牵张训练对腓肠肌拉伤大鼠损伤修复的影响研究
发布时间：2018-07-15 10:12
【摘要】：实验目的:1、通过对腓肠肌急性拉伤后不同时间点组织切片的观察,明确腓肠肌拉伤后组织结构的修复过程。2、通过观察牵张、牵张+冰敷干预后腓肠肌急性拉伤后不同时间点组织切片的修复过程,明确腓肠肌损伤后的科学处理方法。3、通过观察牵张与牵张+冰敷治疗对损伤骨骼肌COL-I蛋白表达的影响,进一步探讨牵张与牵张+冰敷对腓肠肌急性拉伤后的作用效果及机理。实验方法:选取雄性SD大鼠64只,随机分为正常大鼠组A组、损伤即刻B组、自然恢复组C_1组、自然恢复组C_2组、牵张组D_1组、牵张组D_2组、牵张+冰敷组E_1组、牵张+冰敷组E_2组。除正常组外,其余56只大鼠均建立腓肠肌拉伤模型。实验模型制作完成后,D_1组、D_2组在模型建立12天后开始采用牵张手法进行康复,每天固定时间、人员操作。E_1组、E_2组在实验模型建立12天后采用牵张手法康复,然后进行冰敷,每天固定时间、人员操作。其余组正常喂养。实验各组在相应的时间进行腓肠肌取材,并制作石蜡切片观察并测试相关指标。实验结果:1、大鼠腓肠肌光镜观察结果:拉伤一小时,在光镜切片下观察,受损部位肌纤维组织结构不整齐,同时伴随出现水肿的情况,肌纤维之间的间隙情况不一致,有的明显加大,可观察到存在炎症反应,并可见血管扩张发生。在骨骼肌受损后18天组织切片观察可见,自然恢复组受损部位肌纤维出现修复,但没有完全修复,肌纤维存在大小不一,排列不够整齐。牵张组与牵张+冰敷组治疗后,受损部位新生的肌纤维排列更加整齐,结构更加完整。在骨骼肌受损后25天光镜观察可见,自然恢复组受损部位肌纤维出现修复,修复存在瘢痕,肌纤维存在大小不一,排列不够整齐。牵张组与牵张+冰敷组治疗后,受损部位新生的肌纤维基本排列整齐,修复较好。2、大鼠腓肠肌胶原蛋白COL-I免疫组化测试结果:损伤一小时,COL-I蛋白表达随即下降,在损伤18天自然恢复组C1、牵张组D_1、牵张+冰敷组E_1胶原蛋白COL-I蛋白表达均出现最大值,自然恢复组B_1组与正常组存在极显著性差异(P0.01),与损伤即刻组B组有极显著性差异(P0.01),牵张组D_1组、牵张+冰敷组E_1组与损伤即刻B组、正常组COL-I蛋白表达均存在显著性差异(P0.05)。在损伤25天自然恢复组C_2组与正常组存在显著性差异(P0.05),牵张组D_2组、牵张+冰敷组E_2组与正常组COL-I蛋白表达均无显著性差异。牵张组D_1牵张+冰敷组E_1无显著性差异,牵张组D_2牵张+冰敷组E_2无显著性差异。牵张组D_1与牵张组D_2存在显著性差异(P0.05),牵张+冰敷组E_1与、牵张+冰敷组E_2存在显著性差异(P0.05)。实验结论:1、腓肠肌在发生损伤后,采用牵张、牵张+冰敷治疗,有利于损伤后骨骼肌的康复,具有促进愈合的效果。2、腓肠肌急性拉伤后采用牵张与牵张+冰敷治疗,腓肠肌拉伤修复过程中瘢痕组织的生成减少。3、腓肠肌修复过程中,牵张训练在后期治疗更有利于骨骼肌修复。
[Abstract]:Objective to investigate the repair process of gastrocnemius muscle after acute stretch injury by observing tissue sections at different time points after gastrocnemius injury. The repair process of tissue sections of gastrocnemius muscle at different time points after acute stretch injury after the intervention of stretch ice compress was studied, and the scientific treatment method of gastrocnemius muscle injury was determined. The effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle was observed by observing the effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle. To investigate the effect and mechanism of stretch and stretch ice compress on gastrocnemius muscle after acute injury. Methods: 64 male Sprague-Dawley rats were randomly divided into normal group A, immediate injury group B, natural recovery group C1, natural recovery group C 2, distraction group D 1, distraction group D 2, distraction ice compress group E _ 1. Stretch ice compress group Es 2 group. Gastrocnemius muscle injury model was established in 56 rats except normal group. After the establishment of the experimental model, D1 group and D2 group began to recover with distraction technique 12 days after the establishment of the model. The daily fixed time was fixed. After 12 days after the establishment of the experimental model, the two groups were rehabilitated by distraction technique after the establishment of the experimental model, and then ice compress was carried out. Daily fixed time, personnel operation. The rest group was fed normally. The samples of gastrocnemius muscle were taken at the corresponding time, and paraffin sections were made to observe and test the related indexes. The results of the experiment were as follows: 1. The results of the observation of