正加速度适应性训练对大鼠胃黏膜的保护作用及机制研究
发布时间：2018-08-14 16:42
【摘要】：目的通过建立大鼠正加速度(positive acceleration,+Gz)适应性训练模型,观察适应性训练对各组大鼠接受正加速度处理后胃黏膜损伤程度影响,检测大鼠胃黏膜内前列环素(prostacyclin,PGI_2)、血栓素A2(thromboxane A2,TXA2)、TXA2/PGI_2比值、前列腺素E2(Prostaglandin E2,PGE_2)、环氧合酶-1 mRNA(COX-1 mRNA)和环氧合酶-2 mRNA(COX-2 mRNA)含量的变化,探讨正加速度适应性训练对大鼠胃黏膜保护作用的相关机制,从而为飞行员及航天员胃黏膜损伤相关疾病的防止提供理论依据。方法40只雄性SD大鼠随机分为5组,每组8只,分别标记为为A、B、C、D、E组。A组大鼠为空白对照,不做处理,B组大鼠+5Gz值暴露5min,每天一次,连续暴露5天,C组大鼠+10Gz值暴露5min,每天一次,连续暴露5天,D组大鼠适应性训练(即+4Gz值暴露3min,每天1次,连续暴露5天)后+5Gz值暴露5min,每天1次,连续暴露5天,E组大鼠适应性训练(即+4Gz值暴露3min,每天1次,连续暴露5天)后+10Gz值暴露5min,每天1次,连续暴露5天。试验结束后肉眼和光学显微镜下观察胃黏膜损伤情况,计算损伤指数,ELISA法检测胃黏膜内血栓素B_2(thromboxane B_2,TXB_2)、6-酮-前列腺素F1α(6-Keto-Prostaglandin F1α,6-Keto-PGF1α)、前列腺素E2(Prostaglandin E2,PGE_2)的含量,计算TXB_2/6-K-PGF1a的比值,RT-PCR法检测胃黏膜内COX-1 mRNA和COX-2 mRNA的相对表达量。结果(1)肉眼观察,除A组外,其余各组胃黏膜均可见不同程度损伤。C组损伤指数高于B组(5.625±1.767 vs 1.750±0.707,P0.05)。适应性训练后,D组损伤指数低于B组(0.875±0.641 vs 1.750±0.707,P0.05),E组损伤指数低于C组(2.250±1.035 vs 5.625±1.767,P0.05)。(2)B组TXB_2含量高于A组(251.018 pg/ml±50.845 pg/ml vs 121.400pg/ml±41.629 pg/ml,P0.05),C组TXB_2含量高于B组(331.538 pg/ml±79.102pg/ml vs 251.018 pg/ml±50.845 pg/ml,P0.05),适应性训练后,D组TXB_2含量低于B组(159.588 pg/ml±36.216 pg/ml vs 251.018 pg/ml±50.845 pg/ml,P0.05),E组TXB_2含量低于C组(150.476 pg/ml±48.589 pg/ml vs 331.538 pg/ml±79.102pg/ml,P0.05)。B组6-Keto-PGF1a低于A组(52.015 pg/ml±11.827 pg/ml vs 106.322 pg/ml±16.909pg/ml,P0.05),C组6-Keto-PGF1a低于A组(44.726 pg/ml±18.867 pg/ml vs106.322 pg/ml±16.909 pg/ml,P0.05)。适应性训练后,D组6-Keto-PGF1a高于B组(72.242 pg/ml±12.413 pg/ml vs 52.015 pg/ml±11.827 pg/ml,P0.05),E组6-Keto-PGF1a高于C组(87.426 pg/ml±15.833 pg/ml vs 44.726 pg/ml±18.867 pg/ml,P0.05)。B组TXB_2/6-K-PGF1a比值大于A组(5.128±1.788 vs 1.199±0.545,P0.05),C组TXB_2/6-K-PGF1a比值大于B组(8.599±4.157 vs 5.128±1.788,P0.05),适应性训练后,D组TXB_2/6-K-PGF1a比值小于B组(2.283±0.705 vs 5.128±1.788,P0.05),E组TXB_2/6-K-PGF1a比值小于C组(2.250±1.035 vs 8.599±4.157,P0.05)。(3)B组PGE_2含量低于A组(60.468 pg/ml±9.697 pg/ml vs 81.462 pg/ml±20.340pg/ml,P0.05),C组PGE_2含量低于B组(24.598 pg/ml±6.017 pg/ml vs 60.468pg/ml±9.697 pg/ml,P0.05),适应性训练后,E组PGE_2含量高于C组(46.165pg/ml±13.996 vs 24.598±6.017 pg/ml,P0.05)(4)COX-1 mRNA相对表达量各组间差异无统计学意义(P0.05)。B组COX-2mRNA表达高于A组(1.492±0.281 vs 0.978±0.109,P0.05),C组COX-2 mRNA表达高于A组和B组(2.642±0.320 vs 0.978±0.109,P0.05;2.642±0.320 vs1.492±0.281,P0.05)。适应性训练后,D组COX-2 mRNA表达高于B组(1.782±0.099 vs 1.492±0.281,P0.05),E组COX-2 mRNA表达高于C组(3.268±0.083 vs 2.642±0.320,P0.05)。(5)各组6-Keto-PGF1α含量与COX-1 mRNA和COX-2 mRNA相对表达量间均无相关性,各组PGE_2含量与COX-1 mRNA和COX-2 mRNA相对表达量间均无相关性。结论正加速度适应性训练可减轻高强度正加速度带来的胃黏膜损伤,其机制与PGI_2、PGE_2含量升高、TXA2含量降低、TXA2/PGI_2比值降低及COX-2mRNA表达增加有关。
