代谢组学运动选材与传统生化选材方法的比较研究

发布时间：2018-07-11 11:01

  本文选题：代谢组学 + 运动选材　； 参考：《江西师范大学》2012年硕士论文

【摘要】：研究背景与目的：在后基因时代，以系统整体为研究对象，以基因组学、蛋白质组学和代谢组学为代表的系统生物学已日渐成为生命科学研究的主流趋势。代谢组学通过测量样本中所有的代谢产物来反映生物体对不同刺激的反应，，利用代谢指纹图谱对生命系统进行研究。相对于基因层面的研究，代谢组学具有灵敏性更高，整体性更强，测试与分析简单、快速等优点，在此前的研究中，我们已初步证明了运动员的代谢指纹图谱包含了运动员竞技水平的信息，并建立了高水平男子中短距离游泳成绩预测代谢组学模型。本研究以SD大鼠为研究对象，探索能否利用大鼠幼年时（4周龄）的代谢指纹图谱，识别出游泳潜力较大的大鼠，即经过长期训练后，在成年时（14周龄），能取得优秀游泳成绩的大鼠；并通过与传统生化方法进行对比，对基于核磁共振（NMR）的代谢组学这一系统生物学方法应用于运动选材的可行性与优越性进行分析。 研究方法：以24只SD大鼠为研究对象，在大鼠4周龄时收集它们的尿样和血样，尿样用于核磁共振（NMR）代谢组学分析，血样用于测试血红蛋白（Hb）和血清睾酮(ST)；然后进行为期10周的渐增负荷游泳训练，训练结束后进行负重耐力游泳能力测试，记录每只大鼠游泳至力竭的时间，并根据力竭时间长短进行排名，分为前3名组与4-24名组、前12名组与后12名组；测试完成后，休息两天，断头处死，取右后肢腓肠肌测试琥珀酸脱氢酶（SDH）和乳酸脱氢酶（LDH）的含量。 研究结果：前3名组和4-24名组大鼠的Hb、ST、SDH、LDH分别为：152.3±9.5:165.73±74.42g/L、2.47±0.06:2.48±0.34nmol/L、29.77±7.48:35.38±38.23U/mgprot、0.58±0.4:0.62±0.29U/mgprot，没有显著性差异；前12名组和13-24名组大鼠的Hb、ST、SDH、LDH分别为：153.72±21.72:174.39±65.76g/L、2.47±0.33:2.49±0.13nmol/L、33.46±40.05:35.9±37.72U/mgprot、0.59±0.39:0.64±0.27U/mgprot，结果同样没有显著性差异；代谢组学模式识别结果显示，前3名组和4-24名组样本、前12名组和后12名组的代谢指纹图谱有了明显的差异，各组样本被明确地分开了，而同组的样本则出现了聚集。 研究结论：幼年大鼠尿液的代谢指纹图谱能一定程度反映大鼠的游泳运动潜力的差异，相对于利用个别或者少数几个指标进行运动选材的传统生化方法，代谢组学这一系统生物学方法具有其独特的优势。
[Abstract]:Background & objective: in the post-gene era, the system biology, which takes the whole system as the research object and the genomics, proteomics and metabolomics as the representative, has become the main trend of life science research. Metabonomics measures all the metabolites in the samples to reflect the response of organisms to different stimuli, and studies the life system by using metabolic fingerprints. Metabonomics is more sensitive, more holistic, easier to test and analyze, and faster than research at the gene level. We have preliminarily proved that the metabolic fingerprint of athletes contains the information of athletes' competitive level and established a metabolic model for predicting the performance of high level men in short distance swimming. In this study, SD rats were chosen as research subjects to explore whether the metabolic fingerprints of young rats (4 weeks old) could be used to identify the rats with great swimming potential, that is, after long-term training, In adulthood (14 weeks of age), rats with excellent swimming performance were compared with traditional biochemical methods. The feasibility and superiority of nuclear magnetic resonance (NMR) based metabonomics, a systematic biological method, in sports material selection were analyzed. Methods: urine and blood samples were collected from 24 SD rats at the age of 4 weeks. Urine samples were used for nuclear magnetic resonance (NMR) metabonomics analysis, blood samples were used to test hemoglobin (HB) and serum testosterone (St). After the training, the endurance swimming ability was tested. The time of swimming to exhaustion of each rat was recorded and ranked according to the time of exhaustion, which was divided into the top 3 groups and 4-24 groups. The first 12 groups and the latter 12 groups, after the test, rest for two days, then execute the head, take the right hind limb gastrocnemius muscle to test the content of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH). 鐮旂┒缁撴灉锛氬墠3鍚嶇粍鍜

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