不可逆电穿孔与冷冻治疗消融猪肝脏组织对比分析

发布时间：2018-08-30 12:10

【摘要】：目的对比分析不可逆电穿孔(IRE)及冷冻治疗对猪肝实质的消融效果。方法将4只实验用小型猪,随机分为2组,每组2只。第1组先于CT引导下采用IRE及冷冻治疗分别进行肝脏消融,消融后常规饲养;30天后以同法消融第2组小型猪,术中及术后对小型猪行螺旋CT平扫及增强扫描,扫描完成后处死两组实验动物并取出肝脏,采用10%甲醛溶液固定,将标本整体切片行病理学检查。结果术中CT平扫显示冷冻消融区域边界清晰,IRE消融边界不清;术后增强CT扫描显示IRE及冷冻消融区域术后即刻均未见强化。大体标本:IRE消融区域边界清晰,血管、胆管结构良好;冷冻消融区域边界模糊,局部可见出血。结论 IRE及冷冻治疗对猪肝组织消融效果良好,而IRE对肝内血管、胆管具有保护作用,可用于肝门、大血管区域的消融。
[Abstract]:Objective to compare and analyze the ablation effect of irreversible electroporation (IRE) and cryotherapy on porcine liver parenchyma. Methods four experimental miniature pigs were randomly divided into 2 groups. The first group was treated with IRE and cryotherapy under the guidance of CT respectively. After 30 days of ablation, the second group was ablated with the same method. Spiral CT plain scan and enhanced scanning were performed during and after operation. After scanning, the two groups of experimental animals were killed and the liver was removed, fixed with 10% formaldehyde solution, and the whole specimen was sectioned for pathological examination. Results the boundary of cryoablation area was clearly defined by CT plain scan during operation and the boundary of ire ablation was not clear by contrast enhanced CT scan, neither IRE nor cryoablation area was enhanced immediately after operation. The boundary of the ablation area was clear, the blood vessel and bile duct were well constructed, the boundary of the frozen ablation area was blurred, and the local bleeding was observed. Conclusion IRE and cryotherapy have a good effect on the ablation of porcine liver tissue, while IRE has protective effect on intrahepatic vessels and bile ducts, and can be used in the ablation of hepatic hilus and large vascular regions.
【作者单位】：中国人民解放军总医院放射科;中国人民解放军海军总医院放射科;
【基金】：国家科技支撑课题(2013BAI17B00)
【分类号】：R81

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