[Abstract]:Objective To observe the effects of positive acceleration (+Gz) on gastric mucosal injury after positive acceleration in rats, and to detect the prostacyclin (PGI_2), thromboxane A2 (TXA2), TXA2/PGI_2 ratio and prostaglandin E2 (P Rostaglandin E2, PGE_2, cyclooxygenase-1 mRNA (COX-1 mRNA) and cyclooxygenase-2 mRNA (COX-2 mRNA) levels were measured to investigate the protective effects of positive acceleration adaptive training on gastric mucosa in rats, so as to provide a theoretical basis for the prevention of gastric mucosal injury-related diseases in pilots and astronauts. Methods Forty male SD rats were randomly divided into two groups. Rats in group A were exposed to +5Gz for 5 minutes once a day for 5 days. Rats in group C were exposed to +10Gz for 5 minutes once a day for 5 consecutive days, once a day for 5 consecutive days. Rats in group D were exposed to +5Gz for 3 minutes, once a day for 5 consecutive days. Group E rats were exposed to + 10 Gz for 5 minutes, once a day for 5 days after adaptive training (i.e. +4 Gz exposure for 3 minutes, once a day for 5 consecutive days). The contents of E B_2, TXB_2, 6-keto-prostaglandin F1a (6-Keto-Prostaglandin F1a, 6-Keto-PGF_1a), prostaglandin E2 (PGE_2), the ratio of TXB_2/6-K-PGF_1a, and the relative expression of COX-1 mRNA and COX-2 mRNA in gastric mucosa were measured by RT-PCR. Results (1) The gastric mucosa of all groups except group A was observed with naked eyes. The injury index of group C was higher than that of group B (5.625 + 1.765 vs 1.767 vs 1.750 vs 1.750 vs 1.750 vs 0.707, P 0.05). After adaptive training, the injury index of group D was lower than that of group B (0.875 + 0.641 vs 1.750 vs 1.750 vs 1.700 vs 1.707, P 0.05) after adaptive training, the injury index of group E was lower than that of group C (2.250 + 1.250 + 1.035 vs 5.035.625 vs 5.625 5 5 5.625 + 1.767, P 0.05). (2) TXB_2 content in group B was higher than that in group A (251.018.018 pg/629 The TXB_2 content in group C was higher than that in group B (331.538 pg/ml (+79.102pg/ml (+79.102pg/ml (+ 79.102 pg/ml vs 251.018 pg/ml vs 251.018 pg/ml (+50.845 pg/ml, P 0.05)). After adaptive training, the TXB_2 content in group D was lower than that in group B (159.588 8 8 8 8 8 pg/ml (+36.216pg/ml (+36.216pg/ml +36.216pg/ml) vs 251.018 pg/ml (+50.845.845 pg/ml, P 0.05), TXB_2 content in group vs 331.538 pg/ml (+ 79.102 pg/ml), The 6-Keto-PGF1a in group B was lower than that in group A (52.015 pg/ml (11.827 pg / ml (16.909 pg / ml, P 0.05), 6-Keto-PGF1a in group C was lower than that in group A (44.726 pg / ml ml vs 52.015 pg/ml+11.827 pg/ml, P 0.05, 6-Keto-PGF1a in E group was higher than that in C group (87.426 pg/ml (+15.833 pg/ml (+15.833 pg/ml vs 44.726 pg/ml (+18.867 pg/ml (+18.867 pg/ml, P 0.05). TXB_2/6-K-PGF1a ratio in B group was higher than that in A group (5.128 (+1.128 [1.788 vs 1.788 vs 1.788 vs 1.199 [1.199 [0.545,P 0.05, P 0.05). TXB_2/6-K-PGF1a ratio in C group was higher than that in B group (8.599 [(8.599 The ratio of TXB_2/6-K-PGF1a in group D was lower than that in group B (2.283+0). The ratio of TXB_2/6-K-PGF1a in E group was lower than that in C group (2.250 (+ 1.035 vs 8.599 (+ 4.157, P 0.05). (3) The content of PGE_2 in B group was lower than