酪氨酸羟化酶基因SNP多态性与运动性失眠的实验研究

发布时间：2018-10-10 13:32

【摘要】：运动性失眠是失眠症的一种特殊类型，是运动性疲劳的症状之一。适度的运动有助于睡眠，但是超过一定的限度就有可能导致失眠即运动性失眠。关于失眠是否存在一定的遗传性，，曾有研究人员通过调查并证实了失眠的遗传性，儿茶酚胺是人体内重要激素及神经递质，由酪氨酸合成而来，合成过程中有四种酶参与，酪氨酸羟化酶(tyrosine hydroxylase)是第一个酶，催化酪氨酸生成多巴，在调节中枢神经和交感神经系统功能上发挥着重要的作用，由于TH在调节中枢神经中的重要作用，本文预从TH基因水平来探讨运动性失眠发生。目的：通过问卷调查、PCR技术测序，分析酪氨酸羟化酶基因SNP基因分型在失眠组与非失眠组统计学差异，探讨酪氨酸羟化酶基因多态性与运动性失眠发生是否存在一定的相关性。方法：用问卷调查法对成都体育学院体育系运动系的篮球班、武术班、健美操班、举重班、田径班、篮球队、武术队、健美操队、举重队、田径队430名运动员进行主观感觉调查，按照运动性失眠的评定标准进行分析判断，把受试者分成失眠组与非失眠组，应用聚合酶链反应一限制性片段长度多态性(PcR-RFLP)的分析方法，通过测序得到失眠组16人的酪氨酸羟化酶基因序列和非失眠组18人的酪氨酸羟化酶基因序列。通过比较失眠组与非失眠组酪氨酸羟化酶基因SNP多态性，来判断酪氨酸羟化酶基因是否与运动性失眠的发生存在一定的联系。结果：1、运动员疲劳自觉症状测定，其中67%的运动员得分处于第四个等级（19分-24分）。2、篮球、武术、健美操、举重、田径5个项目的运动员运动训练后举重运动员100%出现形体疲劳，20%出现脏腑疲劳，9%出现神志疲劳；篮球、武术、健美操、田径出现神志疲劳的概率分别为：60%、52%、50%、36%。3、从实验数据分析酪氨酸羟化酶基因位点G-494A、C-388T、A-641G的分型在运动性失眠组与非失眠组存在统计学差异，而G-94T、A-581G、T-741C、G-801C、G-814A、C-824T和C-833T等7个位点统计结果无统计学差异。结论：1、运动训练后67%的运动员会出现中高等程度的疲劳。2、举重运动员出现形体疲劳比率较大，出现脏腑疲劳和神志疲劳的比率较小。篮球、武术、健美操、田径项目的运动员出现脏腑疲劳和神志疲劳的比率较大。3、酪氨酸羟化酶基因位点G-494A、C-388T、A-641G的多态性与运动性失眠的发生存在一定的相关迹象，但是由于采集的样本量大少还不能证明酪氨酸羟化酶基因位点G-494A、C-388T、A-641G与运动性失眠存在确切相关,同时酪氨酸羟化酶基因G-94T、A-581G、T-741C、G-801C、G-814A、C-824T和C-833T等7个位点在本实验结果中没有出现与运动性失眠相关的迹象。
[Abstract]:Exercise insomnia is a special type of insomnia and one of the symptoms of exercise fatigue. Moderate exercise helps sleep, but beyond a certain limit can lead to insomnia, or exercise insomnia. As to whether insomnia is hereditary or not, some researchers have investigated and confirmed the heredity of insomnia. Catecholamine is an important hormone and neurotransmitter in the human body, which is synthesized by tyrosine and involved in the synthesis of four enzymes. Tyrosine hydroxylase (tyrosine hydroxylase) is the first enzyme to catalyze tyrosine to produce dopa, which plays an important role in regulating the function of central nervous system and sympathetic nervous system, because of the important role of TH in regulating central nervous system. In this study, TH gene level was used to study the occurrence of motor insomnia. Objective: to analyze the statistical difference of tyrosine hydroxylase gene SNP genotyping between insomnia group and non-insomnia group by questionnaire investigation and PCR sequencing, and to explore whether there is a certain correlation between tyrosine hydroxylase gene polymorphism and motor insomnia. Methods: the basketball class, martial arts class, aerobics class, weightlifting class, track and field class, basketball team, martial arts team, calisthenics team, weightlifting team, and weightlifting team were investigated by questionnaire in Department of physical Education of Chengdu Institute of physical Education. Three hundred and thirty athletes of track and field team investigated their subjective feelings, analyzed and judged according to the evaluation criteria of motor insomnia, divided them into insomnia group and non-insomnia group, and used polymerase chain reaction-restriction fragment length polymorphism (PcR-RFLP) analysis method. The tyrosine hydroxylase gene sequences of 16 patients with insomnia and 18 patients with no insomnia were obtained by sequencing. By comparing the SNP polymorphism of tyrosine hydroxylase gene between insomnia group and non-insomnia group, the relationship between tyrosine hydroxylase gene and motor insomnia was determined. Results: 1, the athletes' fatigue conscious symptoms were measured, of which 67% of the athletes scored in the fourth grade (19-24 points). 2, basketball, martial arts, aerobics, weightlifting, 100% of weightlifters developed physical fatigue, 20% viscera fatigue, 9% mental fatigue, basketball, martial arts, calisthenics, The probability of mental fatigue in track and field were as follows: 60: 52and 50: 360.The tyrosine hydroxylase gene locus G-494AnC-388TFA-641G was analyzed from the experimental data. There was statistical difference between the exercise insomnia group and the non-insomnia group, but there was no statistical difference between the G-94 TA- 581GT-741GT-801C741 G-814AHN C-824T and C-833T loci. Conclusion: 1. After training, 67% of athletes will develop middle and higher degree fatigue. 2. Weight lifters have higher body fatigue rate, and lower ratio of viscera fatigue and mental fatigue. Athletes in basketball, martial arts, aerobics and track and field events have higher rates of viscera fatigue and mental fatigue. 3. The polymorphism of tyrosine hydroxylase gene locus G-494AnC-388TKA-641G is associated with the occurrence of sports insomnia. However, because the sample size was large and small, the tyrosine hydroxylase gene locus G-494An C-388TKA-641G could not be proved to be related to motor insomnia. At the same time, 7 loci of tyrosine hydroxylase gene G-94 TX A-581GFT-741CU G-801CU G-814ANC-824T and C-833T did not show any correlation with motor insomnia in this study.
【学位授予单位】：成都体育学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R87

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