不同强度运动对肥胖大鼠肝脏氧化应激和脂联素及其受体的影响

发布时间：2018-06-22 21:17

本文选题：不同强度运动 + 氧化应激　；参考：《中国运动医学杂志》2017年09期

【摘要】：目的:探讨三种不同强度运动对肥胖大鼠肝脏氧化应激及脂联素/脂联素受体等相关指标的影响。方法:经16周高脂饲料喂养构建肥胖大鼠模型,32只模型鼠分为高脂安静组(HF,8只)、小强度运动组(HS组,8只)、中等强度运动组(HM组,8只)和递增负荷运动组(HI组,8只),另外选取正常对照组(NC组)大鼠8只。运动方式采用跑台运动,小强度运动跑速为10 m/min,中等强度跑速为15 m/min,递增负荷强度为10 m/min的速度运动20 min、20 m/min的速度运动20 min、28~30 m/min的速度运动20min。运动组每周运动5天,每次1小时。干预持续6周后检测各组大鼠体重,肝脏超氧化物歧化酶(SOD)活性、丙二醛(MDA)、肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、脂联素受体I(AR1)、AR2,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、脂联素、甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、自由脂肪酸(FFA)等指标。结果:与NC组比较,HF组大鼠体重、TG、TC、LDL-C、FFA含量、ALT活性、AST活性以及MDA、TNF-α和IL-6表达均显著性升高(P0.05或P0.01),HDL-C含量、SOD活性、血清脂联素水平及AR1、AR2表达均显著性降低(P0.05或P0.01);与HF组比较,HS、HM和HI组体重、血清TG、LDL-C、FFA含量及肝脏ALT含量、MDA、TNF-α、IL-6表达均显著性降低(P0.05或P0.01),SOD活性、脂联素水平及AR1、AR2表达均显著性升高(P0.05或P0.01),仅HM组HDL-C含量显著升高(P0.05);与HS组和HI组比较,HM组FFA含量、MDA表达进一步降低(P0.05),脂联素水平、AR2进一步增加(P0.05)。结论:有氧运动能有效改善高脂饮食所致的肥胖大鼠脂代谢异常,提高肝脏抗氧化酶活性,增强自由基清除能力,预防肝细胞炎症损伤,改善肝脏代谢功能紊乱,其中中等强度运动优于小强度或递增负荷强度运动,其机制可能是通过增加血清脂联素进一步激活肝脏脂联素受体2有关。
[Abstract]:Aim: to investigate the effects of three different intensities of exercise on liver oxidative stress and adiponectin / adiponectin receptor in obese rats. Methods: after 16 weeks of high fat diet feeding, 32 obese rats were divided into four groups: hyperlipidemia group (8 rats), low intensity exercise group (HS group, 8 rats), moderate intensity exercise group (HM group, 8 rats) and increasing load exercise group (HI group, 8 rats). In addition, 8 normal control group (NC group) rats were selected. The treadmill movement was used, the low intensity running speed was 10 m / min, the moderate intensity running speed was 15 m / min, the incremental load intensity was 10 m/min, the speed movement was 20 mins and 20 m/min, 20 mins and 2830 m/min. Exercise group 5 days a week, 1 hour each time. Body weight, liver superoxide dismutase (SOD), malondialdehyde (MDA), tumor necrosis factor- 伪 (TNF- 伪), interleukin-6 (IL-6), adiponectin receptor I (AR1) AR2, serum alanine aminotransferase (alt), aspartate aminotransferase (AST), adiponectin were measured after 6 weeks of intervention. Triglyceride (TG), cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), free fatty acid (FFA) and so on. Results: compared with NC group, the body weight of rats in HF group was significantly increased (P0.05 or P0.01), serum adiponectin level and AR1 / AR2 expression were significantly decreased (P0.05 or P0.01), and compared with HF group, the activity of alt and the expression of MDA-TNF- 伪 and IL-6 were significantly increased (P0.05 or P0.01), and the body weight of HSHM and HI group was significantly lower than that of HF group (P0.05 or P0.01), and compared with that of HF group, the activity of alt and the expression of MDA-TNF- 伪 and IL-6 were significantly increased (P0.05 or P0.01). The levels of serum TGG LDL-CfFA and liver alt were significantly decreased (P0.05 or P0.01), and the activity of SOD was significantly decreased in MDA-TNF- 伪 and IL-6 (P0.05 or P0.01), and the expression of TNF- 伪 and IL-6 decreased significantly (P0.05 or P0.01). Adiponectin level and AR1P AR2 expression were significantly increased (P0.05 or P0.01), only HDL-C content in HM group was significantly increased (P0.05), compared with HS group and HI group, FFA content and MDA expression in HM group were further decreased (P0.05), adiponectin level and AR2 level were further increased (P0.05). Conclusion: aerobic exercise can effectively improve lipid metabolism of obese rats induced by high fat diet, increase liver antioxidant enzyme activity, enhance free radical scavenging ability, prevent hepatocyte inflammation injury and improve liver metabolic disorder. The moderate intensity exercise is superior to small intensity exercise or increasing load intensity exercise, and the mechanism may be related to the further activation of hepatic adiponectin receptor 2 by increasing serum adiponectin.
【作者单位】：广东第二师范学院体育学院;华南师范大学体育科学学院;广东医学院;
【基金】：广东省科学事业计划项目(2011B031600010) 广东省体育局科研项目(GDSS2016115)
【分类号】：R87

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