补充亮氨酸、葡萄糖对运动大鼠心肌自噬相关指标的影响

发布时间：2018-07-15 20:12

【摘要】：目的:为探讨运动员在葡萄糖和亮氨酸干预后心肌能量代谢的调控机制,拟建立运动大鼠模型阐述运动员心肌的结构和功能的变化机制。Beclin-1和LC-3II作为衡量自噬活性的关键蛋白可被m TOR负调控,而AMPK通过抑制m TOR来促进自噬,AMPK和m TOR协同作用共同调控心肌的能量代谢。方法:雄性SD大鼠80只,进行4周递增负荷跑台训练,适应性训练后,按随机对照原则将实验大鼠分为安静对照组(Con)、单纯补葡萄糖组(G)、单纯补亮氨酸组(L)、单纯补葡萄糖补亮氨酸组(G+L)、单纯运动组(E)、运动补葡萄糖组(E+G)、运动补亮氨酸组(E+L)和运动补葡萄糖补亮氨酸组(E+G+L)共计8组;在运动后进行生理盐水、葡萄糖、亮氨酸以及混合补剂的灌胃;western bolt检测心肌中LC3-II、Beclin-1、P-MTOR、MTOR、P-AMPK和AMPK的活性表达,实验数据采用spss22.0双因素方差分析进行统计处理。结果:(1)运动能够激活自噬蛋白Beclin-1和LC-3II的活性,且运动后24h时p-m TOR/m TOR和p-AMPK/AMPK的比值恢复到安静水平;(2)补充葡萄糖和补充亮氨酸没能激活自噬蛋白Beclin-1和LC-3II的活性;p-m TOR/m TOR的比值没有变化;p-AMPK/AMPK的比值没有变化。结论:(1)适量强度的运动训练可增加自噬蛋白Beclin-1和LC3-II的表达;(2)补充适量的葡萄糖、亮氨酸对运动大鼠心肌自噬指标的表达无显著性差异。
[Abstract]:Objective: to investigate the regulation mechanism of myocardial energy metabolism in athletes after glucose and leucine intervention. In order to establish a rat model of exercise, the changes of myocardial structure and function of athletes. Beclin-1 and LC-3II as key proteins to measure autophagy activity can be negatively regulated by m TOR. AMPK promotes the synergistic effect of autophagy AMPK and mTOR to regulate myocardial energy metabolism by inhibiting mTOR. Methods: 80 male Sprague-Dawley rats were trained with incremental load for 4 weeks. According to the principle of random control, the experimental rats were divided into quiet control group (Con), glucose supplement group (G), leucine supplement group (L), glucose supplement group (G L), exercise group (E), exercise supplement glucose group (E G), exercise supplement leucine group (L). Group E (E L) and group E G L (E G L) were treated with exercise supplementation of glucose and leucine (E G L). After exercise, physiological saline, glucose, leucine and mixed supplement western bolt were used to detect the expression of P-AMPK and AMPK in myocardium. The experimental data were analyzed by spss22.0 double-factor ANOVA. Results: (1) exercise could activate the activities of autophagy proteins Beclin-1 and LC-3II. At 24 hours after exercise, the ratio of p-m torque / p-AMPK to p-AMPK returned to quiet level, (2) the ratio of p-m TOR-mTOR to p-AMPK did not change after 24 hours of exercise. (2) the ratio of p-AMPK / AMPK did not change when glucose and leucine supplementation did not activate the activity of autophagy protein Beclin-1 and LC-3II. Conclusion: (1) moderate intensity exercise training can increase the expression of Beclin-1 and LC3-II, and (2) there is no significant difference in the expression of myocardial autophagy index between rats supplemented with appropriate glucose and leucine.
【学位授予单位】：首都体育学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R87

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