积极性恢复对兔骨腱结合部过度使用性损伤愈合的影响
发布时间：2018-11-16 20:11
【摘要】：过度使用性损伤在运动员中非常多见,运动员长期大强度训练,使骨腱结合部经常处于过度牵拉状态,导致损伤发生。而此类损伤的治疗较为困难,为运动员的训练比赛都带来了较大影响。很多运动员在损伤发生后,未能进行积极休息和治疗,继续带伤训练,反而运动成绩下降。因此,本研究旨在观察运动员在损伤发生后,应该选择休息还是继续训练,并如何进行训练,为对于发生此类损伤的运动员制定训练计划提供理论依据。 研究对象及方法：研究对象为28只成年雌性新西兰大白兔(18周龄,体重为2.6±0.2kg),随机筛选分为积极性恢复组(LL组,n=8),伤后休息组(LR组,n=8),损伤对照组(LC组,n=8)和空白对照组(CON, n=4)四个组别,CON组左右侧后肢均为对照,LL组、LR组和LC组左后肢进行循环载荷,前四周的电刺激强度均为兔后肢股四头肌最大收缩力的30%(即7.06±0.71N),每次2小时,每周3次；在此基础上,LR组休息4周,LL组继续以损伤阈下强度(最大强直收缩力的12%,即2.82±0.28N)训练4周。LR组、LL组和CON组在8周时取材,LC组在4周时取材,经过脱钙、脱水、石蜡包埋、切片、染色等处理,观察骨腱结合部组织学变化。 结果与分析：HE染色显示,LC组细胞计数显著高于CON组,LR组显著低于LC组,其余组间无差异：纤维软骨带厚度,LC组、LR组、LL组均显著高于CON组,LR组和LL组显著高于LC组,其余组间无差异；VEGF计数,LC组显著高于CON组,LR组、LL组显著低于LC组；其余组间无显著差异；bFGF计数,LC组、LR组、LL组均显著高于CON组,LL组显著高于LR组,其余组间无差异。LC组各项指标的变化,证实了4周造模的成功,LR组和LL组的变化说明休息或是损伤阈下强度训练都能够促进损伤的修复。而在bFGF计数中,LL组的变化提示在还没有恢复到损伤前,bFGF表达量仍显著增高,可能是损伤阈下强度的训练,继续刺激了肌腱组织的修复过程。 结论：通过4周的循环载荷损伤造模,进行4周的休息或4周的损伤阈下强度训练均可对骨腱结合部的损伤有明显的修复作用；但是4周的休息和阈下强度训练这两种方式对组织愈合的影响不同。
[Abstract]:Overuse injury is very common in athletes. The long term intensive training makes the bone-tendon junction often in an overstretched state, leading to injury. The treatment of this kind of injury is more difficult, which has a great impact on the training competition of athletes. After injury, many athletes failed to take active rest and treatment, and continued to train with injuries. Instead, their performance declined. Therefore, the purpose of this study is to observe whether athletes should choose to rest or continue to train after the injury, and how to train, so as to provide a theoretical basis for the athletes who suffer such injuries to make training plans. Methods: Twenty-eight adult female New Zealand white rabbits (18 weeks old, weight 2.6 卤0.2kg) were randomly divided into two groups: positive recovery group (LL group, nong8) and post-injury rest group (LR group, nong8 group). The left and right hind limbs in the CON group were all controlled, and the left hind limbs in the LL group, LR group and LC group were subjected to cyclic loading, respectively, in the four groups of injured control group (LC group, nong8 group) and blank control group (CON, nong4 group). The intensity of electrical stimulation in the first four weeks was 30% of the maximal contractility of the quadriceps femoris muscle of the hind limb (i.e. 7.06 卤0.71 N), 2 hours each time, 3 times a week. On this basis, the LR group rested for 4 weeks, the LL group continued to train for 4 weeks with the subthreshold intensity of injury (the maximum contractile force of rigidity, i.e. 2.82 卤0.28N). The LR group, LL group and CON group took the material at 8 weeks, and the LC group took the material at 4 weeks after decalcification. Dehydration, paraffin embedding, section and staining were used to observe the histological changes of bone tendon junction. Results and analysis: HE staining showed that the cell count of LC group was significantly higher than that of CON group, and that of LR group was significantly lower than that of LC group. There was no difference between other groups: the thickness of fibrochondrocytes, LC group, LR group and LL group were significantly higher than that of CON group. LR group and LL group were significantly higher than LC group, the other groups had no difference. VEGF counts in LC group were significantly higher than those in CON group, LR group and LL group were significantly lower than those in LC group, but there was no significant difference between other groups. BFGF count, LC group, LR group, LL group were significantly higher than CON group, LL group was significantly higher than LR group, and there was no difference between other groups. Changes in LR group and LL group showed that rest or subthreshold intensity training could promote the repair of injury. In the bFGF count, the changes in LL group indicated that the expression of bFGF was still significantly increased before the injury was recovered, which may be due to the training of subthreshold intensity of injury, which continued to stimulate the repair process of tendon tissue. Conclusion: after 4 weeks of cyclic load injury model, 4 weeks rest or 4 weeks of subthreshold strength training can significantly repair the injury of bone tendon junction. But four weeks of rest and subliminal intensity training had different effects on tissue healing.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R873
