人参皂甙Rg1对力竭运动大鼠骨骼

发布时间：2018-07-04 12:01

  本文选题：运动性疲劳 + 自由基　； 参考：《湖南师范大学》2016年硕士论文

【摘要】：1研究目的本实验结合自由基疲劳理论和羰基毒化的研究成果,采用力竭疲劳模型,观察4周中等强度运动、8周中等强度运动及各自结合灌胃人参皂甙Rg1,对力竭状态大鼠骨骼肌和脑组织羰基化蛋白含量的影响。从去羰基应激的角度,初步探讨人参皂Rg1抗运动性疲劳的作用机制。2研究方法实验对象为清洁级SD大鼠40只,随机分为4周安静组(Q4)、4周运动组(E4)、4周安静给药组(QR4)、4周运动给药组(ER4)、8周安静组(Q8)、8周运动组(E8)、8周安静给药组(QR8)、8周运动给药组(ER8),每组5只。运动给药组和安静给药组大鼠按照30mg/kg体重剂量灌胃人参皂甙Rg1溶液,运动组和安静组大鼠灌胃等容积生理盐水。运动组与运动给药组大鼠接受训练周期分别为4周和8周,负荷为25m·min-1×30min的水平跑台运动训练。运动方案结束后,记录力竭时间,并即刻取材。测定大鼠血清中血乳酸(LA)含量;测定大鼠骨骼肌和脑组织中超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)含量以及羰基化蛋白的含量;3研究结果3.1体重测定结果实验后,各组SD大鼠体重均显著增加;E4组与ER4组均显著低于QR4组(P0.05);E8组和ER8组显著低于Q8组(P0.05)、QR8组(P0.01);其余均无显著性差异。3.2力竭时间测定结果与Q4组比较,E4组和ER4组显著延长(P0.01);与QR4组比较,ER4组显著延长(P0.01);与E4组比较,ER4组力竭时间显著延长(P0.01)。与Q8组比较,E8组和ER8组显著延长(P0.01);与QR8组比较,ER8组显著延长(P0.01)。与E8组比较,ER8组显著延长(P0.01)。E8组力竭时间显著长于E4组(P0.01),ER8组力竭时间显著长于ER4组(P0.01)。其余均无显著性差异。3.3血乳酸测定结果与Q4组比较,E4组和ER4组显著降低(P0.01);与QR4组比较,ER4组显著降低。与Q8组比较,E8组和ER8组显著降低(P0.01);与QR8组比较,ER8组显著降低(P0.01)。其余均无显著性差异。3.4大鼠骨骼肌中抗氧化酶活性、MDA含量、羰基化蛋白含量与Q4比较,E4组CAT活性显著升高(P0.05),MDA含量、羰基化蛋白含量显著降低(P0.01);与QR4组比较,ER4组SOD活性、GSH-Px活性和CAT活性显著升高(P0.05),MDA含量、羰基化蛋白含量显著降低(P0.05);与E4组比较,ER4组SOD活性显著升高(P0.05),MDA含量显著降低(P0.05);与Q8组比较,E8组GSH-Px活性和CAT活性显著升高(P0.01,P0.05),MDA含量、羰基化蛋白含量显著降低(P0.01);与QR8组比较,ER8组SOD活性、GSH-Px活性和CAT活性显著升高(P0.01),MDA含量、羰基化蛋白含量显著降低(P0.01);与E8组比较,ER8组SOD活性(P0.01)、GSH-Px活性和CAT活性显著升高(P0.05),MDA含量和羰基化蛋白含量显著降低(P0.05)。ER8组SOD活性显著高于ER4组(P0.05);在GSH-Px活性方面,E8组显著高于E4组(P0.05),ER8组显著高于ER4组(P0.05)。其余均无显著性差异。3.5大鼠脑组织中抗氧化酶活性、MDA含量、羰基化蛋白含量与Q4组比较,E4组羰基化蛋白含量显著降低(P0.01)。与QR4组比较,ER4组SOD活性和CAT活性显著升高(P0.01,P0.05),MDA含量、羰基化蛋白含量显著降低(P0.05,P0.01);与E4组比较,ER4组SOD活性显著升高(P0.01)。与Q8组比较,E8组SOD活性(P0.01)、GSH-Px活性(P0.01)和CAT活性(P0.05)显著升高,MDA含量、羰基化蛋白含量显著降低(P0.01)。与QR8组比较,ER8组SOD活性、GSH-Px活性和CAT活性显著升高(P0.01),MDA含量、羰基化蛋白含量显著降低(P0.01)。与E8组比较,ER8组SOD活性、GSH-Px活性显著升高(P0.01,P0.05),MDA含量和羰基化蛋白含量显著降低(P0.05)。ER8组SOD活性、GSH-Px活性、CAT活性均显著高于ER4组(P0.05);ER8组MDA含量显著低于ER4组(P0.05)。其余均无显著性差异。4结论4.1长期运动训练可引起大鼠机体抗氧化酶活性适应性增强,提高机体对抗氧化应激和羰基应激能力,减少机体体内羰基化蛋白的生成。4.2人参皂甙Rg1可引起运动大鼠机体抗氧化酶活性适应性增强,提高机体对抗氧化应激和羰基应激能力,减少机体体内羰基化蛋白的生成。4.3运动训练及结合人参皂甙Rg1均可提高大鼠运动能力,延长力竭运动时间。
[Abstract]:1 the purpose of this study was to combine the results of free radical fatigue theory and carbonyl toxicity, using exhaustive fatigue model, to observe the 4 week moderate intensity exercise, 8 weeks of moderate intensity exercise and the effect of each combination of gavage ginsenoside Rg1 on the carbonylation of egg white in the skeletal muscle and brain tissue of the exhausted rats. The effect mechanism of ginseng soap Rg1 on exercise fatigue resistance was studied by.2, which were divided into 40 clean grade SD rats, randomly divided into 4 weeks quiet group (Q4), 4 week exercise group (E4), 4 week quiet administration group (QR4), 4 week exercise group (ER4), 8 week quiet group (Q8), 8 week exercise group (E8), 8 weeks' quiet administration group (QR8), 8 week exercise group (ER8), 5 each 5 The rats in the exercise group and the quiet administration group were given the Rg1 solution of ginsenoside according to the 30mg/kg weight dose, the exercise group and the quiet group rats were filled with saline. The training period of the exercise group and the exercise group was 4 weeks and 8 weeks respectively, the load was 25m min-1 x 30min, and the exercise program was finished, after the exercise program was finished, The content of serum lactate (LA) in rat serum was measured and the content of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) content and carbonylation protein in the skeletal muscle and brain tissue of rats