高脂膳食对大鼠心肌的脂毒性作用及有氧运动、原花青素干预效应的实验研究

发布时间：2018-11-05 13:01

【摘要】：目的:通过对高脂膳食联合有氧运动以及补充原花青素大鼠血浆FFA、IL-6、TNF-α水平变化和心肌TLR4、NF-κBm RNA表达量、FFA、ICAM-1含量变化的分析,探讨高脂膳食对大鼠心肌的脂毒性作用及有氧运动联合补充原花青素对高脂膳食大鼠心肌的保护作用。方法:8周龄健康SD大鼠50只,体重180-220克。适应性喂养1周后随机分为5组:对照组(N=10,C)、高脂膳食组(N=10,H)、高脂膳食运动组(N=10,HE)、高脂膳食补充原花青素组(N=10,HG)、高脂膳食运动补充原花青素组(N=10,HEG)。HE、HEG组进行有氧运动,均采用0坡度跑台,第一周15m/min,第二周20m/min,以后每周增加1m/min,运动50min/次。运动时间:21:00-24:00,1次/天,6天/周,共6周。HG、HEG组每运动前半小时按200mg/kg体重原花青素灌胃,其余组等量等体积生理盐水灌胃。每周固定时间测量记录各组体重体长数据。6周实验结束后,ELISA法检测各组大鼠血浆FFA、IL-6、TNF-α水平和心肌FFA、ICAM-1的含量,RT-PCR方法测试心肌NF-κBm RNA表达水平,Western blot法测TLR4蛋白表达量。运用SPSS17.0软件处理数据。结果:1.H组大鼠Lee’s指数、血浆FFA、TNF-α、IL-6含量和心肌TLR4、NF-κBm RNA表达水平及FFA、ICAM-1含量明显高于C组(P0.01)。2.HE、HG、HEG组大鼠Lee’s指数、血浆FFA、TNF-ɑ含量明显低于H组(P0.01、P0.05),血浆IL-6水平无显著性变化(P0.05);有氧运动、补充原花青素对Lee’s指数、血浆FFA、TNF-ɑ含量的影响无显著交互作用(P0.05)。3.HE、HG、HEG组大鼠心肌ICAM-1含量与H组相比无显著差异(P0.05),有氧运动、补充原花青素对心肌ICAM-1含量的影响无显著交互作用(P0.05)。4.HE、HG、HEG组大鼠心肌FFA、TLR4蛋白含量和NF-кBm RNA表达量明显低于H组(P0.05、P0.01);有氧运动、补充原花青素对心肌FFA、TLR4蛋白含量和NF-кBm RNA表达量的影响有显著交互作用(P0.05);在相同补充原花青素的条件下,运动可使大鼠FFA含量明显降低(P0.05);相同运动条件下,补充原花青素可使大鼠TLR4、NF-кBm RNA表达量明显降低(P0.01)。结论:1.6周高脂膳食可使大鼠体重和Lee’s指数增加,血浆FFA、TNF-ɑ、IL-6水平不同程度的升高,诱发机体脂毒性和炎症反应。2.6周高脂膳食可使大鼠心肌FFA、TLR4和ICAM-1含量增加、NF-κBm RNA表达上调,诱发心肌脂毒性和炎症反应以及心肌血管内皮损伤。3.6周有氧运动和补充原花青素具有减轻高脂膳食大鼠肥胖、缓解其机体和心肌脂毒性及炎症反应作用,从而保护心肌;有氧运动联合补充原青花素对心肌的保护作用比较单一有氧运动或补充原青花素效果更优。
[Abstract]:Objective: to analyze the changes of plasma FFA,IL-6,TNF- 伪 level and myocardial TLR4,NF- 魏 Bm RNA expression and FFA,ICAM-1 content in rats with high fat diet combined with aerobic exercise and procyanidin supplementation. To investigate the effects of hyperlipidemic diet on myocardial lipid toxicity in rats and the protective effects of aerobic exercise combined with procyanidin supplementation on myocardium of rats with high fat diet. Methods: 50 healthy 8-week SD rats with body weight of 180-220 g were used. After one week of adaptive feeding, the rats were randomly divided into five groups: control group (n = 10), high fat diet group (n = 10) and proanthocyanidin supplement group (n = 10 HG). High fat diet supplemented with proanthocyanidin (NC10). The HE,HEG group performed aerobic exercise with 0 slope running platform, 15 m / min in the first week, 20 m / min in the second week, and 1 m / min, 1 m / min, 50min/ times per week thereafter. Exercise time: 21: 00-24: 00 once a day, 6 days a week for 6 weeks. The HG,HEG group was gavaged with 200mg/kg proanthocyanidins half an hour before exercise, and the other groups were given the same volume of normal saline. The body weight and body length of each group were measured at a fixed time every week. At the end of the 6-week experiment, the plasma FFA,IL-6,TNF- 伪 level and myocardial FFA,ICAM-1 content were measured by ELISA method. The expression level of NF- 魏 Bm RNA in myocardium was measured by RT-PCR method. The expression of TLR4 protein was measured by, Western blot method. Use SPSS17.0 software to process data. Results: 1. The Lee's index, plasma FFA,TNF- 伪, IL-6 content, myocardial TLR4,NF- 魏 Bm RNA expression and FFA,ICAM-1 content in H group were significantly higher than those in C group (P0.01). 2. The level of plasma FFA,TNF- was significantly lower than that of group H (P 0.01 P 0.05), but the level of plasma IL-6 had no significant change (P 0.05). There was no significant interaction between aerobic exercise and procyanidin supplementation on Lee's index and plasma FFA,TNF- content (P0.05). 3. There was no significant difference in myocardial ICAM-1 content between HGG group and H group (P0.05). The effects of aerobic exercise and proanthocyanidin supplementation on myocardial ICAM-1 content were not significant (P0.05). 4. The content of FFA,TLR4 protein and the expression of NF- Bm RNA in myocardium of HEHG group were significantly lower than that of H group (P0.05). P0.01); The effects of aerobic exercise and proanthocyanidin supplementation on myocardial FFA,TLR4 protein content and NF- Bm RNA expression were significant (P0.05). Under the same condition of supplementation of proanthocyanidins, the content of FFA in rats was significantly decreased by exercise (P0.05), and the expression of TLR4,NF- Bm RNA in rats was significantly decreased by supplementation of proanthocyanidins under the same exercise conditions (P0.01). Conclusion: the high fat diet of 1.6 weeks can increase the body weight and Lee's index of rats, and increase the levels of plasma FFA,TNF- and IL-6 in varying degrees, which can induce lipid toxicity and inflammatory reaction. 2.6-week high fat diet can induce myocardial FFA, in rats. The contents of TLR4 and ICAM-1 were increased, the expression of NF- 魏 Bm RNA was upregulated, myocardial lipotoxicity and inflammation were induced, and myocardial vascular endothelial injury was induced. Aerobic exercise and proanthocyanidin supplementation at 3.6 weeks could reduce obesity in high-fat diet rats. Alleviating the lipid toxicity and inflammatory reaction of the body and myocardium, thereby protecting the myocardium; The protective effect of aerobic exercise combined with procyanidin on myocardium was better than that of single aerobic exercise or proanthocyanidin supplementation.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R87

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