gastrocnemius muscle light microscope in rats were as follows: after one hour of tensile injury, the tissue structure of muscle fibers in the damaged area was irregular, accompanied by edema, and the gap between muscle fibers was not consistent. Some obviously enlarged, the existence of inflammation can be observed, and vasodilation can be seen. On the 18th day after skeletal muscle injury, it was observed that the muscle fibers were repaired in the injured part of the natural recovery group, but not completely repaired. The size of the muscle fibers was different and the arrangement was not neat. After treatment, the new muscle fibers in the injured areas were more neatly arranged and more complete in structure after treatment in the distraction group and the stretch ice group. After 25 days of skeletal muscle injury, it can be seen that the muscle fibers in the natural recovery group were repaired, scar was repaired, the size of muscle fiber was different, and the arrangement was not neat. After treatment, the neonate muscle fibers in the injured areas were arranged neatly and repaired well. The results of immunohistochemical staining of collagen COL-I in gastrocnemius muscle of rats showed that the expression of COL-I protein decreased immediately after one hour of injury. On the 18th day after injury, the expression of collagen COL-I protein in the natural recovery group, D1 group and ice compress group showed the maximum expression. There was significant difference between group B1 and normal group (P0.01), and between group B and group B immediately after injury (P0.01). The expression of COL-I protein in group D1, group E1, group E1 and group B immediately after injury in distraction group was significantly different from that in group B (P0.01). There was a significant difference in the expression of COL-I protein in normal group (P0.05). There was no significant difference in COL-I protein expression between the two groups and the normal group on the 25th day after injury (P0.05). There was no significant difference in the expression of COL-I protein between the two groups (P 0.05), the distraction group (D2), the stretch ice compress group (E2 group) and the normal group (P 0.05). There was no significant difference in E _ 1 between D _ 1 and D _ 1 stretch ice compress group, but no significant difference in E _ (2) in D _ 2 stretch ice compress group. There was significant difference between D1 and D2 in distraction group (P0.05), significant difference in ESP 1 and E2 in distraction ice compress group (P0.05). Conclusion: 1. After injury, the gastrocnemius muscle was treated with stretch and stretch ice compress, which was beneficial to the recovery of skeletal muscle after injury, and had the effect of promoting healing. The gastrocnemius muscle was treated with stretch and stretch ice compress after acute injury of gastrocnemius. During the repair of gastrocnemius, the scar tissue formation decreased by 0.3. During the repair of gastrocnemius muscle, stretch training was more beneficial to skeletal muscle repair.
