1800MHz电磁辐射对不同日龄仔鼠海马区神经细胞增殖的影响

发布时间：2018-06-27 01:18

本文选题：电磁辐射 + 脑　；参考：《郑州大学》2017年硕士论文

【摘要】：1背景和目的随着电子技术的应用和通讯工业的迅速发展,各种电子设备、无线通讯设施广泛应用于日常生活和工作中,环境中的电磁辐射(Electromagnetic radiation)也日渐增多,甚至有人称其为的第四大环境公害。处于生长发育期的儿童同样不可避免的暴露于各种频率和强度的电磁辐射中。有关电磁辐射对中枢神经系统功能的影响研究越来越多,但目前对电磁辐射对大脑影响研究主要集中在对成熟脑组织影响上,发现移动电话产生的电磁辐射可导致成年小鼠血脑屏障通透性增加,神经递质代谢紊乱,皮层及海马出现组织学和超微结构损伤,如神经元缺失、细胞排列紊乱,胞浆尼氏体减少、核内出现透明区等早期凋亡等一系列征象。但对于不成熟脑组织研究较少,有研究表明机体器官组织在发育期对电磁场的影响尤其敏感,而其中又以相对不成熟的脑最易受影响,如对记忆、学习能力和神经行为能力的影响成为国内外学者日益关注的焦点。由于电磁辐射神经生物学损伤机制的复杂性,所以电磁辐射对处于不同发育成熟度脑组织影响的是否存在差别,目前尚无明确结论。本研究选用7日龄、21日龄小鼠,从脑发育成熟度分别相当于新生儿和青少年期;通过模拟手机1800MHz电磁辐射暴露环境,研究短期电磁辐射暴露对不同发育成熟度小鼠脑组织海马区神经细胞增生的影响,为防治电磁辐射所致发育期脑损伤提供理论依据。2材料与方法将7日龄(P7)和21日龄(P21)昆明小鼠随机分为辐射组和对照组,每组12只,共48只。实验通过GTEM Cell(GHz横电磁波室)模拟手机辐射环境,频率选取1800MHz,场强为28V/m,功率密度为2.0m W/cm2,小鼠置于电磁波室内,每天8h,一次4小时,一天2次,中间间隔1h,连续暴露3d。对照组给予虚拟假辐射,辐射组动物分别于辐射前1h腹腔注射Brd U50mg/Kg,对照组连续给予等体积的生理盐水腹腔注射,连续腹腔注射3天。各组小鼠在最后一次腹腔注射24h后,经无水乙醚吸入麻醉,灌注生理盐水和甲醛溶液至躯体苍白变硬,快速分离并取出脑组织,制备海马层面脑组织切片,采用免疫组织化学法检测海马齿状回(dentate gyrus,DG)颗粒细胞下层Caspase-3、Brd U、Ki67、BLBP、HH3的表达。3结果3.1电磁辐射对不同日龄小鼠海马齿状回颗粒下层(SGZ)神经细胞Caspase-3表达的影响普通光学显微镜200倍时镜下观察发现:P7及P21小鼠短期暴露于1800MHz电磁辐射后海马齿状回颗粒下层几乎没有发现Caspase-3阳性细胞表达。与对照组相比,辐射组海马齿状回区结构疏松,神经细胞排列松散、紊乱。3.2电磁辐射对不同日龄小鼠脑组织海马齿状回SGZ区Brd U、Ki67表达的影响与对照组相比,P7辐射组小鼠脑组织海马齿状回SGZ区Brd U、Ki67阳性细胞表达明显增多,差异有统计学意义(P0.05);P21辐射组小鼠与对照组相比,海马齿状回SGZ区Brd U、Ki67阳性细胞表达几乎无变化,差异无统计学意义(P0.05)。3.3电磁辐射对不同日龄小鼠脑组织海马齿状回SGZ区BLBP、HH3表达的影响P7小鼠暴露于电磁辐射后BLBP和HH3的阳性细胞表达减少,差异有统计学意义(P0.05);而P21辐射组小鼠与对照组相比,BLBP和HH3阳性细胞表达几乎无变化。4结论1800MHz电磁辐射对不同成熟度小鼠脑组织海马DG区神经细胞均未引起凋亡,对发育相对不成熟脑组织有促进神经细胞增生作用,但对发育相对成熟的脑组织却无明显影响。
[Abstract]:1 background and purpose with the application of electronic technology and the rapid development of communication industry, all kinds of electronic equipment and wireless communication facilities are widely used in daily life and work. The electromagnetic radiation (Electromagnetic radiation) in the environment is increasing, and even the fourth environmental hazards, which are called it, are in the same growing period of children. The influence of electromagnetic radiation on the function of central nervous system is more and more, but the influence of electromagnetic radiation on the brain is mainly focused on the mature brain tissue, and it is found that the electromagnetic radiation produced by mobile phone can lead to the blood brain screen of adult mice. An increase in barrier permeability, disorder of neurotransmitter metabolism, histology and ultrastructural damage in the cortex and hippocampus, such as neuron loss, disorder of cell arrangement, cytosolic Nissl body reduction, early apoptosis in the nucleus, and so on. However, there are few studies on immature brain tissue. The influence of electromagnetic field is especially sensitive, and the relatively immature brain is most susceptible to the influence of the brain, such as memory, learning ability and neurobehavioral ability, which has become the focus of attention of scholars at home and abroad. The electromagnetic radiation is in the shadow of different mature brain tissues because of the complexity of the mechanism of neurobiological damage. In this study, 7 days of age and 21 day old mice were selected as the equivalent of the newborn and adolescence in the 7 days of age and 21 days of age. By simulating the exposure environment of the electromagnetic radiation of the mobile phone, the short-term electromagnetic