运动对慢性帕金森病模型小鼠脑部谷胱甘肽及神经营养因子的调控作用

发布时间：2018-06-21 11:34

本文选题：帕金森症 + 行为学测试　；参考：《扬州大学》2014年硕士论文

【摘要】：目的：在成功建立小鼠慢性帕金森模型的基础上,研究运动对帕金森症小鼠在行为学上的促进作用,探究运动对小鼠脑部谷胱甘肽、以及神经营养因子的干预作用。方法：选用清洁级雄性C57BL/6J小鼠,8周龄40只,体重24+3g,常规条件喂养,随机将小鼠分为5组,分别为：健康安静组(A组,n=8)、MPTP注射组(B组,n=8)、健康运动组(C组,n=8)、运动+MPTP注射组(D组,n=8)、MPTP注射+运动组(E组,n=8),对实验组小鼠注射MPTP,建立小鼠慢性帕金森症模型。各组运动组小鼠每天进行中等强度转轮训练(20转/份),每次30分钟,连续4周。对各阶段小鼠进行行为学测试及体重测定,取材前12小时禁食水,并对小鼠进行体重、行为学检测。最后一次运动刺激后进行取材,检测各组小鼠脑部GSH含量、GSH-PX活性、BDNF、GDNF蛋白表达水平等指标。结果： 1.通过对小鼠腹腔注射MPTP (溶于9g/L生理盐水中,终浓度为1g、L),每周2次,间隔3天,每次15mg/kg,连续4周成功建立慢性帕金森症小鼠模型。 2.运动组行为学测试成绩与安静组相比差异显著(P0.01),D、E两组与B组相比较有显著性差异(P0.01)。而D组与E组相比,有运动基础的D组小鼠行为学测试的数据稍好于E组小鼠(P0.05)。 3.MPTP组动物脑内GSH含量明显减少、GSH-PX活性明显降低,安静健康组小鼠与安静运动组小鼠在GSH含量及GSH-PX活性上都存在显著性差异(P0.01),而D组小鼠与E组小鼠在两者比较中都显示出一定差异(P0.05),并体现出一定趋势。 4.B组与D、E组小鼠蛋白表达存在差异(P0.01),显示运动可以增加帕金森小鼠脑内BDNF、GDNF的蛋白表达,同时D组小鼠与E组小鼠脑部BDNF、GDNF蛋白相对含量也存在差异(P0.05),显示有长期运动基础及运动习惯的小鼠,脑内BDNF、GDNF蛋白含量高于康复运动组小鼠。结论： 1.有氧运动可改善帕金森症小鼠行为学表现及其肢体运动功能。 2.有氧运动的练习,对PD小鼠脑内GSH含量的提高,GSH-PX活性的增强有显著的作用,使对神经细胞有害的羟自由基受到抑制,神经细胞得到保护或恢复。 3.通过运动可提高PD小鼠脑内对神经元具有显著保护和促进修复作用的BDNF、GDNF蛋白表达水平,揭示运动对多巴胺能神经元的保护和修复作用。
[Abstract]:Aim: on the basis of the successful establishment of chronic Parkinson's model in mice, the effects of exercise on the behavior of mice with Parkinson's disease were studied, and the effects of exercise on glutathione and neurotrophic factor in brain of mice were investigated. Methods: 40 C57BL / 6J C57BL / 6J C57BL / 6J C57BL / 6J mice were randomly divided into 5 groups. The rats in the healthy and quiet group were injected with MPTP in group A, group B in group B, group C in healthy exercise group, group D in group D and group E, respectively. The model of chronic Parkinson's disease was established by injection of MPTPin the experimental group. The mice in the exercise group were treated with moderate intensity wheel training for 20 cycles per day for 30 minutes for 4 weeks. Behavioral test and body weight measurement were carried out in each stage of mice, and fasting water was taken 12 hours before sampling, and the body weight and behavior of mice were measured. The GSH content and GSH-PX activity of the brain and the expression level of BDNF GDNF protein were measured after the last exercise stimulation. Results: 1. A mouse model of chronic Parkinson's disease was successfully established by intraperitoneal injection of MPTP (dissolved in 9 g / L normal saline with a final concentration of 1 g / L) twice a week for 3 days at a interval of 15 mg / kg for 4 weeks. The scores of behavioral test in exercise group were significantly different from those in quiet group (P 0.01). There was significant difference between two groups (P 0.01) and group B (P 0.01). Compared with group E, the behavioral test data of group D with exercise basis were slightly better than that of group E (P 0.05). 3. The activity of GSH-PX in the brain of rats in MPTP group was significantly reduced, and the activity of GSH-PX was significantly decreased in group D than that in group E, and the activity of GSH-PX in group D was significantly lower than that in group E. There was a significant difference in GSH content and GSH-PX activity between the healthy group and the rest exercise group, while there was a certain difference between group D and E mice in the content of GSH and the activity of GSH-PX, and showed a certain trend. 4. There was a difference in protein expression between group B and group DNE, indicating that exercise could increase the expression of BDNF GDNF in the brain of Parkinson's mice. At the same time, the relative content of BDNF GDNF protein in the brain of group D and E mice was also different (P 0.05), which showed that the content of BDNFN GDNF protein in brain of group D mice was higher than that of mice with rehabilitation exercise group. Conclusion: 1. Aerobic exercise can improve the behavior of Parkinson's disease mice and limb motor function. 2. The exercise of aerobic exercise had a significant effect on the increase of GSH content in brain of PD mice and the increase of GSH-PX activity, which inhibited the harmful hydroxyl radicals and protected or restored the nerve cells. Exercise can improve the expression level of BDNF GDNF protein which can significantly protect and promote the repair of neurons in the brain of PD mice, and reveal the protective and repair effects of exercise on dopaminergic neurons.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：G804.2;R87

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