【学位授予单位】：河南师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R87
本文编号：2123730
[Abstract]:Objective to investigate the repair process of gastrocnemius muscle after acute stretch injury by observing tissue sections at different time points after gastrocnemius injury. The repair process of tissue sections of gastrocnemius muscle at different time points after acute stretch injury after the intervention of stretch ice compress was studied, and the scientific treatment method of gastrocnemius muscle injury was determined. The effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle was observed by observing the effect of stretch and stretch ice compress therapy on the expression of COL-I protein in injured skeletal muscle. To investigate the effect and mechanism of stretch and stretch ice compress on gastrocnemius muscle after acute injury. Methods: 64 male Sprague-Dawley rats were randomly divided into normal group A, immediate injury group B, natural recovery group C1, natural recovery group C 2, distraction group D 1, distraction group D 2, distraction ice compress group E _ 1. Stretch ice compress group Es 2 group. Gastrocnemius muscle injury model was established in 56 rats except normal group. After the establishment of the experimental model, D1 group and D2 group began to recover with distraction technique 12 days after the establishment of the model. The daily fixed time was fixed. After 12 days after the establishment of the experimental model, the two groups were rehabilitated by distraction technique after the establishment of the experimental model, and then ice compress was carried out. Daily fixed time, personnel operation. The rest group was fed normally. The samples of gastrocnemius muscle were taken at the corresponding time, and paraffin sections were made to observe and test the related indexes. The results of the experiment were as follows: 1. The results of the observation of gastrocnemius muscle light microscope in rats were as follows: after one hour of tensile injury, the tissue structure of muscle fibers in the damaged area was irregular, accompanied by edema, and the gap between muscle fibers was not consistent. Some obviously enlarged, the existence of inflammation can be observed, and vasodilation can be seen. On the 18th day after skeletal muscle injury, it was observed that the muscle fibers were repaired in the injured part of the natural recovery group, but not completely repaired. The size of the muscle fibers was different and the arrangement was not neat. After treatment, the new muscle fibers in the injured areas were more neatly arranged and more complete in structure after treatment in the distraction group and the stretch ice group. After 25 days of skeletal muscle injury, it can be seen that the muscle fibers in the natural recovery group were repaired, scar was repaired, the size of muscle fiber was different, and the arrangement was not neat. After treatment, the neonate muscle fibers in the injured areas were arranged neatly and repaired well. The results of immunohistochemical staining of collagen COL-I in gastrocnemius muscle of rats showed that the expression of COL-I protein decreased immediately after one hour of injury. On the 18th day after injury, the expression of collagen COL-I protein in the natural recovery group, D1 group and ice compress group showed the maximum expression. There was significant difference between group B1 and normal group (P0.01), and between group B and group B immediately after injury (P0.01). The expression of COL-I protein in group D1, group E1, group E1 and group B immediately after injury in distraction group was significantly different from that in group B (P0.01). There was a significant difference in the expression of COL-I protein in normal group (P0.05). There was no significant difference in COL-I protein expression between the two groups and the normal group on the 25th day after injury (P0.05). There was no significant difference in the expression of COL-I protein between the two groups (P 0.05), the distraction group (D2), the stretch ice compress group (E2 group) and the normal group (P 0.05). There was no significant difference in E _ 1 between D _ 1 and D _ 1 stretch ice compress group, but no significant difference in E _ (2) in D _ 2 stretch ice compress group. There was significant difference between D1 and D2 in distraction group (P0.05), significant difference in ESP 1 and E2 in distraction ice compress group (P0.05). Conclusion: 1. After injury, the gastrocnemius muscle was treated with stretch and stretch ice compress, which was beneficial to the recovery of skeletal muscle after injury, and had the effect of promoting healing. The gastrocnemius muscle was treated with stretch and stretch ice compress after acute injury of gastrocnemius. During the repair of gastrocnemius, the scar tissue formation decreased by 0.3. During the repair of gastrocnemius muscle, stretch training was more beneficial to skeletal muscle repair.
【学位授予单位】：河南师范大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R87
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