强制性运动疗法联合白脉散有效成分组治疗脑卒中运动功能障碍的研究

发布时间：2018-06-02 19:28

  本文选题：强制性运动疗法 + 白脉散有效成分组　； 参考：《苏州大学》2012年硕士论文

【摘要】：研究目的：研究强制性运动疗法（CIMT）和白脉散有效成分组（ECG）对脑损伤运动功能障碍的影响，阐明可能的作用机制，为相应的临床研究提供理论和实验依据。 研究方法：本实验以二月龄雄性SD大鼠为实验对象，线栓法制备大脑中动脉栓塞（MCAO/R）致中枢神经性运动功能障碍模型，合格大鼠分为6组(每组10只)：模型组（MCAO/R，静注溶媒）、阳性对照组（MCAO/R，静注银杏叶提取物EGB）、强制性运动疗法组（MCAO/R，运动疗法，CIMT组）、ECG组（MCAO/R，静注ECG）、联合治疗组（MCAO/R，运动和静注ECG组）、假手术组(Sham，静注溶媒)。造模后分组干预：CIMT组（15min/次，1次/d×7d），ECG组（150mg.kg-1.d-1，1次/d×7d），EGB组（5.6mg.kg-1.d-1，1次/d×7d）。第7d干预结束后即刻全部处死。测试下列指标：脑缺血灶组织病理形态学结构观察（HE染色）、细胞凋亡数量（TUNEL染色）、试剂盒检测自由基相关指标（SOD活性、MDA含量、GSH含量、NOS活性）、免疫组化观察脑缺血灶皮层凋亡相关蛋白（Caspase-3及Bcl-2蛋白）表达，体重（测定时间：干预前、干预后3d、7d）、神经行为学评分（Long5级4分法；评估时间：干预前、实验结束即刻）。实验结果采用SPSS l8.0统计软件进行分析。 研究结果：7d干预结束后四个干预组各指标较MCAO/R组均有显著改善：1、四个干预组的体重均有所恢复。2、四个干预组的神经行为学评分、脑组织病理形态学损伤、缺血灶皮层细胞凋亡数量均显著改善，改善程度从高到低依次为：CIMT+ECG组 CIMT组 ECG组≈EGB组。3、四个干预组的脑组织中SOD活性、GSH含量、SOD/MDA比值均显著增高，而MDA含量、NO含量显著降低，各干预组抗自由基能力从高到低依次为：CIMT+ECG组 EGB组CIMT组≈ECG组。4、四个干预组的脑缺血皮层区细胞中Bcl-2蛋白表达均显著增高，从高到低依次为：CIMT+ECG组 CIMT组 ECG组≈EGB组；Caspase-3蛋白表达均显著减少，从低到高依次为：CIMT+ECG组CIMT组ECG组≈EGB组。 研究结论：1、本研究制备的脑缺血再灌注模型是有效的。2、CIMT和/或静注ECG、EGB可以促进脑损伤运动障碍的康复，发现以上疗法可以提高大脑皮层的抗氧化物质含量和抗氧化能力，减少脑皮层梗死体积，减少凋亡相关蛋白含量，减少细胞凋亡和丢失，促进了运动功能的恢复。3、CIMT与BMECG对脑损伤运动功能障碍大鼠的保护效果具有相同作用，前者效果好于后者，且均好于EGB（金标准），两者联用效果更佳，，说明实验采取的干预措施是有效的。
[Abstract]:Objective: to study the effect of compulsory exercise therapy (CIMT) and Baimaisan active component group (ECG) on motor dysfunction of brain injury, to clarify the possible mechanism of action and to provide theoretical and experimental basis for clinical study. Methods: in this study, the male SD rats of two months of age were used to establish the model of central nervous system motor dysfunction induced by middle cerebral artery embolization (MCAO / R). The eligible rats were divided into 6 groups (10 rats in each group): model group (MCAO / R, intravenous injection of solute), positive control group (MCAO / R), intravenous injection of Ginkgo biloba extract (EGBN), mandatory exercise therapy group (MCAO / R), exercise therapy / CIMT group / ECG group, intravenous injection of ECG / R, combined treatment group (MCAO / R), exercise and intravenous injection (MCAO / R). ECG group, sham group, intravenous injection of solute. After the model was established, the rats were divided into two groups: the control group (15 min / d 脳 7 d) and the ECG group (n = 150 mg 路kg ~ (-1) 路d ~ (-1) 路d ~ (-1) / d ~ (-1) 路L ~ (-1) 路d ~ (-1) 路d ~ (-1)? All rats were killed immediately after 7 days of intervention. The following indexes were tested: histopathologic structure of cerebral ischemia focus was observed by HE staining, apoptosis was detected by Tunel staining, SOD activity, MDA content, GSH content and NOS activity were detected by kit, and cerebral ischemia was observed by immunohistochemistry. Expression of Caspase-3 and Bcl-2 protein in focal cortex. Body weight (measurement time: before intervention, 3 days after intervention, 7 days after intervention, neurobehavioral score and Long5 grade 4; evaluation time: before intervention, immediately after intervention. The experimental results were analyzed by SPSS l 8.0 software. Results after 7 days of intervention, each index of the four intervention groups was significantly improved than that of the MCAO/R group. The body weight of the four intervention groups recovered to 0.2. The neurobehavioral score and the pathomorphological injury of brain tissue in the four intervention groups were observed. The number of apoptotic cells in ischemic cortex was significantly improved, the degree of improvement from high to low was as follows: CIMT group, CIMT group, 鈮

本文编号：1969918