随机回转模拟失重对成骨细胞MACF1表达和β-catenin信号通路的影响

发布时间：2018-06-16 13:38

本文选题：模拟失重 + 微管微丝交联因子　；参考：《航天医学与医学工程》2017年01期

【摘要】：目的研究随机回转(RPM)模拟失重条件对微管微丝交联因子1(MACF1)表达及β-catenin信号通路的影响,为失重性骨丢失机制研究提供实验依据。方法 RPM条件培养成骨细胞,PCR检测MACF1mRNA表达变化,Western blot检测MACF1以及不同磷酸化位点β-catenin蛋白表达变化,荧光素酶报告基因检测细胞β-catenin荧光素酶活性变化。结果 RPM模拟失重条件下,成骨细胞MACF1表达下降,β-catenin mRNA和不同位点磷酸化蛋白表达量发生改变;MACF1 shRNA抑制了成骨细胞中β-catenin表达,影响了β-catenin蛋白稳定性及活性。结论 RPM模拟失重抑制了MACF1表达和β-catenin表达及活性;MACF1 shRNA影响了β-catenin表达及活性。MACF1可能是一种力敏感分子,通过调控β-catenin参与成骨细胞对力学信号的感知和响应。
[Abstract]:Objective to study the effects of simulated weightlessness on the expression of microtubule microfilament crosslinking factor 1 (MACF1) and 尾 -catenin signaling pathway, and to provide experimental evidence for studying the mechanism of weightlessness bone loss. Methods the expression of MACF1 mRNA in cultured osteoblasts was detected by polymerase chain reaction (RPM). The expression of MACF1 and 尾 -catenin at different phosphorylation sites was detected by Western blot. The luciferase reporter gene was used to detect the activity of 尾 -catenin luciferase. Results under RPM simulated weightlessness, the expression of MACF1 in osteoblasts decreased, and the expression of 尾 -catenin mRNA and phosphorylated protein at different sites changed. MACF1 shRNA inhibited the expression of 尾 -catenin in osteoblasts and affected the stability and activity of 尾 -catenin protein. Conclusion simulated weightlessness of RPM inhibits the expression of MACF1 and the expression and activity of 尾 -catenin. MACF1 shRNA may affect the expression and activity of 尾 -catenin. MACF1 may be a force sensitive molecule. 尾 -catenin is involved in the sensing and response of osteoblasts to mechanical signals by regulating 尾 -catenin.
【作者单位】：西北工业大学生命学院空间生物实验模拟技术国防重点学科实验室骨代谢研究室;
【基金】：国家自然科学基金资助项目(31570940)
【分类号】：R852.22

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