硫化氢对创伤应激引起大鼠急性肝损伤的作用

发布时间：2018-06-25 13:18

本文选题：法医病理学 + 创伤和损伤　；参考：《法医学杂志》2016年02期

【摘要】：目的探讨硫化氢(H_2S)在挤压大鼠后肢致急性肝损伤过程中的作用。方法将大鼠随机分为对照组、挤压组、H_2S供体硫氢化钠(Na HS)+挤压组、H_2S生成抑制剂炔丙基甘氨酸(propargylglycine,PAG)+挤压组。用标准重物挤压大鼠后肢建立急性肝损伤模型,分别于挤压结束后30、120 min处死大鼠。比色法检测血清天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活性,化学法检测大鼠血浆H_2S、肝组织丙二醛(malondialdehyde,MDA)、蛋白质羰基、谷胱甘肽(glutathion,GSH)含量和H_2S生成酶胱硫醚γ-裂解酶(cystathionineγ-lyase,CSE)活性,RT-PCR半定量检测肝组织CSE m RNA的表达。结果挤压大鼠后肢可引起血清AST、ALT活性增高,肝组织MDA、蛋白质羰基含量增加及GSH含量降低,血浆H_2S含量减少,肝组织CSE活性降低、CSE m RNA表达下降。预先给予Na HS可显著减轻、而PAG明显加剧挤压后肢所致上述肝损伤改变。结论 H_2S生成减少参与介导创伤应激引起的大鼠急性肝损伤。
[Abstract]:Objective to investigate the role of hydrogen sulfide (H _ 2S) in the process of acute liver injury induced by extrusion of hind limbs in rats. Methods the rats were randomly divided into two groups: the extruded group with H2S donor sodium sulfohydride (NaHS) extruding group and the extruded group with propargyl glycine PAG (propargyl glycine) as an inhibitor of H2S production. The model of acute liver injury was established by pressing the hind limbs of rats with standard weight. The rats were killed at 30120 min after extrusion. The activities of aspartate aminotransferase (AST) and alanine aminotransferase (alt) were detected by colorimetric method. The plasma H2S, malondialdehyde (MDA) and protein carbonyl group were detected by chemical method in rat plasma. The content of glutathione (GSH) and the activity of cystathionine 纬 -lyase (cystathionine 纬 -lyase) were used to detect the expression of CSE mRNA in liver tissue. Results the results showed that the activity of ASTO alt in serum was increased, the content of protein carbonyl and GSH in liver tissue was increased, the content of H2S in plasma was decreased, the activity of CSE in liver was decreased and the expression of CSE mRNA in liver was decreased. Pretreatment with NaHS significantly alleviated the liver injury caused by compression of the hind limbs, while PAG significantly aggravated the changes. Conclusion the reduction of H2S production is involved in acute liver injury induced by traumatic stress in rats.
【作者单位】：南通大学医学院法医学系;苏州大学医学部;
【基金】：国家自然科学基金资助项目(81273341) 江苏省高校优势学科建设工程项目(PAPD)
【分类号】：D919

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