D11S4463和D17S974基因座在汉族人群中遗传多态性及其等位基因分型标准物的制备
本文选题：法医物证学 + 等位基因分型标准物 ； 参考：《山西医科大学》2013年硕士论文
【摘要】：目的 调查D11S4463基因座和D17S974基因座在中国汉族人群中的遗传多态性,并用分子克隆技术制备其等位基因分型标准物。 方法 用荧光PCR和ABI3130XL遗传分析仪对中国汉族600份无关个体血斑样本进行D11S4463和D17S974基因座遗传多态性调查,并用分子克隆的方法构建其等位基因分型标准物。首先在人群中分别筛选出D11S4463基因座的9个等位基因和D17S974基因座的8个等位基因,经PCR扩增,并将纯化后的扩增产物与pMD18-T质粒载体连接后转化到DH5α感受态大肠杆菌中；筛选重组子及提取重组质粒,对等位基因重组质粒进行序列测定,并对等位基因进行标准命名；以等位基因重组质粒为模板制备等位基因分型标准物。 结果 D11S4463基因座和D17S974基因座在中国汉族人群中具有较高的遗传多态性,分别检测出9个和8个等位基因,其观察杂合度分别为0.735和0.747；匹配概率分别为0.089和0.115；个体识别能力分别为0.911和0.885；多态性信息含量分别为0.73和0.70；非父排除概率分别为0.485和0.505。应用分子克隆方法成功构建了D11S4463基因座和D17S974基因座等位基因分型标准物。 结论 D11S4463基因座和D17S974基因座具有较高的遗传多态性,是一个适合于法医学和群体遗传学研究的遗传标记,应用分子克隆的方法构建其等位基因分型标准物是一种实现其等位基因分型标准化的可行办法。
[Abstract]:Objective to investigate the genetic polymorphism of D11S4463 locus and D17S974 locus in Chinese Han population and to prepare their alleles by molecular cloning. Methods the genetic polymorphisms of D11S4463 and D17S974 loci were investigated by fluorescence PCR and ABI3130XL genetic analyzer in 600 unrelated individuals of Han nationality in China, and their alleles were constructed by molecular cloning. At first, 9 alleles of D11S4463 locus and 8 alleles of D17S974 locus were screened out in the population. The purified products were amplified by PCR and transformed into DH5 伪 competent Escherichia coli by ligation with pMD18-T plasmid vector. The allelic recombinant plasmids were sequenced and the alleles were named as standard alleles, and the allelic genotyping standard compounds were prepared by using allelic recombinant plasmids as template. Results D11S4463 locus and D17S974 locus were highly polymorphic in Chinese Han population, 9 and 8 alleles were detected respectively, the observed heterozygosity were 0.735 and 0.747, the matching probability were 0.089 and 0.115, respectively. The recognition ability of individuals was 0.911 and 0.885, the polymorphic information content was 0.73 and 0.70, and the non-paternal exclusion probability was 0.485 and 0.505, respectively. The alleles of D11S4463 locus and D17S974 locus were successfully constructed by molecular cloning. Conclusion D11S4463 locus and D17S974 locus are highly polymorphic, which is a suitable genetic marker for forensic and population genetics research. It is a feasible method to construct its allelic genotyping standard by molecular cloning.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：D919.4
【参考文献】
相关期刊论文 前10条
1 袁丽;鲁涤;石美森;杨雪;;岫岩满族9个STR基因座遗传多态性[J];证据科学;2011年05期
2 张林,辛军平,廖淼,陈国弟,李荣华,吴梅筠;用分子克隆技术构建D12S375基因座等位基因分型标准物及汉、回、维族群体遗传学研究[J];中国法医学杂志;2001年04期
3 陈慧,余纯应,杨庆恩,杨荣芝,梅q ,徐雯;STR等位基因梯阶制备的研究[J];中国法医学杂志;2002年04期
4 胡慧英;刘超;杨电;刘长晖;;两无关个体单亲亲子鉴定不排除1例[J];中国法医学杂志;2006年05期
5 陈玲;侯庆唐;王冬花;;三联体亲子鉴定1例[J];中国法医学杂志;2008年01期
6 巴华杰;罗斌;刘冰泉;林子清;马骏;朱爱华;;DNA数据库9个STR基因座比中认定亲权的可靠性分析[J];中国法医学杂志;2008年04期
7 白雪;丛斌;李淑瑾;谷建立;侯志平;刘宁;李霞;;河北汉族群体D17S974和D11S4463 miniSTR遗传多态性[J];中国法医学杂志;2009年03期
8 邵伟波;张素华;李莉;;21个非CODIS STR基因座的遗传多态性[J];法医学杂志;2011年01期
9 李生斌,郑海波,冯继东,于嘉琳;中华民族STR遗传结构及变化规律的研究(Ⅰ)[J];西安医科大学学报(中文版);2000年01期
10 赖江华,张保华,朱波峰,陈腾,郑海波,寻兮,李生斌;云南白族STR遗传多态性研究[J];西安交通大学学报(医学版);2002年03期
，本文编号：2066708
本文链接：https://www.wllwen.com/yixuelunwen/yundongyixue/2066708.html
