有氧运动对D-半乳糖诱导的脑衰老大鼠前额叶SYP、PSD-95表达的影响

发布时间：2018-11-06 19:24

【摘要】：目的:观察有氧运动对D-半乳糖(D-gal)诱导的脑衰老大鼠大脑前额叶突触素(SYP)、突触后致密区蛋白-95(PSD-95)表达的影响,探讨有氧运动延缓脑衰老时学习记忆障碍的潜在机制。方法:将36只3月龄SD大鼠随机分成生理盐水对照组(C组)、D-gal组(D组)和D-gal加有氧运动组(DE组),每组12只。每日给予D、DE组大鼠1次腹腔注射D-gal(100 mg/kg/d),持续6周;DE组大鼠在D-gal注射期间进行游泳训练(1 h/d,6 d/w)。然后利用Morris水迷宫检测大鼠空间学习记忆能力,并采用免疫荧光、Western Blot和Real Time-PCR技术分别检测大鼠大脑前额叶SYP、PSD-95蛋白分子及其m RNA表达水平。结果:(1)在Morris水迷宫实验定位航行训练过程中各大鼠的逃避潜伏期均逐渐下降,其中C和DE组训练第3天表现出最好成绩,其后无显著性改变,D组逃避潜伏期则在第5天后降到最低,在第2、3、4天,D组平均逃避潜伏期明显长于C组和DE组(P0.05),而在第1、5、6天,各组大鼠间无显著性差异(P0.05);在空间探索实验中,D组60 s内穿越原平台区域的次数明显低于C和DE组(P0.05),而且后两组大鼠有更长时间停留在原平台所在象限内(P0.05)。(2)与C组相比,D组大鼠前额叶SYP蛋白分子及其SYP m RNA表达水平均显著性降低(P0.01),DE组则显著性高于D组(P0.05);D组大鼠前额叶PSD-95蛋白分子及其m RNA较C组显著性下降(P0.01,P0.05);DE组PSD-95 m RNA与D组无显著性差异,但其表达水平有所上升,并且DE组PSD-95蛋白分子显著性高于D组(P0.05)。结论:有氧运动可在一定程度上抑制D-gal诱导的衰老过程中大鼠前额叶突触蛋白SYP、PSD-95表达受损,减轻其下降程度,这可能是有氧运动延缓脑衰老学习记忆能力下降的分子机理之一。
[Abstract]:Aim: to observe the effect of aerobic exercise on the expression of presynaptic protein 95 (PSD-95) in prefrontal synaptophysin (SYP), in brain of aging rats induced by D-galactose (D-gal). To explore the potential mechanism of learning and memory impairment during aerobic exercise delaying brain aging. Methods: Thirty-six 3-month-old SD rats were randomly divided into normal saline control group (group C), D-gal group (group D) and D-gal plus aerobic exercise group (DE group) with 12 rats in each group. Rats in the DE group were treated with intraperitoneal injection of D-gal (100 mg/kg/d) daily for 6 weeks, while rats in the DE group underwent swimming training during D-gal injection (1 h / d / 6 d / w). Then the spatial learning and memory ability of rats was measured by Morris water maze, and the expression of SYP,PSD-95 protein and m RNA in the prefrontal lobe of rat brain were detected by immunofluorescence, Western Blot and Real Time-PCR techniques, respectively. Results: (1) the escape latency of the rats in the Morris water maze experiment decreased gradually during the navigation training. Group C and group DE showed the best results on the 3rd day of training, but there was no significant change after that. The average escape latency of group D was significantly longer than that of group C and DE (P0.05), but there was no significant difference between the two groups (P0.05). In the space exploration experiment, the times of crossing the original platform area in group D within 60 seconds were significantly lower than those in group C and DE (P0.05), and the latter two groups had a longer stay in the quadrant of the original platform (P0.05). (2) compared with group C. The expression of SYP protein and SYP m RNA in prefrontal lobe of D group was significantly lower than that in D group (P0.01), DE group was significantly higher than that in D group (P0.05). The expression of PSD-95 protein and its m RNA in prefrontal lobe of group D were significantly lower than those in group C (P0.01P 0.05). There was no significant difference in PSD-95 m RNA expression between DE group and D group, but the expression of PSD-95 protein in DE group was significantly higher than that in D group (P0.05). Conclusion: aerobic exercise can inhibit the expression of synaptophysin SYP,PSD-95 in the prefrontal lobe of rats during aging induced by D-gal to some extent, and reduce the degree of decrease in the expression of synaptophysin in the prefrontal lobe of rats. This may be one of the molecular mechanisms by which aerobic exercise delays the decline of brain aging learning and memory.
【作者单位】：西南民族大学体育学院;成都体育学院运动医学与健康学院;
【基金】：国家自然科学基金(31371202) 中央高校基本科研业务费专项资金(2017NZYQN22)
【分类号】：R87

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