大鼠尺骨活体疲劳损伤后微损伤变化规律研究
发布时间：2018-10-13 13:36
【摘要】：目的：比较活体大鼠尺骨疲劳损伤实验后骨微破裂指标,确定建立活体大鼠皮质骨微损伤模型参数指标。 方法：根据四点弯曲原理,我们课题小组研制出与PLD-5010型疲劳损伤实验机相配套的平行四点弯曲夹具,应用于疲劳损伤实验。选取40只7月龄雌性SD大鼠(由湘雅二医院动物实验室提供),体重260±15.6g,置于动物实验室饲养1周后,随机分为去卵巢组(OVX组)和假手术组(SHAM组),每组均20只。OVX组和SHAM组分别在手术后3周后,应用PLD-5010型疲劳损伤实验机及四点弯曲夹具对大鼠右侧尺骨中段行疲劳损伤试验。其中将OVX组、SHAM组根据疲劳时间长短分为OVX1W组,OVX2W组,SHAM1W组,SHAM2W组。最后将所有大鼠麻醉后放血处死,取右侧尺骨行大块组织碱性品红染色、塑料包埋和磨片,测量骨微破裂各指标和骨细胞密度等。 结果：各组微损伤形态比较：各组微破裂形态主要表现为微破裂。各组之间微破裂相关指标比较：同一疲劳时间不同处理组,OVX组中微破裂长度、微破裂数量、微破裂密度、微破裂面密度相关指标数值均高于SHAM组,有统计学差异(P0.05)；同一处理组不同疲劳时间,OVX2W组微破裂长度、微破裂面密度指标数值均高于OVX1W组,有统计学差异(P0.05),微破裂数量及微破裂密度指标数值无统计学差异(P0.05)；SHAM1W组与SHAM2W组之间微破裂相关指标无统计学差异(P0.05)。各组骨细胞密度比较：OVX组均高于SHAM组,有统计学差异(P0.05)；OVX组之间有统计学差异(P0.05)；SHAM组之间无统计学差异(P0.05)。 结论：应用PLD-5010型疲劳损伤实验机及平行四点弯曲夹具对大鼠尺骨行疲劳损伤实验,能够建立活体大鼠尺骨疲劳损伤模型；确定建立模型参数为每次最大载荷0.0555N/g体重,频率4Hz,负载次数10000次,时间为2周,1周3次。 目的：建立大鼠皮质骨微损伤模型后,观测大鼠尺骨疲劳后不同时间骨微损伤变化,初步探讨大鼠微损伤自身修复规律。 方法：根据第一部分实验结果建立骨质疏松疲劳微损伤模型参数,选取40只7月龄大鼠随机分为4组,并将平行四点弯曲单夹具改良为双夹具,予PLD-5010型疲劳损伤实验机及平行四点弯曲夹具对大鼠尺骨行疲劳损伤实验,疲劳时间为2周。每组分别于疲劳损伤后1周、2周、3周以及4周处死,取大鼠右侧尺骨,行大块组织碱性品红染色、塑料包埋和磨片,磨片测量骨微破裂指标。 结果：各组之间微破裂相关指标比较：各组微破裂形态主要表现为微破裂。OVX1W组与OVX2W组微破裂长度、微破裂数量、微破裂密度及微破裂面密度明显高于OVX3W组及OVX4W组,具有统计学差异(P0.05)；OVX1W与OVX2W之间微破裂相关指标数值无明显差异性(P0.05),OVX3W及OVX4W微破裂相关指标数值无明显差异性(P0.05)。 结论：疲劳实验结束后,大鼠右侧尺骨微破裂数量及长度随时间变化明显减少,2周到3周之间修复最为明显,4周时骨微破裂逐渐修复。
[Abstract]:Aim: to compare the indexes of bone microrupture after ulnar fatigue injury in living rats and to determine the parameters of cortical microinjury model in vivo. Methods: according to the four-point bending principle, our team developed a parallel four-point bending fixture matched with PLD-5010 fatigue damage testing machine, which was applied to fatigue damage experiment. Forty 7-month-old female SD rats (provided by the animal laboratory of Xiangya No.2 Hospital), weighing 260 卤15.6g, were placed in the animal laboratory for one week. Two groups were randomly divided into ovariectomized group (OVX group) and sham operation group (SHAM group) with 20 rats in each group. After 3 weeks of operation, the OVX group and SHAM group were subjected to the fatigue injury test of the middle segment of the right ulna using PLD-5010 type fatigue injury test machine and four-point bending clamp respectively. OVX group and SHAM group were divided into OVX1W group, OVX2W group, SHAM1W group and SHAM2W group according to fatigue time. At last, all the rats were bled out and killed after anesthesia. The right ulna was stained by alkaline fuchsin staining, plastic embedding and grinding, and the indexes of bone microrupture and the density of bone cells were measured. Results: the microfracturing morphology of each group was mainly microruptured. Comparison of microfracture correlation indexes among groups: the length, number, density and density of microrupture in OVX group were significantly higher than those in SHAM group with the same fatigue time (P0.05). Different fatigue time of the same treatment group, OVX2W group microfracture length, microfracture surface density index values were higher than OVX1W group, there was statistical difference (P0.05), the number of micro-rupture and micro-rupture density index values were not statistically different (P0.05); There was no statistical difference between SHAM1W group and SHAM2W group (P0.05). Comparison of bone cell density in each group: OVX group was higher than SHAM group, there was statistical difference (P0.05); OVX group there was statistical difference (P0.05); SHAM group there was no statistical difference (P0.05). Conclusion: the fatigue injury model of ulna of rats can be established by using PLD-5010 fatigue injury test machine and parallel four-point bending fixture, and the model parameters are determined to be maximum load 0.0555N/g weight per time. Frequency 4 Hz, load times 10000 times, time 2 weeks, 3 times a week. Aim: to observe the changes of bone microdamage after ulnar fatigue in rats and to explore the self-repair rule of microinjury in rats. Methods: according to the experimental results of the first part, the model parameters of osteoporosis fatigue microdamage were established. Forty 7-month-old rats were randomly divided into 4 groups, and the parallel four-point bending single clamp was modified into two clamps. The fatigue damage test of ulna of rats was carried out with PLD-5010 type fatigue damage test machine and parallel four point bending fixture. The fatigue time was 2 weeks. Each group was killed at 1 week, 2 weeks, 3 weeks and 4 weeks after fatigue injury. The right ulna of the rats were collected and stained with alkaline fuchsin. Results: the results showed that the microrupture morphology of each group was mainly microrupture, the length, number, density and surface density of microrupture in OVX1W group and OVX2W group were significantly higher than those in OVX3W group and OVX4W group. There was statistical difference (P0.05); there was no significant difference between OVX1W and OVX2W (P0.05); there was no significant difference between OVX3W and OVX4W (P0.05). Conclusion: after fatigue test, the number and length of right ulnar microrupture in rats decreased obviously with time, the repair was most obvious between 2 weeks and 3 weeks, and gradually repaired at 4 weeks.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R87
