刺梨黄酮对辐射损伤骨髓细胞周期的影响

发布时间：2018-09-14 14:08

【摘要】：目的研究刺梨黄酮(FRT)对辐射损伤骨髓细胞周期的影响。方法取小鼠骨髓细胞制成单细胞悬液,分为空白对照组、辐射组、辐射+50 mg·L~(-1)FRT干预组(50 mg·L~(-1)FRT干预组)、辐射+100 mg·L~(-1)FRT干预组(100 mg·L~(-1)FRT干预组)。空白对照组不加干预;辐射组给予6 Gyγ射线照射;50、100 mg·L~(-1)FRT干预组先在骨髓细胞悬液加入相应量的FRT,使FRT终浓度分别为50、100 mg·L~(-1),2 h后给予6 Gyγ射线照射。经细胞固定、碘化丙啶染色后,进行流式细胞仪检测。结果与空白对照组比较,辐射组照射后6、12 h,G_1期、S期细胞比例均降低(P0.05),G_2期细胞比例均增高(P0.05),且辐射后12 h G_1和S期细胞比例低于6 h时(P0.05),G_2期细胞比例显著高于6 h时(P0.05)。与辐射组比较,50、100 mg·L~(-1)FRT干预组的G_1、S期细胞比例均增高(P0.05),G_2期细胞比例均减少(P0.05),且辐射后6、12 h,100 mg·L~(-1)FRT干预组G_1和S期细胞比例高于50 mg·L~(-1)FRT干预组(P0.05),G_2期细胞比例低于50 mg·L~(-1)FRT干预组(P0.05)。结论 FRT可以降低辐射后骨髓细胞G_2期的细胞比例及增高G_1、S期的细胞所占比例,对γ射线所致骨髓细胞损伤有一定防护作用,且在一定浓度范围内防护效果呈浓度依赖性。
[Abstract]:Objective to study the effect of Rosa roxburghii flavonoids (FRT) on bone marrow cell cycle induced by radiation injury. Methods Mouse bone marrow cells were made into single cell suspension and divided into control group, 50 mg L ~ (-1) FRT intervention group (50 mg L ~ (-1) FRT intervention group) and 100 mg L ~ (-1) FRT intervention group (100 mg L ~ (-1) FRT intervention group). The control group was treated with 6 Gy 纬 -ray irradiation for 50 mg L ~ (-1) FRT, and the bone marrow cell suspension was exposed to the corresponding amount of FRT, for 2 h after the final concentration of FRT was 50100 mg L ~ (-1) ~ (-1) for 2 h. The cells were fixed and stained with pyridine iodide, and detected by flow cytometry. Results compared with the control group, the proportion of S phase cells in the radiation group decreased (P0.05) and the proportion of the cells in the GSP 2 phase increased significantly (P0.05), and at 12 h after irradiation, the proportion of the cells in the G 1 and S phase was lower than that in the 6 h (P0.05). The percentage of the cells in the G 2 phase was significantly higher than that in the 6 h group (P0.05). Compared with the radiation group, the proportion of G1S phase cells in the 50 100 mg L ~ (-1) FRT group was increased (P0.05), and the proportion of G1 and S phase cells in the G1 and S phase group was higher than that in the 50 mg L ~ (-1) FRT intervention group (P0.05). The percentage of G1 and S phase cells in the G1 and S phase group was higher than that in the 50 mg L ~ (-1) FRT intervention group (P0.05). The percentage of G1 and S phase cells in the G1 and S phase group was significantly higher than that in the 50 mg L ~ (-1) FRT intervention group (P0.05). Conclusion FRT can decrease the proportion of bone marrow cells in Gast2 phase after irradiation and increase the proportion of cells in G _ 1 / S phase. It has a protective effect on bone marrow cell injury induced by 纬 -ray, and the protective effect is concentration-dependent within a certain concentration range.
【作者单位】：新乡医学院药学院;
【基金】：新乡医学院研究生科研创新支持计划项目(编号:500111)
【分类号】：R818

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