椭圆形骨道改良重建兔前交叉韧带动物模型的建立

发布时间：2018-06-29 19:27

本文选题：椭圆形骨道 + 前交叉韧带　；参考：《中国运动医学杂志》2017年04期

【摘要】：目的:解剖研究兔前交叉韧带止点的大体形态,建立椭圆形骨道改良重建兔前交叉韧带动物模型。方法:18只新西兰大白兔(2.5~3 kg),8只用于前交叉韧带止点解剖研究,10只用于椭圆形骨道动物模型的建立。去除兔膝关节股骨内髁和前交叉韧带周围软组织,观察兔前交叉韧带股骨和胫骨止点形态,并使用游标卡尺对止点长短径进行测量。制作椭圆形骨道扩孔器(截面1.6 mm×2.5 mm),对10只新西兰大白兔右腿行椭圆形骨道重建前交叉韧带作为椭圆形骨道组,对其左腿用传统方法圆形骨道(截面直径2 mm)重建前交叉韧带作为圆形骨道组。取兔自体半腱肌放入骨道并固定,观察移植物与骨道的匹配程度。术后立即对兔双侧膝关节行三维CT扫描,对股骨和胫骨骨道的截面积、长径、短径、直径进行测量。结果:兔前交叉韧带止点的解剖形态为椭圆形。股骨止点的长径为5.28±0.83 mm,短径为2.61±0.33 mm。胫骨止点长径为5.33±0.40 mm,短径为2.68±0.11 mm。移植物肌腱与骨道匹配良好,未见明显空隙。椭圆形骨道组股骨骨道截面积为3.18±0.09 mm~2,胫骨骨道截面积为3.26±0.15 mm~2。圆形骨道组股骨骨道截面积为3.13±0.10 mm~2,胫骨骨道截面积为3.11±0.11 mm~2。椭圆形骨道组和圆形骨道组之间骨道截面积无显著差异。结论:兔前交叉韧带止点为椭圆形。使用自制椭圆形骨道扩孔器可成功构建与圆形骨道相对应的兔椭圆形骨道动物模型,并有很好的移植物与骨道匹配性,为下一步深入研究奠定了实验动物模型基础。
[Abstract]:Objective: to study the morphology of anterior cruciate ligament (ACL) in rabbits and to establish an animal model of anterior cruciate ligament (ACL) reconstruction. Methods Twenty eight New Zealand white rabbits (2.5kg) were used for anatomic study of anterior cruciate ligament (ACL) insertion. 10 rabbits were used to establish the animal model of the oval osseous canal. The soft tissue around the medial condyle and anterior cruciate ligament of the knee joint was removed to observe the shape of femoral and tibial insertion points of the anterior cruciate ligament and to measure the length and diameter of the insertion point by Vernier caliper. An elliptical osseous canal reaming apparatus was made (cross section 1.6 mm 脳 2.5 mm), for reconstruction of anterior cruciate ligament (ACL) in 10 New Zealand white rabbits with right leg as oval osseous canal group). The anterior cruciate ligament (ACL) of the left leg was reconstructed by the traditional method of circular osseous canal (2 mm cross-sectional diameter) as the round osseous canal group. The autologous semitendinosus muscle was taken into the bone canal and fixed to observe the matching degree between the graft and the bone canal. Three dimensional CT scanning was performed on bilateral knee joints immediately after operation. The sectional area, long diameter, short diameter and diameter of femur and tibial bone canal were measured. Results: the anatomic shape of the anterior cruciate ligament was oval. The length and short diameters of femoral terminations were 5.28 卤0.83 mm and 2.61 卤0.33 mm, respectively. The length and the short diameter of tibia were 5.33 卤0.40 mm and 2.68 卤0.11 mm respectively. The graft tendon matched well with the bone canal and there was no obvious gap. The cross sectional area of femur and tibia were 3.18 卤0.09 mm and 3.26 卤0.15 mm / 2 in oval group. The cross sectional area of femur and tibia were 3.13 卤0.10 mm and 3.11 卤0.11 mm / 2 respectively. There was no significant difference in the cross-sectional area between the oval and round bone passage groups. Conclusion: the insertion point of anterior cruciate ligament is oval. The animal model of rabbit oval bone canal corresponding to circular bone canal can be successfully constructed by using self-made elliptical bone canal reaming device, and it has good matching between graft and bone tract, which lays the foundation for further study of experimental animal model.
【作者单位】：北京大学第三医院运动医学研究所;北京市运动医学关节伤病重点实验室;
【基金】：北京市科技新星计划[项目编号:xx2014003(A)]
【分类号】：R-332;R873

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