力竭运动对大鼠心脏传导系统ICAM-1的影响

发布时间：2018-10-07 19:14

【摘要】：目的:探讨力竭运动后不同时相心脏窦房结、房室结和浦肯野氏纤维炎性因子细胞间粘附分子-1(ICAM-1)基因和蛋白水平的表达特点,为运动性心肌损伤与心律失常发生机制的阐明提供实验依据。方法:100只健康成年雄性SD大鼠随机分为10组,每组10只,分别为一次力竭运动组(4组,n=40)、反复力竭运动组(4组,n=40)、一次力竭安静对照组(n=10)和反复力竭安静对照组(n=10)。分别于力竭运动后0、4、12及24小时取材,进行免疫荧光组化及实时荧光定量PCR分析。应用激光显微切割技术定位并收集窦房结、房室结和浦肯野氏纤维细胞,研究细胞炎性因子ICAM-1的m RNA和蛋白表达的变化。结果:一次力竭运动后心脏窦房结ICAM-1 m RNA和蛋白表达在运动后12小时达峰值,显著高于对照组(P0.05),浦肯野氏纤维在运动后24小时达峰值,显著高于对照组(P0.01)。反复力竭运动后心脏窦房结ICAM-1 m RNA和蛋白表达在运动后即刻和12小时出现两次峰值,显著高于对照组(P0.01),浦肯野氏纤维ICAM-1蛋白表达12小时达峰值,显著高于对照组(P0.01)。结论:力竭运动后心脏传导系统不同部位ICAM-1 m RNA和蛋白表达的时相规律各异,但心脏窦房结炎性因子增高幅度较大,是运动性心肌损伤与心律失常的机制之一。
[Abstract]:Objective: to investigate the expression of intercellular adhesion molecule-1 (ICAM-1) gene and protein in different phases of cardiac sinoatrial node, atrioventricular node and Purkinje fibroinflammatory factor after exhaustive exercise. To provide experimental basis for the elucidation of the mechanism of myocardial injury and arrhythmia caused by exercise. Methods one hundred healthy adult male SD rats were randomly divided into 10 groups: one exhaustive exercise group (4 groups), four repeated exhaustive exercise group (4 groups), one exhaustive quiet control group (nn10) and one exhaustive quiet control group (N10). The samples were collected at 12 and 24 hours after exhaustive exercise and analyzed by immunofluorescence histochemistry and real-time fluorescence quantitative PCR. Laser microdissection technique was used to locate and collect sinoatrial node, atrioventricular node and Purkinje fibroblasts to study the expression of m RNA and protein in inflammatory factor ICAM-1. Results: the expression of ICAM-1 m RNA and protein in the sinoatrial node reached a peak at 12 hours after one exhaustive exercise, which was significantly higher than that in the control group (P0.05), and the peak value of Purkinje fiber at 24 hours after exercise was significantly higher than that in the control group (P0.01). After repeated exhaustive exercise, the expression of ICAM-1 m RNA and protein in the sinoatrial node appeared two peaks immediately after exercise and 12 hours after exercise, which was significantly higher than that in the control group (P0.01). The expression of ICAM-1 protein in Purkinje fiber reached its peak at 12 hours and was significantly higher than that in the control group (P0.01). Conclusion: after exhaustive exercise, the expression of ICAM-1 m RNA and protein in different parts of cardiac conduction system is different, but the increase of inflammatory factors in sinoatrial node is larger, which is one of the mechanisms of myocardial injury and arrhythmia induced by exercise.
【作者单位】：国家体育总局体育科学研究所;上海体育学院;
【基金】：国家体育总局体育科学研究所基本科研项目14-01
【分类号】：R87

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