有氧运动预防大鼠2型糖尿病过程中对机体铁代谢的影响

发布时间：2018-08-18 15:39

【摘要】：目的:探讨有氧运动预防高糖高脂饮食诱导大鼠2型糖尿病过程中,通过缓解机体铁超载,减轻氧化应激,改善胰岛素抵抗的机制。方法:24只12月龄SD大鼠,随机分为对照组(C组)、高糖高脂膳食组(H组)、高糖高脂膳食结合有氧运动组(HE组),每组8只。C组进行基础饲料喂养,其余两组使用高糖高脂饲料。HE组在2周适应运动后,进行15 m/min、45 min/天、坡度5度、6天/周、3周有氧运动干预,其余两组安静喂养。5周后,用酶联免疫吸附实验(ELISA)检测血清铁蛋白、血清胰岛素;生化试剂盒检测血清铁、血清总铁结合力、肝组织铁、血糖、肝超氧化物歧化酶(SOD)、肝丙二醛(MDA)、肝过氧化氢酶(CAT);肝组织切片用苏木精-伊红(HE)染色。结果:(1)HE组血清铁蛋白、血清胰岛素、血糖、胰岛素抵抗指数显著高于C组,显著低于H组(P0.05,P0.01)。HE组肝SOD、肝CAT、胰岛素敏感指数显著高于H组(P0.05,P0.01),H组显著低于C组(P0.05,P0.01);(2)HE组肝MDA、肝组织铁、血清铁、转铁蛋白饱和度显著低于H组(P0.01)。(3)组织学观察表明,HE组较H组肝细胞脂肪滴数量少,无小灶状炎症细胞浸润。结论:(1)长期高糖高脂饮食诱导大鼠体重增加,加重铁过载和氧化应激,出现胰岛素抵抗。(2)有氧运动能够降低机体铁储存,增加抗氧化应激酶的活性,缓解氧化应激与胰岛素抵抗,减轻高脂膳食大鼠肝组织脂肪样变。
[Abstract]:Objective: to investigate the mechanism of aerobic exercise in preventing type 2 diabetes induced by high glucose and high fat diet in rats by relieving iron overload, reducing oxidative stress and improving insulin resistance. Methods Twenty four 12-month-old SD rats were randomly divided into three groups: control group (group C), high-sugar high-fat diet group (H group), high-sugar high-fat diet combined with aerobic exercise group (HE group). The other two groups were treated with high sugar and high fat diet. After 2 weeks of adaptation to exercise, the other two groups were treated with aerobic exercise for 15 m / min for 45 min/ and 5 degrees / week for 6 days / week for 3 weeks. The rest two groups were fed quietly for 5 weeks. The serum ferritin levels were detected by enzyme linked immunosorbent assay (ELISA). Serum insulin, serum total iron binding capacity, liver iron, blood glucose, liver superoxide dismutase (SOD), malondialdehyde (MDA),) liver catalase (CAT);), liver tissue sections were stained with hematoxylin and eosin (HE). Results: (1) the levels of serum ferritin, serum insulin, blood glucose, insulin resistance index in HE group were significantly higher than those in C group, and significantly lower than those in H group (P0.05, P0.01). Serum iron and transferrin saturation were significantly lower than those in H group (P0.01). (3). The results showed that the number of fat droplets in liver cells in the HE group was less than that in the H group, and there was no small inflammatory cell infiltration. Conclusion: (1) Long-term high glucose and high fat diet could induce weight gain, increase iron overload and oxidative stress, and induce insulin resistance in rats. (2) aerobic exercise could decrease iron storage and increase the activity of antioxidant kinase. Alleviate oxidative stress and insulin resistance, and alleviate fatty changes in liver tissue of rats with high fat diet.
【作者单位】：河北师范大学体育学院人体运动生物信息测评重点实验室;石家庄市第一医院;
【基金】：河北省自然科学基金(C2013205026) 河北省教育厅重点基金(ZD20131100) 河北师范大学研究生基金(201302023) 河北省体育教育训练学重点学科资助河北师范大学体育学重点学科
【分类号】：R87

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