本文编号：2268836
[Abstract]:Aim: to compare the indexes of bone microrupture after ulnar fatigue injury in living rats and to determine the parameters of cortical microinjury model in vivo. Methods: according to the four-point bending principle, our team developed a parallel four-point bending fixture matched with PLD-5010 fatigue damage testing machine, which was applied to fatigue damage experiment. Forty 7-month-old female SD rats (provided by the animal laboratory of Xiangya No.2 Hospital), weighing 260 卤15.6g, were placed in the animal laboratory for one week. Two groups were randomly divided into ovariectomized group (OVX group) and sham operation group (SHAM group) with 20 rats in each group. After 3 weeks of operation, the OVX group and SHAM group were subjected to the fatigue injury test of the middle segment of the right ulna using PLD-5010 type fatigue injury test machine and four-point bending clamp respectively. OVX group and SHAM group were divided into OVX1W group, OVX2W group, SHAM1W group and SHAM2W group according to fatigue time. At last, all the rats were bled out and killed after anesthesia. The right ulna was stained by alkaline fuchsin staining, plastic embedding and grinding, and the indexes of bone microrupture and the density of bone cells were measured. Results: the microfracturing morphology of each group was mainly microruptured. Comparison of microfracture correlation indexes among groups: the length, number, density and density of microrupture in OVX group were significantly higher than those in SHAM group with the same fatigue time (P0.05). Different fatigue time of the same treatment group, OVX2W group microfracture length, microfracture surface density index values were higher than OVX1W group, there was statistical difference (P0.05), the number of micro-rupture and micro-rupture density index values were not statistically different (P0.05); There was no statistical difference between SHAM1W group and SHAM2W group (P0.05). Comparison of bone cell density in each group: OVX group was higher than SHAM group, there was statistical difference (P0.05); OVX group there was statistical difference (P0.05); SHAM group there was no statistical difference (P0.05). Conclusion: the fatigue injury model of ulna of rats can be established by using PLD-5010 fatigue injury test machine and parallel four-point bending fixture, and the model parameters are determined to be maximum load 0.0555N/g weight per time. Frequency 4 Hz, load times 10000 times, time 2 weeks, 3 times a week. Aim: to observe the changes of bone microdamage after ulnar fatigue in rats and to explore the self-repair rule of microinjury in rats. Methods: according to the experimental results of the first part, the model parameters of osteoporosis fatigue microdamage were established. Forty 7-month-old rats were randomly divided into 4 groups, and the parallel four-point bending single clamp was modified into two clamps. The fatigue damage test of ulna of rats was carried out with PLD-5010 type fatigue damage test machine and parallel four point bending fixture. The fatigue time was 2 weeks. Each group was killed at 1 week, 2 weeks, 3 weeks and 4 weeks after fatigue injury. The right ulna of the rats were collected and stained with alkaline fuchsin. Results: the results showed that the microrupture morphology of each group was mainly microrupture, the length, number, density and surface density of microrupture in OVX1W group and OVX2W group were significantly higher than those in OVX3W group and OVX4W group. There was statistical difference (P0.05); there was no significant difference between OVX1W and OVX2W (P0.05); there was no significant difference between OVX3W and OVX4W (P0.05). Conclusion: after fatigue test, the number and length of right ulnar microrupture in rats decreased obviously with time, the repair was most obvious between 2 weeks and 3 weeks, and gradually repaired at 4 weeks.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R87
【参考文献】
相关期刊论文 前2条
1 陈波;戴如春;戴哲浩;章婵;张灿;张喜;吴博;张玲;伍贤平;廖二元;;活体大鼠尺骨四点弯曲疲劳试验夹具的研制与应用[J];中华骨质疏松和骨矿盐疾病杂志;2011年01期
2 戴如春,廖二元,杨川,伍贤平,彭健;骨微破裂评价去卵巢大鼠骨结构与质量作用[J];湖南医科大学学报;2003年06期
，本文编号：2268836
本文链接：https://www.wllwen.com/yixuelunwen/yundongyixue/2268836.html
最近更新
教材专著