that in A group (60.468 pg/ml (+ 9.697 PG vs vs 9.697 pg/ml (+ 9.697 pg/ml vs vs vs 81.462 pg/ml [81.462 pg/ml [20.340 pg/ml [20.340 pg/ml, P 0.05). (3) The content of PGE_2 in E_2 in E_2 was lower than that in C group (24.598 P8 pg/ml (+9.697 pg/ml, P 0.05). After adaptive training, E. The content of PGE_2 in group B was higher than that in group A (46.165 pg/ml (+13.996 vs 24.598 +6.017 pg/ml, P 0.05) (4) There was no significant difference in the relative expression of COX-1 mRNA between groups (P 0.05). The expression of COX-2 mRNA in group B was higher than that in group A (1.492 +0.281 vs 0.978 +0.109, P 0.05). After adaptive training, the expression of COX-2 mRNA in group D was higher than that in group B (1.782.099 vs 1.492.0.281, P 0.05). The expression of COX-2 mRNA in group E was higher than that in group C (3.268.083 vs 2.642.320, P 0.05). Conclusion Positive acceleration adaptive training can alleviate gastric mucosal injury induced by high intensity positive acceleration. The mechanism is related to the increase of PGI_2 and PGE_2 contents, the decrease of TXA2 content, the decrease of TXA2/PGI_2 ratio and the increase of COX-2 mRNA expression.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R856.5
，
本文编号：2183480
[Abstract]:Objective To observe the effects of positive acceleration (+Gz) on gastric mucosal injury after positive acceleration in rats, and to detect the prostacyclin (PGI_2), thromboxane A2 (TXA2), TXA2/PGI_2 ratio and prostaglandin E2 (P Rostaglandin E2, PGE_2, cyclooxygenase-1 mRNA (COX-1 mRNA) and cyclooxygenase-2 mRNA (COX-2 mRNA) levels were measured to investigate the protective effects of positive acceleration adaptive training on gastric mucosa in rats, so as to provide a theoretical basis for the prevention of gastric mucosal injury-related diseases in pilots and astronauts. Methods Forty male SD rats were randomly divided into two groups. Rats in group A were exposed to +5Gz for 5 minutes once a day for 5 days. Rats in group C were exposed to +10Gz for 5 minutes once a day for 5 consecutive days, once a day for 5 consecutive days. Rats in group D were exposed to +5Gz for 3 minutes, once a day for 5 consecutive days. Group E rats were exposed to + 10 Gz for 5 minutes, once a day for 5 days after adaptive training (i.e. +4 Gz exposure for 3 minutes, once a day for 5 consecutive days). The contents of E B_2, TXB_2, 6-keto-prostaglandin F1a (6-Keto-Prostaglandin F1a, 6-Keto-PGF_1a), prostaglandin E2 (PGE_2), the ratio of TXB_2/6-K-PGF_1a, and the relative expression of COX-1 mRNA and COX-2 mRNA in gastric mucosa were measured by RT-PCR. Results (1) The gastric mucosa of all groups except group A was observed with naked eyes. The injury index of group C was higher than that of group B (5.625 + 1.765 vs 1.767 vs 1.750 vs 1.750 vs 1.750 vs 0.707, P 0.05). After adaptive training, the injury index of group D was lower than