and SOD, GSH-Px activity was more than NDN group. The content of MDA in group NDs N was significantly lower than that of NDN group (P0.05), SOD (P0.05), GSH-Px (P0.05) activity was significantly higher than that of NDN group, and the activity of NDSN group was significantly lower than that of the NDN group. (3) the results of water maze navigation experiment: the average escape latency of rats in each group was trained with the training. The average evasion latency between different groups had a certain difference, the average escape latency of each group was less than that of first days (P0.05), and the average escape latency of each group (NDSM group) at third days was less than that of second days (P0.05), and the average escape latency in group NNa M and NDs M group. The period of time began to stabilize from third days (P0.05), but the average escape latency of group SDM, NDM, NDSM and NDNM gradually stabilized at fourth days (P0.05). After first days of navigation experiment, the average escape time of NNa M group was less than that of the NDM group (P0.05), while the average escape time of NDSM and NDNM groups was slow. Man Zenggao (P0.05), in the sixth day positioning navigation experiment, the time difference between the SDM group and the NDM group had no significant difference (P0.05). Compared with the NDNM group, the average escape time of the rats in the NDSM group was significantly lower (P0.05). The space (second quadrant water entry) exploration experiment showed that the number of SDM groups crossing the original platform was not different from the NDM group. The number of NDs M traversing the original platform was significantly higher than that of the NDM group (P0.05), and the number of NNa M groups crossing the original platform was significantly higher than that of the NDM group (P0.05), and the number of NDSM groups across the original platform was significantly higher than that of the NDNM group (P0.05). (4) the expression of cerebellar cerebellum and protein was significantly higher than that of the NDNM group (4). There was no significant difference between the cerebellar BDNF m RNA in the SDN group and the NDN group (P0.05), and there was no significant difference in the expression of BDNF protein in the cerebellum of SDN group compared with that of the NDN group (P0.05). The significant sex of the cerebellar cerebellar and protein expression was significantly higher than that of the NDN group (P0.05). The significance of the expression was higher than that of the NDNN group (P0.05), and there was no significant difference between the M group and the corresponding N group (P0.05). Conclusion: (1) the morphological characteristics of the SD rats and the related indexes of the free radicals proved that the model of the aging model was successful. (2) the aerobic exercise intervention was carried out on SD rats before the modeling, and the expression of BDNF in the cerebellum of the rat was not up. There was no obvious change in the ability of spatial learning and memory in rats. (3) aerobic exercise intervention on SD rats in the process of modeling and after modeling could increase the expression of BDNF in the cerebellum of rats and improve the spatial learning and memory ability of rats. (4) the experiment showed that aerobic exercise increased the expression of BDNF in the rat cerebellum, which may delay the spatial learning and memory ability of rats. One of the mechanisms of the recession.
【学位授予单位】：成都体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R87

【参考文献】