有氧运动对2型糖尿病大鼠肾脏VEGF-A及VEGFR2蛋白表达的影响

发布时间：2018-06-28 10:41

本文选题：大鼠 + 型糖尿病　；参考：《上海体育学院学报》2015年06期

【摘要】：目的:研究有氧运动对2型糖尿病大鼠肾脏VEGF-A、VEGFR2蛋白表达的影响,并对其可能机制进行探讨。方法:通过高糖高脂饲喂5周加低剂量腹腔注射STZ复制2型糖尿病大鼠模型,7周后成模SD大鼠被随机分为糖尿病安静组和糖尿病运动组,另设正常对照组。采用7周有氧游泳运动只对糖尿病运动组进行干预,实验末测定血糖、24 h UA、肾皮质VEGF-A及VEGFR2和p-VEGFR2蛋白的表达及光镜、电镜观察肾小球形态结构的变化。结果:光镜下糖尿病运动组大鼠肾小球增大不明显,毛细血管袢大多数开放,球囊壁渗出、粘连等现象较糖尿病安静组明显改善;基底膜无明显增厚,足突融合等现象较糖尿病安静组均有所减轻;血糖浓度和24 h UA排泄量降低显著(P0.05或P0.01);肾皮质VEGF-A、VEGFR2和p-VEGFR2蛋白的表达均较糖尿病安静组显著降低(P0.05或P0.01)。结论:运动对2型糖尿病大鼠肾脏有保护作用,其作用机制可能是通过其降低血糖及下调2型糖尿病状态下表达增加的VEGF-A和VEGFR2蛋白水平,而减轻大鼠肾脏损伤。
[Abstract]:Aim: to study the effect of aerobic exercise on the expression of VEGF-An VEGFR2 protein in kidney of type 2 diabetic rats and to explore its possible mechanism. Methods: SD rats were randomly divided into two groups: diabetes quiet group and diabetic exercise group after 5 weeks of high glucose and high fat diet and low dose intraperitoneal injection of STZ to induce type 2 diabetic rats. Seven weeks aerobic swimming exercise was used only to intervene in diabetic exercise group. At the end of the experiment, 24 hours UAA, VEGF-A, VEGFR2 and p-VEGFR2 protein expression in renal cortex and light microscope were measured. The changes of glomerular morphology were observed by electron microscope. Results: the glomerular enlargement was not obvious, the capillary loops were mostly open, the balloon wall exudated, adhesion and other phenomena were significantly improved in the diabetic exercise group compared with the quiet diabetic group, the basement membrane was not significantly thickened. Compared with the quiet diabetic group, the phenomenon of foot process fusion was alleviated, the concentration of blood glucose and 24 h UA excretion decreased significantly (P0.05 or P0.01), and the expression of VEGF-An VEGFR2 and p-VEGFR2 protein in renal cortex were significantly lower than those in the quiet diabetic group (P0.05 or P0.01). Conclusion: exercise has protective effect on the kidney of type 2 diabetic rats. The mechanism of exercise may be to reduce the renal injury by lowering blood glucose and down-regulating the increased VEGF-A and VEGFR2 protein levels in type 2 diabetes mellitus.
【作者单位】：井冈山大学体育学院;华南师范大学体育科学学院;
【基金】：国家自然科学基金资助项目(31360255,31160217) 井冈山大学自然科学研究项目(JZB1313)
【分类号】：R875

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