were measured, and the results of 3 study results of 3.1 body weight test were tested. The weight of SD rats in group E4 and ER4 group was significantly lower than that of group QR4 (P0.05), and E8 and ER8 groups were significantly lower than group Q8 (P0.05) and QR8 group (P0.01). The 4 groups were significantly prolonged (P0.01). Compared with the Q8 group, the E8 group and the ER8 group were significantly prolonged (P0.01). Compared with the QR8 group, the ER8 group was significantly longer (P0.01). The ER8 group was significantly longer than the E8 group (P0.01) and the duration of the exhaustion of the.E8 group was significantly longer than that of the group. Compared with group Q4, group E4 and group ER4 significantly decreased (P0.01). Compared with group QR4, ER4 group decreased significantly. Compared with group Q8, E8 group and ER8 group decreased significantly (P0.01). Compared with QR8 group, ER8 group significantly decreased. Group CAT activity increased significantly (P0.05), MDA content and carbonylation protein content decreased significantly (P0.01). Compared with QR4 group, SOD activity in group ER4, GSH-Px activity and CAT activity increased significantly (P0.05), MDA content, and carbonylation protein content significantly decreased (P0.05). Compared with group E8, the activity of GSH-Px and CAT activity increased significantly (P0.01, P0.05), MDA content, and carbonylation protein content significantly decreased (P0.01). Compared with QR8 group, SOD activity of ER8 group, GSH-Px activity and CAT activity increased significantly, and the content of carbonylation protein decreased significantly. The content of MDA and carbonylation protein decreased significantly (P0.05), and the activity of SOD in group.ER8 was significantly higher than that in group ER4 (P0.05). In GSH-Px activity, the E8 group was significantly higher than the E4 group (P0.05), and the ER8 group was significantly higher than that of the group (P0.05). Compared with group Q4, the content of carbonylation protein in group E4 decreased significantly (P0.01). Compared with group QR4, SOD activity and CAT activity in ER4 group increased significantly (P0.01, P0.05), MDA content, and carbonylation protein content significantly decreased (P0.05, P0.01). CAT activity (P0.05) increased significantly, MDA content and carbonylation protein content decreased significantly (P0.01). Compared with QR8 group, SOD activity in group ER8, GSH-Px activity and CAT activity increased significantly (P0.01), MDA content, and carbonylation protein content significantly decreased (P0.01). The protein content significantly decreased (P0.05).ER8 group SOD activity, GSH-Px activity, CAT activity was significantly higher than ER4 group (P0.05), ER8 group MDA content was significantly lower than ER4 group (P0.05). The rest no significant difference in.4 conclusion 4.1 long-term exercise training can cause the adaptation to antioxidant enzyme activity in rats, improve the body against oxidative stress and carbonyl stress energy. Force, reducing the formation of.4.2 ginsenoside Rg1 in the body of body carbonylation protein can cause the activity of anti oxidative stress and carbonyl stress in the body of the exercise rats, reduce the activity of carbonylation of protein in the body and reduce the.4.3 exercise training and the combination of human ginseng saponins Rg1, which can increase the exercise ability of rats and prolong the exercise ability of rats. Exhausting exercise time.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R87
，

本文编号：2096013