本文编号：2336525
[Abstract]:Overuse injury is very common in athletes. The long term intensive training makes the bone-tendon junction often in an overstretched state, leading to injury. The treatment of this kind of injury is more difficult, which has a great impact on the training competition of athletes. After injury, many athletes failed to take active rest and treatment, and continued to train with injuries. Instead, their performance declined. Therefore, the purpose of this study is to observe whether athletes should choose to rest or continue to train after the injury, and how to train, so as to provide a theoretical basis for the athletes who suffer such injuries to make training plans. Methods: Twenty-eight adult female New Zealand white rabbits (18 weeks old, weight 2.6 卤0.2kg) were randomly divided into two groups: positive recovery group (LL group, nong8) and post-injury rest group (LR group, nong8 group). The left and right hind limbs in the CON group were all controlled, and the left hind limbs in the LL group, LR group and LC group were subjected to cyclic loading, respectively, in the four groups of injured control group (LC group, nong8 group) and blank control group (CON, nong4 group). The intensity of electrical stimulation in the first four weeks was 30% of the maximal contractility of the quadriceps femoris muscle of the hind limb (i.e. 7.06 卤0.71 N), 2 hours each time, 3 times a week. On this basis, the LR group rested for 4 weeks, the LL group continued to train for 4 weeks with the subthreshold intensity of injury (the maximum contractile force of rigidity, i.e. 2.82 卤0.28N). The LR group, LL group and CON group took the material at 8 weeks, and the LC group took the material at 4 weeks after decalcification. Dehydration, paraffin embedding, section and staining were used to observe the histological changes of bone tendon junction. Results and analysis: HE staining showed that the cell count of LC group was significantly higher than that of CON group, and that of LR group was significantly lower than that of LC group. There was no difference between other groups: the thickness of fibrochondrocytes, LC group, LR group and LL group were significantly higher than that of CON group. LR group and LL group were significantly higher than LC group, the other groups had no difference. VEGF counts in LC group were significantly higher than those in CON group, LR group and LL group were significantly lower than those in LC group, but there was no significant difference between other groups. BFGF count, LC group, LR group, LL group were significantly higher than CON group, LL group was significantly higher than LR group, and there was no difference between other groups. Changes in LR group and LL group showed that rest or subthreshold intensity training could promote the repair of injury. In the bFGF count, the changes in LL group indicated that the expression of bFGF was still significantly increased before the injury was recovered, which may be due to the training of subthreshold intensity of injury, which continued to stimulate the repair process of tendon tissue. Conclusion: after 4 weeks of cyclic load injury model, 4 weeks rest or 4 weeks of subthreshold strength training can significantly repair the injury of bone tendon junction. But four weeks of rest and subliminal intensity training had different effects on tissue healing.
【学位授予单位】：北京体育大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R873
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