耐力运动8周对大鼠骨骼肌收缩功能和线粒体生物合成的影响及机制

发布时间：2018-11-11 17:06

【摘要】：目的探讨8周耐力运动对不同类型骨骼肌收缩功能及线粒体能量代谢能力的影响及其机制。方法分离经8周平台跑步训练的♂SD大鼠的比目鱼肌和趾长伸肌,观测给予不同方式电刺激后两种类型骨骼肌收缩能力和抗疲劳能力的变化以及ATP含量和线粒体生物合成相关指标的改变。结果耐力运动8周可一定程度提高比目鱼肌和趾长伸肌在单次电刺激和强直电刺激下的收缩力,明显改善比目鱼肌的抗疲劳能力。ATP含量在两类肌肉中均明显升高,但只有比目鱼肌线粒体DNA、PGC-1α、NRF基因的转录及PGC-1α蛋白明显上调,并伴随p-AMPK/AMPK蛋白比值明显增加。结论耐力运动8周改善骨骼肌的收缩能力,但仅增加富含氧化型肌纤维的比目鱼肌的抗疲劳能力,这可能与耐力运动激活氧化型肌纤维的AMPK,上调PGC-1α转录和表达,增加线粒体生物合成有关。
[Abstract]:Objective to investigate the effects of 8 weeks endurance exercise on contractile function and mitochondrial energy metabolism of different skeletal muscles. Methods the soleus and extensor digitorum longus muscles were isolated from SD rats after 8 weeks of treadmill training. The changes of contractility and anti-fatigue ability of two types of skeletal muscle and the changes of ATP content and mitochondrial biosynthesis were observed after different modes of electrical stimulation. Results Endurance exercise for 8 weeks could improve the contractility of soleus muscle and extensor digitorum longus muscle under single electric stimulation and tetanus stimulation, and improve the anti-fatigue ability of soleus muscle obviously. The content of ATP was significantly increased in both groups of muscles. However, only mitochondrial DNA,PGC-1 伪, NRF gene transcription and PGC-1 伪 protein were up-regulated in soleus muscle, and the ratio of p-AMPK/AMPK protein was increased significantly. Conclusion Endurance exercise can improve the contractility of skeletal muscle for 8 weeks, but only increase the anti-fatigue ability of soleus muscle which is rich in oxidized muscle fiber, which may be related to the up-regulation of PGC-1 伪 transcription and expression by AMPK, activated by endurance exercise. Increased mitochondrial biosynthesis is involved.
【作者单位】：广州医科大学药学院广州蛇毒研究所;
【基金】：国家自然科学基金资助项目(No 81170778) 广东省科技计划(No 2013B21800098) 广州市科技计划(No2014J4100067)
【分类号】：R87

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