MALDI-TOF-MS对DAI大鼠脑干蛋白质组学的分析

发布时间：2018-10-12 17:38

【摘要】：目的应用基质辅助激光解吸/电离-飞行时间质谱(matrix-assisted laser desorption/ionization timeof-flight mass spectrometry,MALDI-TOF-MS)技术建立弥漫性轴索损伤(diffuse axonal injury,DAI)诊断模型,筛选DAI相关蛋白或多肽,为寻找DAI的分子标志物提供理论基础。方法将15只雄性SD大鼠随机分为DAI组(n=10)和对照组(n=5),采用MALDI-TOF-MS技术对两组大鼠脑干组织的蛋白或多肽表达谱进行检测,运用Clin Pro Tools 2.2分析软件筛选出具有差异的分子峰,应用遗传算法建立诊断模型。结果筛选出有差异的分子峰61个(P0.05),其中DAI组有9个分子峰下调,52个分子峰上调。筛选出5个分子峰建立诊断模型,交叉验证的特异性为96.14%、敏感性为95.98%,盲法验证结果的特异性为73.33%、敏感性为70.00%。结论应用MALDI-TOF-MS技术不仅能够建立特异性和敏感性较高的DAI诊断模型,还可以筛选差异蛋白或多肽,为DAI的法医学鉴定奠定理论基础。
[Abstract]:Objective to establish a diagnostic model of diffuse axonal injury (diffuse axonal injury,DAI) by matrix assisted laser desorption / ionization / time of flight mass spectrometry (matrix-assisted laser desorption/ionization timeof-flight mass spectrometry,MALDI-TOF-MS), and to screen DAI related proteins or peptides, and to provide a theoretical basis for searching for molecular markers of DAI. Methods Fifteen male SD rats were randomly divided into DAI group (n = 10) and control group (n = 5). The expression profiles of protein or polypeptide in brain stem tissue of two groups were detected by MALDI-TOF-MS technique. The molecular peaks were screened by Clin Pro Tools 2.2 analysis software. Genetic algorithm is used to establish diagnostic model. Results there were 61 different molecular peaks (P0.05). In DAI group, there were 9 down-regulated molecular peaks and 52 up-regulated molecular peaks. Five molecular peaks were screened to establish diagnostic models. The specificity of cross-validation was 96.14, the sensitivity was 95.98, the specificity of blind test was 73.33, and the sensitivity was 70.00. Conclusion MALDI-TOF-MS can not only establish a specific and sensitive diagnostic model for DAI, but also screen differentially expressed proteins or peptides, thus laying a theoretical foundation for forensic identification of DAI.
【作者单位】：南方医科大学基础医学院法医学系;司法部司法鉴定科学技术研究所上海市法医学重点实验室;
【基金】：中央级科研院所科研专项(GY2013Z-3,GY2015G-2)
【分类号】：D919

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