γ电离辐射对斑马鱼胚胎肝、脾和肾发育形态学的影响
发布时间：2018-10-10 07:58
【摘要】：为研究γ电离辐射对水生生物重要器官发育形态学的影响,以斑马鱼(Danio rerio)作为模式生物,研究了胚胎发育早期(受精后5h,5hpf)接受急性γ辐照(累积剂量为0.25、0.5、1Gy,剂量率为47.79cGy/min)后,受精后3d(3dpf)的幼鱼卵黄囊面积以及3dpf和5dpf的幼鱼肝、肾和脾发育形态学的变化。结果显示:3dpf的幼鱼卵黄囊面积随剂量的增大而增大;3dpf的幼鱼肝脏中空泡增加、肝脏局部出现空洞;5dpf的幼鱼肝脏尺寸缩减、肝细胞内空泡增加且细胞核变形、血窦较小以及有核红细胞变形等;但前肾和脾的发育形态并没有出现异常。随后对3dpf的幼鱼肝细胞超微结构进行观察,发现γ辐照使幼鱼肝脏细胞线粒体受损、核膜间隙扩大、粗面内质网排列混乱等。以上结果表明,γ辐照对胚胎卵黄吸收有抑制作用,并呈一定的剂量依赖性,胚胎肝脏的发育形态在显微及亚显微结构上都发生了改变。
[Abstract]:In order to study the effect of 纬 ionizing radiation on the development morphology of important organs of aquatic organisms, zebrafish (Danio rerio) was used as model organism to study the acute 纬 irradiation (cumulative dose 0.25g 0.51Gy, dose rate 47.79cGy/min) in early embryonic development (5hu 5hpf after fertilization). The area of yolk sac of young fish (3dpf) and the morphologic changes of liver kidney and spleen of 3dpf and 5dpf were observed on the 3rd day after fertilization. The results showed that the area of yolk sac increased with the increase of dose, the vacuole of liver of 3 dpf increased, the size of liver of 5dpf decreased, the vacuole of liver cells increased and the nucleus of liver deformed. The antrum was small and the nucleated red blood cells deformed, but the shape of the anterior kidney and spleen was not abnormal. The ultrastructure of hepatocytes of 3dpf was observed. It was found that 纬 irradiation damaged mitochondria, enlarged nuclear membrane space and confused rough endoplasmic reticulum arrangement. The results showed that 纬 irradiation inhibited yolk absorption in a dose-dependent manner, and the developmental morphology of the embryonic liver changed in both microscopic and submicroscopic structures.
【作者单位】： 南华大学铀矿冶生物技术国防重点学科实验室;
【基金】：国防基础重点基金资助项目(B3720132001) 国家自然科学基金重点项目资助(U1401231) 国防基础科研计划资助项目(JCKY2016403C001)
【分类号】：R818
，
本文编号：2261249
[Abstract]:In order to study the effect of 纬 ionizing radiation on the development morphology of important organs of aquatic organisms, zebrafish (Danio rerio) was used as model organism to study the acute 纬 irradiation (cumulative dose 0.25g 0.51Gy, dose rate 47.79cGy/min) in early embryonic development (5hu 5hpf after fertilization). The area of yolk sac of young fish (3dpf) and the morphologic changes of liver kidney and spleen of 3dpf and 5dpf were observed on the 3rd day after fertilization. The results showed that the area of yolk sac increased with the increase of dose, the vacuole of liver of 3 dpf increased, the size of liver of 5dpf decreased, the vacuole of liver cells increased and the nucleus of liver deformed. The antrum was small and the nucleated red blood cells deformed, but the shape of the anterior kidney and spleen was not abnormal. The ultrastructure of hepatocytes of 3dpf was observed. It was found that 纬 irradiation damaged mitochondria, enlarged nuclear membrane space and confused rough endoplasmic reticulum arrangement. The results showed that 纬 irradiation inhibited yolk absorption in a dose-dependent manner, and the developmental morphology of the embryonic liver changed in both microscopic and submicroscopic structures.
【作者单位】： 南华大学铀矿冶生物技术国防重点学科实验室;
【基金】：国防基础重点基金资助项目(B3720132001) 国家自然科学基金重点项目资助(U1401231) 国防基础科研计划资助项目(JCKY2016403C001)
【分类号】：R818
，
本文编号：2261249
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