基于SRY基因的人血迹性别快速鉴定的方法学研究

发布时间：2018-06-25 23:32

本文选题：性别快速鉴定 + 二温度点PCR法　；参考：《贵阳中医学院学报》2016年03期

【摘要】：目的:在案发现场快速准确的锁定犯罪嫌疑人性别,缩小嫌疑人范围,为基层公安机关案件侦查提供技术支持。方法:通过对碱裂解法进行改良并建立人类凝固血液的基因组DNA提取方法;根据Y染色体的SRY基因序列设计特异性引物,优化PCR扩增反应条件,扩增产物经SYBR Green I染色后紫外光下观察。结果:改良碱裂解法中组2所得DNA浓度为(271.08±143.28)ng/μL,显著优于组1所得(P0.05),且经ITS序列验证均能扩增出目的条带;在PCR扩增产物加入SYBR GreenⅠ染料后于紫外光下观察,男性血样的PCR扩增产物呈现绿色荧光,而女性血样的PCR扩增产物未有荧光。结论:使用改良碱裂解法中的组2快速提取案发现场凝血DNA,进行特异性PCR扩增后,观察加入SYBR Green I后的PCR产物紫外光下是否有绿色荧光即能判断性别,该方法准确、可靠,较常规方法缩短1~2 h。
[Abstract]:Objective: to quickly and accurately locate the gender of criminal suspects, narrow the scope of suspects, and provide technical support for the investigation of cases in grass-roots public security organs. Methods: the genomic DNA extraction method of human coagulated blood was established by modified alkaline lysis method, and specific primers were designed according to the SRY gene sequence of Y chromosome to optimize the reaction conditions of PCR amplification. The amplified products were stained by SYBR Green I and observed under ultraviolet light. Results: the concentration of ng/ in group 2 was (271.08 卤143.28) ng/ 渭 L, which was significantly higher than that in group 1 (P0.05). The PCR products of male blood samples showed green fluorescence, while those of female blood samples did not. Conclusion: group 2 of modified alkaline lysis method was used to extract the coagulated DNA in the field of crime, and the specific PCR amplification was carried out. The results showed that the green fluorescence of the PCR product after adding SYBR Green I could determine the sex. The method was accurate and reliable. Compared with the conventional method, it shortened 1 hour by 2 hours.
【作者单位】：贵阳中医学院;贵阳市公安局刑侦支队;
【基金】：贵州省科研厅科技计划;项目编号:黔科合SY[2013]3109号贵州省普通高等学校特色重点实验室项目;项目编号:黔教合KY字[2013]108号
【分类号】：D919.4

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