加味酸枣仁汤对运动性失眠大鼠单胺类神经递质影响的研究
发布时间：2018-07-20 10:37
【摘要】：1.研究目的 通过观察运动性失眠大鼠服用复方中药后其整体特征及脑组织单胺类神经递质的动态变化，探讨复方中药对单胺类神经递质代谢的影响，更好的认识单胺类神经递质在睡眠-觉醒节律调节中的作用以及运动性失眠产生的机制，为有效解决运动性失眠和指导运动训练提供科学依据。 2.研究方法 选取8周龄健康雌性wistar大鼠60只，随机分为对照组、运动性失眠模型组、自然康复组、加味酸枣仁汤低剂量组、加味酸枣仁汤高剂量组、安定片组，每组10只。6周相关干预后各组大鼠断头处死，开颅取脑。采用高效液相色谱仪-荧光检测器检测大鼠脑组织内5-羟色胺、5-羟吲哚乙酸、多巴胺、去甲肾上腺素的含量。所有数据采用SPSS17.0统计软件进行统计学处理。 3.实验结果 3.1与对照组比较，模型组大鼠脑组织内单胺类递质5-HT及其代谢产物5-HIAA含量均极明显降低（p0.001），NE和DA的含量及DA/5-HT的值则明显升高（p0.01或p0.001）。 3.2与模型组比较，加味酸枣仁汤高剂量组大鼠脑组织内5-HT及其代谢产物5-HIAA含量均极明显升高（p0.001），NE和DA的含量及DA/5-HT的值则极明显降低（p0.001）。 3.3与西药组比较，加味酸枣仁汤高剂量组大鼠脑组织内5-HT及其代谢产物5-HIAA含量明显升高（p0.05），，NE含量及DA/5-HT的值则明显降低（p0.05）。 3.4与对照组比较，在建模后期，其他各组实验大鼠体重与摄食量均显著性降低（p0.001），经加味酸枣仁汤治疗后，虽都有所增加，但还是非常明显低于对照组（p0.01）。 4.结论 4.1采用大负荷游泳训练+腹腔注射PCPA所致的失眠大鼠模型，符合中医学虚劳失眠症候模型，表明采用该方法建立动物运动失眠模型具有较强的可行性。 4.2运动性失眠可导致大鼠体重和摄食量的减少，加味酸枣仁汤能促进大鼠运动疲劳的恢复和改善大鼠睡眠和摄食质量。 4.3运动性失眠可以导致大鼠脑组织内单胺类神经递质5-HT及其代谢产物5-HIAA含量明显降低，而NE、DA及DA/5-HT的值明显增加。加味酸枣仁汤干预可提高脑组织5-HT和5-HIAA含量，降低NE、DA及DA/5-HT的值，改善睡眠，保护大脑。而且加味酸枣仁汤的疗效与量效关系密切。
[Abstract]:1. Objective to investigate the effects of compound Chinese medicine on the metabolism of monoamine neurotransmitters by observing the overall characteristics of rats with exercise insomnia and the dynamic changes of monoamine neurotransmitters in brain tissue. To better understand the role of monoamine neurotransmitters in the regulation of sleep-arousal rhythm and the mechanism of motor insomnia. 2. Methods Sixty healthy female wistar rats aged 8 weeks were randomly divided into three groups: control group, model group of exercise insomnia, natural rehabilitation group, low dose group of modified Suanzaoren decoction, high dose group of modified Suanzaoren decoction and diazepam tablet group. 10 rats in each group were killed after 6. 6 weeks of intervention and craniotomy. The contents of 5-hydroxyindoleacetic acid, dopamine and norepinephrine in rat brain were determined by high performance liquid chromatograph and fluorescence detector. All the data were processed by SPSS 17.0 statistical software. Results 3.1 compared with the control group, the contents of monoamine transmitter 5-HT and its metabolite 5-HIAA in the brain of the model group were significantly decreased (p0.001), the contents of NE and DA, and the value of DA-5-HT were significantly increased (p0.01 or p0.001). 3.2 compared with the model group, the contents of 5-HT and 5-HIAA in the brain tissue of the model group were significantly higher than those in the model group. The contents of 5-HT and its metabolite 5-HIAA in brain tissue of rats with high dosage of Jiawei Suanzaoren decoction were significantly increased (p0.001), the contents of NE and DA, and the value of DA-5-HT were significantly decreased (p0.001). 3.3 compared with western medicine group, the content of 5-HT and 5-HIAA in brain tissue of rats were significantly lower than those in western medicine group. The contents of 5-HT and its metabolite 5-HIAA in brain tissue of rats with high dosage of Jiawei Suanzaoren decoction were significantly increased (p0.05) and the value of DA-5-HT were significantly decreased (p0.05). 3.4 compared with the control group, the content of 5-HT and its metabolite 5-HIAA were significantly decreased in the later stage of modeling. The body weight and food intake of other experimental rats were significantly decreased (p0.001). After treated with modified Suanzaoren decoction, the rats were all increased, but still significantly lower than the control group (p0.01). Conclusion 4.1 Insomnia rat model induced by intraperitoneal injection of PCPA with heavy load swimming training is in line with the model of asthenia insomnia in traditional Chinese medicine. The results showed that it was feasible to establish the animal model of exercise insomnia by using this method. 4.2 exercise insomnia could reduce the body weight and food intake of rats. Jiawei Suanzaoren decoction could promote the recovery of exercise fatigue and improve the sleep and feeding quality of rats. 4.3 the content of monoamine neurotransmitter 5-HT and its metabolite 5-HIAA in rat brain was significantly decreased after exercise insomnia. However, the values of DA and DA / D / 5 HT increased significantly. The intervention of Jiawei Suanzaoren decoction could increase the contents of 5-HT and 5-HIAA in brain tissue, decrease the values of NEDA and DA-5-HT, improve sleep and protect the brain. Moreover, the curative effect of modified Suanzaoren decoction is closely related to its dose effect.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285.5;R87
