运动对TCDD染毒大鼠肝脏Nrf2及相关蛋白表达的影响

发布时间：2018-08-04 17:49

【摘要】：目的:以SD大鼠作为实验对象,研究运动和二恶英染毒对SD大鼠肝组织Nrf2及相关蛋白表达的影响。方法:将40只7周龄雄性大鼠适应性喂养一周后随机分为静养对照组(NC),运动对照组(EC),染毒静养组(NT),染毒运动组(ET)。NT,ET腹腔注射6.4μg/kg体重的TCDD;NC,EC大鼠注射等量的玉米油,每隔一周给予上述剂量的21%TCDD染毒维持剂量,持续染毒共8周。EC,ET进行8周的运动,运动方案为大鼠尾部负重5%体重,进行游泳运动,每次运动30分钟,每周运动5天。实验结束取材,检测大鼠体重,肝脏重量,肝脏相对重量,检测大鼠血清AST、ALT的活性,检测Keap1、Nrf2蛋白含量,检测Nrf2-ARE结合活性。结果:染毒对大鼠肝湿重的影响有主效应(P=0.049);染毒对大鼠肝湿重/体重的影响有主效应(P=0.049);运动对大鼠血清AST活性的影响有主效应(P=0.020),染毒对大鼠血清AST活性的影响有主效应(P=0.001);运动对血清ALT活性的影响有主效应(P=0.001),染毒对血清ALT活性的影响有主效应(P=0.049)。NC组和EC组大鼠肝细胞排列整齐,细胞结构完整,NT组大鼠细胞排列紊乱,伴有炎症细胞浸润,NE组大鼠细胞排列稍显紊乱,伴有炎症细胞浸润;运动对肝脏Nrf2总蛋白的表达有主效应(P=0.004),染毒对肝脏Nrf2总蛋白的表达有主效应(P=0.004);运动对肝脏Nrf2核蛋白的表达主效应(P=0.000),染毒对肝脏Nrf2核蛋白的表达有可靠的主效应(P=0.015),运动和染毒对肝脏Nrf2核蛋白的表达有交互效应(P=0.024);运动对肝脏细胞核Nrf2-ARE结合活性有主效应(P0.001);染毒对肝脏细胞核Nrf2-ARE结合活性有主效应(P0.001);运动和染毒对肝脏细胞核Nrf2-ARE结合活性有可靠的交互效应(P=0.043)结论:1.TCDD染毒使大鼠肝脏Nrf2核蛋白含量降低,抑制机体氧化应激水平;2.运动可以缓解TCDD诱导的肝脏Nrf2核蛋白含量降低,增强机体氧化应激能力;3.运动可以通过上调Nrf2及其相关蛋白的表达来有效降低TCDD导致的肝脏损伤。
[Abstract]:Aim: to study the effects of exercise and dioxin exposure on the expression of Nrf2 and related proteins in the liver of SD rats. Methods: forty 7-week-old male rats were randomly divided into control group (NC), exercise control group (EC), (NT), control group (ET). NTX et) after one week of adaptive feeding. The rats were injected with the same amount of corn oil by intraperitoneal injection of 6.4 渭 g/kg weight TCDDNCCEC rats. Every other week, the above dose of 21%TCDD was administered to maintain dose, and continuous exposure lasted for 8 weeks. The exercise program was 5% body weight bearing on the tail of the rats, swimming for 30 minutes each time, 5 days a week. At the end of the experiment, the body weight, liver weight and relative weight of liver were measured. The activity of alt, the protein content of Keap1Nrf2 and the binding activity of Nrf2-ARE were detected. Results: there were main effects on liver wet weight (P0.049), main effect on wet weight / body weight of rat liver (P0. 049), main effect on serum AST activity of rats (P0. 020) and effect on serum AST activity of rats after exposure to DW (P0. 020), P0. 049 (P0. 049), P0. 020 (P0. 020) and P0. 020 (P0. 020) respectively. The effect of exercise on serum ALT activity was significant (P0. 001), and the effect of exposure on serum ALT activity was significant (P0. 049). The hepatocytes of NC group and EC group were arranged neatly. In the NT group, the cell arrangement was disordered, and the inflammatory cell infiltration was found in the NE group. The cells in the NE group were slightly disordered, and the inflammatory cell infiltration was also found in the NE group. Exercise has a major effect on the expression of total Nrf2 protein in liver (P0. 004), a major effect on the expression of total protein of Nrf2 in liver (P0. 004), a major effect of exercise on the expression of Nrf2 nucleoprotein in liver (P0. 000), a reliable major effect on the expression of Nrf2 nuclear protein in liver (P0. 015). Exercise and exposure had an interactive effect on the expression of Nrf2 nucleoprotein in liver (P0. 024); exercise had a major effect on nuclear Nrf2-ARE binding activity of liver (P0. 001); exposure to exercise had a major effect on Nrf2-ARE binding activity in liver nucleus (P0. 001); exercise and exposure had a major effect on Nrf2-ARE binding activity in liver nucleus (P0. 001); exercise and exposure had a major effect on Nrf2-ARE binding activity in liver nucleus (P0. 001). Conclusion: 1. TCDD can reduce the content of Nrf2 nuclear protein in rat liver. Inhibition of oxidative stress level 2. Exercise can alleviate the decrease of Nrf2 nuclear protein content in liver induced by TCDD, and enhance the oxidative stress ability of the body. Exercise can effectively reduce liver injury induced by TCDD by up-regulating the expression of Nrf2 and its associated proteins.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R87

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