Beagle犬良性前列腺增生模型的制作及介入栓塞方法探讨

发布时间：2018-06-18 01:24

本文选题：良性前列腺增生 + 动物模型　；参考：《影像诊断与介入放射学》2016年04期

【摘要】：目的介绍运用丙酸睾酮(testosterone propionate,TP)药物诱导制作Beagle犬良性前列腺增生动物模型,并探讨介入栓塞方法的运用。方法实验对象为雄性Beagle犬16只,随机分为2组:模型组(n=8)、对照组(n=8)。其中模型组去势1个月后给予TP 2.5 mg/kg/d肌肉注射,连续3个月。16只Beagle犬均行MRI检查观察前列腺体积,分别于去势后1个及4个月行MRI扫描。4个月后模型组行股动脉插管造影观察双侧前列腺供血动脉,并行前列腺动脉栓塞,根据选用导管类型分为2组:罗伯特导管(Roberts uterine catheter,RUC)组(n=4)、眼镜蛇导管(Cobra)组(n=4)。结果模型组Beagle犬去势后运用TP药物诱导3个月后均成功建立良性前列腺增生模型,其与去势前相比体积均显著增大(P0.05),对照组前列腺体积无显著变化(P0.05)。模型组前列腺动脉均明确显示,血管增粗。RUC组插管成功率、超选择栓塞率高于Cobra组,手术时间短于Cobra组。结论运用TP药物诱导Beagle犬良性前列腺增生是前列腺增生介入治疗实验研究较为理想的动物模型。MRI能准确评估前列腺的体积变化。股动脉插管后用RUC导管血管造影观察双侧前列腺动脉成功率高,可完成超选择栓塞,值得推广。
[Abstract]:Objective to introduce the use of testosterone propionate (TP) to induce the animal model of benign prostatic hyperplasia in Beagle dogs, and to explore the application of interventional embolization. Methods 16 male Beagle dogs were randomly divided into 2 groups: model group (n=8) and control group (n=8). The model group was given TP 2.5 mg/kg/d muscle after 1 months. The volume of prostate was observed by MRI examination for 3 months after 3 months of meat injection. After 1 and 4 months after the castration, the MRI scan was performed for.4 months after the castration. The femoral artery catheterization was performed in the model group to observe the bilateral prostatic blood supply arteries and the prostate artery embolization. According to the selection of the catheter type, the 2 groups were divided into the Robert catheter (Roberts uterine cathete). The R, RUC) group (n=4) and the cobra catheter (Cobra) group (n=4). Results the benign prostatic hyperplasia model was successfully established after 3 months after the castration of Beagle canine in the model group. The volume of the benign prostatic hyperplasia was significantly increased (P0.05) and the prostate volume in the control group was no significant change (P0.05). The prostate arteries of the model group were all clearly demonstrated and increased. The success rate of tracheal intubation in.RUC group was higher than that in group Cobra, and the operation time was shorter than that of group Cobra. Conclusion using TP drugs to induce Beagle dog benign prostatic hyperplasia is an ideal animal model of interventional therapy for prostatic hyperplasia,.MRI can accurately assess the volume transformation of the prostate. After femoral artery intubation, RUC catheter angiography is used. The success rate of bilateral prostatic artery is high, and superselective embolization can be achieved.
【作者单位】：华中科技大学同济医学院附属同济医院放射科;
【基金】：国家自然科学基金(81171307)
【分类号】：R697.3;R815

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