本文编号：2133256
[Abstract]:1. Objective to investigate the effects of compound Chinese medicine on the metabolism of monoamine neurotransmitters by observing the overall characteristics of rats with exercise insomnia and the dynamic changes of monoamine neurotransmitters in brain tissue. To better understand the role of monoamine neurotransmitters in the regulation of sleep-arousal rhythm and the mechanism of motor insomnia. 2. Methods Sixty healthy female wistar rats aged 8 weeks were randomly divided into three groups: control group, model group of exercise insomnia, natural rehabilitation group, low dose group of modified Suanzaoren decoction, high dose group of modified Suanzaoren decoction and diazepam tablet group. 10 rats in each group were killed after 6. 6 weeks of intervention and craniotomy. The contents of 5-hydroxyindoleacetic acid, dopamine and norepinephrine in rat brain were determined by high performance liquid chromatograph and fluorescence detector. All the data were processed by SPSS 17.0 statistical software. Results 3.1 compared with the control group, the contents of monoamine transmitter 5-HT and its metabolite 5-HIAA in the brain of the model group were significantly decreased (p0.001), the contents of NE and DA, and the value of DA-5-HT were significantly increased (p0.01 or p0.001). 3.2 compared with the model group, the contents of 5-HT and 5-HIAA in the brain tissue of the model group were significantly higher than those in the model group. The contents of 5-HT and its metabolite 5-HIAA in brain tissue of rats with high dosage of Jiawei Suanzaoren decoction were significantly increased (p0.001), the contents of NE and DA, and the value of DA-5-HT were significantly decreased (p0.001). 3.3 compared with western medicine group, the content of 5-HT and 5-HIAA in brain tissue of rats were significantly lower than those in western medicine group. The contents of 5-HT and its metabolite 5-HIAA in brain tissue of rats with high dosage of Jiawei Suanzaoren decoction were significantly increased (p0.05) and the value of DA-5-HT were significantly decreased (p0.05). 3.4 compared with the control group, the content of 5-HT and its metabolite 5-HIAA were significantly decreased in the later stage of modeling. The body weight and food intake of other experimental rats were significantly decreased (p0.001). After treated with modified Suanzaoren decoction, the rats were all increased, but still significantly lower than the control group (p0.01). Conclusion 4.1 Insomnia rat model induced by intraperitoneal injection of PCPA with heavy load swimming training is in line with the model of asthenia insomnia in traditional Chinese medicine. The results showed that it was feasible to establish the animal model of exercise insomnia by using this method. 4.2 exercise insomnia could reduce the body weight and food intake of rats. Jiawei Suanzaoren decoction could promote the recovery of exercise fatigue and improve the sleep and feeding quality of rats. 4.3 the content of monoamine neurotransmitter 5-HT and its metabolite 5-HIAA in rat brain was significantly decreased after exercise insomnia. However, the values of DA and DA / D / 5 HT increased significantly. The intervention of Jiawei Suanzaoren decoction could increase the contents of 5-HT and 5-HIAA in brain tissue, decrease the values of NEDA and DA-5-HT, improve sleep and protect the brain. Moreover, the curative effect of modified Suanzaoren decoction is closely related to its dose effect.
【学位授予单位】：曲阜师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R285.5;R87
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