病证结合大鼠辐射损伤模型构建初探

发布时间：2018-06-08 07:27

本文选题：病证结合 + 动物模型　；参考：《中华中医药杂志》2017年05期

【摘要】：目的:建立一种稳定的、可操作性强的病证结合大鼠辐射损伤模型,以满足研究药物对辐射损伤防治评价的需求。方法:采用碳离子(~(12)C~(6+))束照射大鼠右侧肺部构建辐射损伤动物模型,同时设立正常对照组、单纯辐射组、辐射+黄芪汤组,从病因、症状、客观指标、药物反证等方面对辐射损伤大鼠模型进行综合判断,验证该模型的中医证候属性。结果:单纯辐射组大鼠的症状体征(摄食量、饮水量、心率、肛温、排尿量、体质量)、血象(WBC与RBC)、脾脏、胸腺指数等指标与正常对照组比较,差异均有统计学意义(P0.01)。辐射+黄芪汤组可明显改善大鼠的主观症状和上述客观指标,差异有统计学意义(P0.01)。结论:~(12)C~(6+)束照射大鼠辐射损伤模型的中医证候属性是气阴两虚证。
[Abstract]:Objective: to establish a stable and operable model of radiation injury in combination of disease and syndrome in order to meet the needs of drug research on radiation damage prevention and treatment. Methods: the animal model of radiation injury was established by irradiation of the right lung of the rat with the carbon ion (C12) and the radiation group was established as control group, simple radiation group and radiative Huangqi decoction group, according to the etiology, symptoms and objective indexes of the model, the animal model of radiation injury was established in the right lung of the rats. The model of radiation injury was judged synthetically, and the TCM syndrome attribute of the model was verified. Results: the symptoms and signs (intake, drinking water, heart rate, anus temperature, urination volume, body weight, WBC and RBCU, spleen and thymus index) of the rats in the radiation group were significantly different from those in the normal control group (P 0.01). Radiation Huangqi decoction group could obviously improve the subjective symptoms and the above objective indexes of rats, the difference was statistically significant (P 0.01). Conclusion the TCM syndromes of radiation injury model of rats irradiated with the beam are qi and yin deficiency syndrome.
【作者单位】：甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室;甘肃中医药大学博士后流动站;甘肃中医药大学附属医院;
【基金】：国家自然科学基金地区项目(No.81460695) 中国博士后科学基金第60批面上项目(No.2016 M602887)~~
【分类号】：R-332;R818

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