radiation exposure was used to study the proliferation of neurons in the hippocampus of the brain tissues of different mature mice. In order to provide theoretical basis for the prevention and control of brain damage induced by electromagnetic radiation,.2 materials and methods were used to randomly divide the 7 days old (P7) and 21 day old (P21) Kunming mice into radiation and control groups, with 12 rats in each group, with a total of 48. The experiments were carried out through the GTEM Cell (GHz transverse electromagnetic wave chamber) to simulate the radiation environment of the cell phone, the frequency selected 1800MHz, the field strength 28V/m, and the power density. For 2.0m W/cm2, the mice were placed in the electromagnetic wave room, 8h every day, 4 hours a day, 2 times a day, and 1H in the middle interval. The 3D. control group was continuously exposed to virtual false radiation. The radiation group was injected with Brd U50mg/Kg before the radiation of 1H, and the control group was given the equal volume of physiological salt water intraperitoneally for 3 days. The mice in each group were at the end. After an intraperitoneal injection of 24h, inhalation of anhydrous ether, perfusion of saline and Formaldehyde Solution to the body and hard, quickly separating and removing brain tissue, preparing hippocampal slice of brain tissue, using immunohistochemical method to detect Caspase-3, Brd U, Ki67, BLBP, HH3.3 of the dentate gyrus, DG granulosa cells Results the effect of 3.1 electromagnetic radiation on the expression of Caspase-3 expression in the dentate gyrus sublayer (SGZ) neurons of different days of age of mice was 200 times more than that of the ordinary optical microscope. It was found that the P7 and P21 mice were exposed to the 1800MHz electromagnetic radiation of the Houhai dentate gyrus and the Caspase-3 positive cells were hardly found in the 1800MHz electromagnetic radiation. Compared with the control group, the effects of.3.2 electromagnetic radiation on the expression of Brd U and Ki67 in the SGZ region of hippocampus dentate gyrus of mice with different days of age were compared with those of the control group. The expression of Brd U and Ki67 positive cells in the hippocampal dentate gyrus of the mice of P7 radiation group increased significantly, and the difference was statistically significant (the difference was statistically significant). The expression of Brd U and Ki67 positive cells in SGZ area of hippocampal dentate gyrus was almost unchanged, and there was no statistical difference (P0.05).3.3 electromagnetic radiation on BLBP and HH3 expression in the SGZ region of hippocampal dentate gyrus of mice with different days of age (P0.05), and the expression of positive cells decreased after exposure to electromagnetic radiation in P21 radiation group (P0.05). The difference was statistically significant (P0.05), while the P21 radiation group was compared with the control group, the expression of BLBP and HH3 positive cells almost did not change.4 conclusion 1800MHz electromagnetic radiation did not induce apoptosis in the hippocampal DG region nerve cells in the brain tissues of the mice with different maturity. The relatively mature brain tissue has no obvious effect.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R818

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