自体外周血富白细胞-血小板纤维蛋白对兔关节外骨道内移植肌腱腱骨早期愈合的影响

发布时间：2018-11-27 21:09

【摘要】：目的:探讨兔关节外骨道内使用自体外周血富白细胞-血小板纤维蛋白(L-PRF)对移植肌腱早期腱骨愈合的影响。方法:30只健康新西兰大白兔平均分为实验组和空白对照组。将兔的半腱肌腱取出制成游离肌腱,植入同侧关节外胫骨近端的横向骨道内制成腱骨愈合模型。实验组加入自体血来源的L-PRF,对照组不加入L-PRF。分别于术后4、8、12周三个时间点处死实验动物取材,切片染色,行腱骨界面的组织形态学观察、Buark评级,并在高倍镜下行成纤维细胞计数并分析。结果:各个时间点实验组成纤维细胞数目均多于对照组,差异均具有统计学意义(P0.05);在3个时间点中,与对照组相比,实验组腱骨连接更加紧密,腱骨间隙更小,胶原纤维排列更有序规则;标志腱骨早期愈合的Sharpey样纤维在4周时的实验组即可观察到,而对照组8周时才可观察到,并且随着时间延长,两组Sharpey样纤维均持续增多;两组标本的腱骨界面组织形态学Buark评级差异有统计学意义(P0.05)。结论:自体血来源的L-PRF可促进关节外骨道内移植半腱肌腱腱骨的早期愈合。
[Abstract]:Objective: to investigate the effect of autologous peripheral blood leukocyte-platelet fibrin (L-PRF) on tendon healing in the early stage of tendon graft. Methods: 30 healthy New Zealand rabbits were divided into experimental group and blank control group. The rabbit semitendinosus tendon was removed to make the free tendon and implanted into the transverse bone canal of the proximal tibia outside the ipsilateral joint to establish a tendon healing model. L-PRF from autologous blood was added in the experimental group, but not in the control group. The experimental animals were killed at 4 weeks and 8 weeks after operation at three time points. The specimens were stained with sections, the morphology of the interface of tendon and bone was observed, the Buark rating was evaluated, and the fibroblasts were counted and analyzed under high power microscope. Results: the number of fibroblasts in the experimental group was more than that in the control group at each time point, and the difference was statistically significant (P0.05). At three time points, compared with the control group, the tendon bone of the experimental group was more compact, the space of the tendon bone was smaller, and the collagenous fiber arrangement was more orderly than that of the control group. The Sharpey like fibers which marked the early healing of tendon bone could be observed in the experimental group at 4 weeks, but in the control group at 8 weeks, and the Sharpey like fibers in both groups continued to increase with the prolongation of time. There was significant difference in Buark rating of tendon and bone interface between the two groups (P0.05). Conclusion: L-PRF derived from autologous blood can promote the early healing of semitendinosus tendon bone graft.
【作者单位】：安徽医科大学第一附属医院运动创伤与关节镜科;
【基金】：安徽省自然科学基金(编号:1208085MH157)
【分类号】：R87

【相似文献】