that of group B (0.875 + 0.641 vs 1.750 vs 1.750 vs 1.700 vs 1.707, P 0.05) after adaptive training, the injury index of group E was lower than that of group C (2.250 + 1.250 + 1.035 vs 5.035.625 vs 5.625 5 5 5.625 + 1.767, P 0.05). (2) TXB_2 content in group B was higher than that in group A (251.018.018 pg/629 The TXB_2 content in group C was higher than that in group B (331.538 pg/ml (+79.102pg/ml (+79.102pg/ml (+ 79.102 pg/ml vs 251.018 pg/ml vs 251.018 pg/ml (+50.845 pg/ml, P 0.05)). After adaptive training, the TXB_2 content in group D was lower than that in group B (159.588 8 8 8 8 8 pg/ml (+36.216pg/ml (+36.216pg/ml +36.216pg/ml) vs 251.018 pg/ml (+50.845.845 pg/ml, P 0.05), TXB_2 content in group vs 331.538 pg/ml (+ 79.102 pg/ml), The 6-Keto-PGF1a in group B was lower than that in group A (52.015 pg/ml (11.827 pg / ml (16.909 pg / ml, P 0.05), 6-Keto-PGF1a in group C was lower than that in group A (44.726 pg / ml ml vs 52.015 pg/ml+11.827 pg/ml, P 0.05, 6-Keto-PGF1a in E group was higher than that in C group (87.426 pg/ml (+15.833 pg/ml (+15.833 pg/ml vs 44.726 pg/ml (+18.867 pg/ml (+18.867 pg/ml, P 0.05). TXB_2/6-K-PGF1a ratio in B group was higher than that in A group (5.128 (+1.128 [1.788 vs 1.788 vs 1.788 vs 1.199 [1.199 [0.545,P 0.05, P 0.05). TXB_2/6-K-PGF1a ratio in C group was higher than that in B group (8.599 [(8.599 The ratio of TXB_2/6-K-PGF1a in group D was lower than that in group B (2.283+0). The ratio of TXB_2/6-K-PGF1a in E group was lower than that in C group (2.250 (+ 1.035 vs 8.599 (+ 4.157, P 0.05). (3) The content of PGE_2 in B group was lower than that in A group (60.468 pg/ml (+ 9.697 PG vs vs 9.697 pg/ml (+ 9.697 pg/ml vs vs vs 81.462 pg/ml [81.462 pg/ml [20.340 pg/ml [20.340 pg/ml, P 0.05). (3) The content of PGE_2 in E_2 in E_2 was lower than that in C group (24.598 P8 pg/ml (+9.697 pg/ml, P 0.05). After adaptive training, E. The content of PGE_2 in group B was higher than that in group A (46.165 pg/ml (+13.996 vs 24.598 +6.017 pg/ml, P 0.05) (4) There was no significant difference in the relative expression of COX-1 mRNA between groups (P 0.05). The expression of COX-2 mRNA in group B was higher than that in group A (1.492 +0.281 vs 0.978 +0.109, P 0.05). After adaptive training, the expression of COX-2 mRNA in group D was higher than that in group B (1.782.099 vs 1.492.0.281, P 0.05). The expression of COX-2 mRNA in group E was higher than that in group C (3.268.083 vs 2.642.320, P 0.05). Conclusion Positive acceleration adaptive training can alleviate gastric mucosal injury induced by high intensity positive acceleration. The mechanism is related to the increase of PGI_2 and PGE_2 contents, the decrease of TXA2 content, the decrease of TXA2/PGI_2 ratio and the increase of COX-2 mRNA expression.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R856.5
，
本文编号：2183480
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