不同时长高强度间歇训练与中等强度持续运动对大鼠骨骼肌AMPK、PGC-1α表达量及最大摄氧量的影响

发布时间：2018-11-23 12:05

【摘要】：目的:探讨不同时长高强度间歇训练(high intensity interval training,HIIT)与中等强度持续运动对骨骼肌氧化能力相关因子AMPK和PGC-1α表达量及最大摄氧量(VO2max)的影响,为制定有效的运动健身负荷提供参考。方法:120只6周龄SPF级雄性Wistar大鼠按体重随机分为安静对照组、中等强度持续运动组与HIIT组三组,每组各40只。安静对照组大鼠不运动,中等强度运动组以60%~70%VO2max强度进行持续训练,HIIT组以50%VO2max、70%VO2max、90%VO2max的强度交替训练。跑台训练每周5天,每天50分钟。每周日上午8:00~9:00进行体重测量。每组大鼠分别在训练2、4、6、10周后取比目鱼肌,每组每次随机选取10只大鼠,取材前均进行VO2max测试。随后采用Western Blot检测比目鱼肌中AMPK、PGC-1α蛋白的表达情况。结果:(1)大鼠运动周数和运动方式分别对大鼠体重具有非常显著的影响且具有非常显著的交互作用(P0.01);(2)相同训练周期下,10周HIIT组大鼠骨骼肌PGC-1α含量显著高于安静对照组(P0.05),VO2max显著高于安静对照组和中等强度运动组;(3)相同训练方式下,10周HIIT组大鼠VO2max与0、2、4、6周时相比均有显著增高(P0.05),中等强度持续运动组训练至第4周时骨骼肌AMPK蛋白表达量显著高于第2周和第6周,同时显著高于2周安静组(基准值)(P0.05),HIIT组第2至第10周期间PGC-1α蛋白表达量均显著高于2周安静对照组(基准值)(P0.05);(4)10周内HIIT组PGC-1α蛋白表达量与最大摄氧量时序性变化趋势具有显著相关关系(P0.05)。结论:(1)10周HIIT可以有效提高大鼠骨骼肌AMPK和PGC-1α的蛋白表达量以及最大摄氧量;(2)10周HIIT相对于传统中等强度持续有氧运动对提高骨骼肌氧化能力和机体心肺耐力更为快速有效。
[Abstract]:Objective: to investigate the effects of (high intensity interval training,HIIT and moderate intensity continuous exercise on the expression of AMPK, PGC-1 伪 and maximal oxygen uptake (VO2max) in skeletal muscle. To provide a reference for the formulation of effective exercise load. Methods: 120 6-week-old SPF male Wistar rats were randomly divided into three groups according to their body weight: moderate intensity continuous exercise group and HIIT group with 40 rats in each group. The rats in the quiet control group did not exercise, the moderate exercise group received continuous training with 60%~70%VO2max intensity, and the HIIT group trained alternately with the intensity of 50VO2maxand 70VO2max90VO2max. Treadmill training 5 days a week, 50 minutes a day. Every Sunday morning at 8: 00 and 9: 00, the weight is measured. The soleus muscle was taken from each group after training for 10 weeks. 10 rats in each group were randomly selected for VO2max test. Western Blot was used to detect the expression of AMPK,PGC-1 伪 protein in soleus muscle. Results: (1) the number of weeks and the way of exercise had significant effects on the body weight of rats (P0.01). (2) under the same training period, the PGC-1 伪 content of skeletal muscle in HIIT group was significantly higher than that in quiet control group (P0.05), VO2max was significantly higher than that in quiet control group and moderate intensity exercise group. (3) under the same training mode, the VO2max of the HIIT group was significantly higher than that of the control group at the 6th week (P0.05), and the expression of AMPK protein in the skeletal muscle in the moderate intensity continuous exercise group was significantly higher than that in the 2nd and 6th week training at the 4th week. The expression of PGC-1 伪 protein in the), HIIT group was significantly higher than that in the quiet control group (P0.05) during the 2nd to 10th week (P0.05). (4) there was a significant correlation between the expression of PGC-1 伪 protein and the temporal trend of maximal oxygen uptake in HIIT group within 10 weeks (P0.05). Conclusion: (1) HIIT can effectively increase the expression of AMPK and PGC-1 伪 protein and maximal oxygen uptake in skeletal muscle of rats at 10 weeks. (2) HIIT at 10 weeks was more rapid and effective in improving skeletal muscle oxidation ability and cardiopulmonary endurance than traditional moderate intensity continuous aerobic exercise.
【作者单位】：北京体育大学研究生院;北京体育大学教学实验中心;集美大学体育学院;北京体育大学运动人体科学学院;
【基金】：中央高校基本科研业务费专项资金资助课题(2014YB017) 国家“十二五”科技支撑计划(2012BAK21B02) 国家自然科学青年基金(31401017)
【分类号】：R87

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