不同剂量X射线辐射对睾丸生物学效应的影响

发布时间：2018-10-29 09:05

【摘要】：目的:观察不同剂量X射线全身照射对西藏小型猪睾丸生物学效应的影响。方法:18头成年雄性西藏小型猪随机分为6组。实验组5组,对照组1组。实验组分别接受2、5、8、11、14 Gy全身X线照射。照射72 h后观察血清睾酮、精子畸形率、生殖细胞DNA损伤、睾丸组织学变化。结果:血清睾酮5、8、11、14Gy组与对照组比较差异有显著性(P0.05),自5 Gy起随着辐射剂量的增加,血清睾酮浓度降低,各组间差异有显著性(P0.05);精子畸形率2、5 Gy组别随着辐射剂量的增加明显增高(P0.01),8、11、14 Gy组别,管腔内已无成熟精子;尾部DNA百分率和尾距随着辐射剂量增加而增加,各组间差异有显著性(P0.05),存在着明显的剂量-效应关系。睾丸病理形态显示随剂量增加,生精上皮明显变薄,生精细胞剥离脱落,空洞形成,管腔已无成熟精子。结论:X射线辐射后可引起雄性西藏小型猪生殖损伤,具有明显的剂量效应。5 Gy是对睾丸组织生理损伤的关键点。生殖细胞DNA损伤率可作为一种剂量估算的指标。
[Abstract]:Objective: to observe the effects of different doses of X-ray irradiation on testis biological effects of Tibet miniature pigs. Methods: eighteen adult male Tibetan miniature pigs were randomly divided into 6 groups. The experimental group (n = 5) and the control group (n = 1). The experimental group were exposed to whole body X-ray irradiation for 2 d, 5 d, 8, 111, 14 Gy. After 72 hours of irradiation, serum testosterone, sperm malformation rate, DNA damage of germ cells and histological changes of testis were observed. Results: compared with the control group, there was a significant difference in serum testosterone (P 0.05). The serum testosterone concentration decreased with the increase of radiation dose from 5 Gy onwards (P0.05). The sperm deformity rate increased obviously with the increase of radiation dose (P0.01) in group 2 and 5 Gy, and in group 81114 Gy, there was no mature sperm in the lumen. The percentage of tail DNA and tail distance increased with the increase of radiation dose, there was significant difference among the groups (P0.05), there was a significant dose-effect relationship. Testicular histopathology showed that the spermatogenic epithelium thinned obviously with the increase of dosage, the spermatogenic cells were stripped off, cavities formed, and no mature sperm was found in the lumen. Conclusion: the reproductive injury of male Tibetan miniature pigs can be induced by X ray irradiation and has a significant dose effect. 5 Gy is the key point of physiological injury to testis. The DNA damage rate of germ cells can be used as a dose estimation index.
【作者单位】：南方医科大学实验动物中心;南方医科大学珠江医院泌尿外科;
【基金】：国家自然科学基金项目(编号:81402625) 广东省自然科学基金项目(编号:S2013010014720) 中国博士后科学基金项目(编号:2014M550439)
【分类号】：R818

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